FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001132210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324
Sequences flagged as poor quality0
Sequence length208
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCGCCTGCACTAAGAGAAACGCAACGAACAACTTTGTCAATGCATTGC5216.049382716049383No Hit
CGGGTCACCTTCTGAATTTTTACCTTCATAAACAGCAAGGACTGCGCTCT4915.123456790123457No Hit
CGATGTATCCAAGTCTGACGGCCCCAGAAACGGGTGTGCAGGGCGCCCAT309.25925925925926No Hit
GCCCTGTAATCCCAGCTCCCCGGGAGGCTGAGGTCGGCGGATCGTGGGTG268.024691358024691No Hit
GCCCTGTAATCCCAGCTACTTGGGAGGCTGAGGTGGGAGAATTGCTTGGT30.9259259259259258No Hit
GCCCTGTAATCCCAGCTACTCGGGAAGCTGAGGTGGAACAATCACTTGAA20.6172839506172839No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGCCCGAGGGT20.6172839506172839No Hit
GCCCTGTAATCCCAGCACTTTGGCGGGCTGAAGTGGGAAGATTGCTTGAG20.6172839506172839No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGCCCAAGTAC10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGACGGA10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGAGTAGTGCCCGAGGGG10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCACTCCT10.30864197530864196No Hit
ATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTACGGAAAACAT10.30864197530864196No Hit
GGTTTTTGAGGAATTGGATTTCGAGTTTGTTCGTAAGGTGAGATGTTTCG10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTCTAATCGGA10.30864197530864196No Hit
AGCTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGAGATGCAA10.30864197530864196No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAATCTTTAACAAGAACGAAAAA10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCAGGGGTA10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGCCGTCTCTGGCACA10.30864197530864196No Hit
CCAGCATCACCCATGCCTACAGTATTGTTATCGGTAGCAAGCACATCACC10.30864197530864196No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTCGTAGTGCTCCAGGGC10.30864197530864196No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGAAGTGATAATTTGACACACTGCT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGGCAGCGGCC10.30864197530864196No Hit
AGGGTTCCCTGGCCCCAGTAGTCCCGCCCGGAGTTACGTGTGGGTAGCAG10.30864197530864196No Hit
CAAACAGGCAAAAAATTTAGGGTCGGCATCAAAAGCAATATCAGGACCAA10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAATGCCCCACTGG10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACTCGAGTGG10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGCGCAAGGGT10.30864197530864196No Hit
CGGGTCACCTTCTGACTTTTTACCTTCATAAACAGCAAGGACTGCGCTCT10.30864197530864196No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTAGCTTAAGTGCCCGATGGC10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTATCCTCTGGGCCCGAGGTA10.30864197530864196No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATCGGACCCGAAGATCCG10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGAAGGGTTGAGCTATTGA10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGAGCCCGAGGGT10.30864197530864196No Hit
CGGTATCCAAGTCTGACGGCCCCAGAAACGGGTGTGCAGGGCGCCCATTG10.30864197530864196No Hit
GCCCTGTAATCCCAGCAATTCTGTTACCAGTGGAAGGTGCCCAGATTCTT10.30864197530864196No Hit
GCCCTGGAATCCCAGCTCCCCGGGAGGCTGAGGTCGGCGGATCGTGGGTG10.30864197530864196No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTCGTAGTAGTAGTGCCCAACAGA10.30864197530864196No Hit
AGCGTATCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTCTACT10.30864197530864196No Hit
GCCCTGTAATCCCAGCTTCTCGGGAGGCTGAGGCAGGAGAATCGCTTGAA10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGAGCAAAAGGT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAACGGAGGTG10.30864197530864196No Hit
CGGGTCACCTTCTGAATTTTTACCTTCATAAACAGCAATGACTGCGCTCT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACAGTAGTAGTAGTGCCCGTTAGT10.30864197530864196No Hit
CAGAGGCGCTGTGCCTGCATATCATCCACCACCACCCTAGCCCAGCCTTC10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTACCAGTA10.30864197530864196No Hit
CGATGTATCCAAGTCTGACGGCCCCAGGAACGGGTGTGCAGGGCGCCCAT10.30864197530864196No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAAGCAGGAGAAACAAGTTAGCGCTCA10.30864197530864196No Hit
GTGGTCCCCTGGCCCCAGACGTCCATAGCGTAGTAGTAGTACCCGCCCGT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACTAATATGG10.30864197530864196No Hit
GCTAAATACGTTAACAAAAAGTCAGATATGGACCTTGCTGCTAAAGGTCT10.30864197530864196No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAATTCCCACTCACAGGTACAAATATT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGCCCGTAGGG10.30864197530864196No Hit
GCCCTGTAATCCCAGCTACTCGGGAGGCTGAGACAGGAGAATCGCTTTAA10.30864197530864196No Hit
GCCCTGTAATCCCAGCTACTTGGGAGGCTAAGGCAGGAGAATCCTTGAAC10.30864197530864196No Hit
TCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCGGCTTAA10.30864197530864196No Hit
CGATGCATCCAAGTCTGACGGCCCCAGAAACGGGTGTGCAGGGCGCCCAT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCTTTTGTG10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACTGCCCGCCCGT10.30864197530864196No Hit
CGATGTAGCCAAGTCTGACGGCCCCAGAAACGGGTGTGCAGGGCGCCCAT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATGCCGTAGTAGTAGTGCCCGAGGGT10.30864197530864196No Hit
TTTACTTTTTATGTCCCTCATCGTCAAGTTTAGGGGGAACAGTGGAGTAA10.30864197530864196No Hit
GTGGATTACTATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAA10.30864197530864196No Hit
ACGCTACCTGTAGGAAGTGTCCGCATAAAGTGCACCGCATGGAAATGAAG10.30864197530864196No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTGGTAATAGTAAGGCCCTACGCG10.30864197530864196No Hit
AACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTACGGAAAA10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGCCGGAGGGG10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCTTGTGT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCTTTGCCA10.30864197530864196No Hit
GTGGTCCCTTGGCCCAAGACGTCCATACCGTAGTAGTAGTGCCCGAGGTA10.30864197530864196No Hit
TGTTTCAAGTATCGGCAACAGCTTTATCAATACGATGAAAAATAGCAATA10.30864197530864196No Hit
GAAGGTACGTTGCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGA10.30864197530864196No Hit
GGCTCTAATGTTCCTAACCCTGATGAGGCCGTCCCTAGTTTTGTTTCTGG10.30864197530864196No Hit
AGCAAGGTCCATATCTGACTTTTTGTTAACGCATTTAGCCACATAGAAAC10.30864197530864196No Hit
CATGACAAGTAAAGGACGGTTGTCAGCGCCATAAGAGGTTTTACCTCCAA10.30864197530864196No Hit
CGATGTATCCAAGTCTGGCTGCAGGGACCCAGGCCCAGGGGCAGTAACAC10.30864197530864196No Hit
ACGACCACAATTAGGGTCAACGCTACCTGTAGGAAGTGTCCGCATAAAGT10.30864197530864196No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGGAGCCCACCATATGGAAGTC10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGCCCGTTGGT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGCTAGAGGGC10.30864197530864196No Hit
CAAGTTACTGAACAATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAA10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGCCCGTAGGT10.30864197530864196No Hit
ATTGTCCCTTGGCCCCAGATATCAATATAGTAGCAACAGAACTCCCTATC10.30864197530864196No Hit
AATAACACTATAGACCACCGCCCCGAAGGGGACGAAAAATGGTTTTTAGA10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTCGGGCCCGATGGT10.30864197530864196No Hit
GCCCTGTAATCCCAGCGCTTTGGGAGGCTGAGACAGGCGGATCACGAGGT10.30864197530864196No Hit
TTTGTCCCTTTTCCCCATACTTCCATACCTTATTCTTCTTCCCCCTTCTT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGCCCGCGTCT10.30864197530864196No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGGGCCCGAGGGT10.30864197530864196No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTATTGCCCCATGAA10.30864197530864196No Hit
CGGCGTTATAACCTCACACTCAATCTTTTATCACGAAGTCATGATTGAAT10.30864197530864196No Hit
CGATGTATCCAAGTCTGAAGGAGAAGTGATGGAAGAGCGGTTCAGCAGGA10.30864197530864196Illumina Paired End PCR Primer 2 (95% over 21bp)
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAAAGACGATACGATATAAC10.30864197530864196No Hit
GATAATCCCAATGCTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGT10.30864197530864196No Hit
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCATAGTAGTAGTGCCCGAGGCA10.30864197530864196No Hit
TGTCGCCTGCACCAAGAGAAACGCAACGAACAACTTTGTCAATGCATTGC10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGCCCAAGAGT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGAGTAGTGCTT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATATCGTAGTAGTAGTGCCCGAGGGT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCCATACG10.30864197530864196No Hit
AGGGTTCCCTGGCCCCAGTAGCACCAACTGCTACTATACTAGATCTTTCG10.30864197530864196No Hit
GTTAAAATTTTGGGTTATTTTTTGAATTTTTATTTAGATCGGAAGAGCGG10.30864197530864196No Hit
AAGACTCAAAGCGAACCAAACAGGCAAAAAATTTAGGGTCGGCATCAAAA10.30864197530864196No Hit
GCCCTGTAATCCCAGCTACTTGGGAGGCTGAGACAGGAGAATCACTTGAA10.30864197530864196No Hit
GCCCTGTAATCCCAGCTCCAGTCAGGGCAGAGGGCAGGGTCAGGGGCACG10.30864197530864196No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATAAATTACCACTCTATCAAAAAGAA10.30864197530864196No Hit
TTTGTATGGCAACTTGCCGCCGCGTGACATTTCTATGAAGGATGTTTTCC10.30864197530864196No Hit
GCAGTCGGGAGGGTAGTCGGAACCGAAGAAGACTCAAAGCGAACCAAACA10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGCCCGATGAC10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCCGCGGGT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGCCCGCGCGT10.30864197530864196No Hit
ATGAAGGATGGTGTTAATGCCACTCCTCTCCCGACTGTTAACACTACTGG10.30864197530864196No Hit
CGGGTCACCTTCTGAATTTTTACATTCATAAACAGCAAGGACTGCGCTCT10.30864197530864196No Hit
GCCCTGTAATCCCAGCTATTCAGGAGGCTGAGGCAGGAGGATCGCTTGAG10.30864197530864196No Hit
TGATGTATCCAAGTCTGACGGCCCCAGACACGGGTGTGCAGGGCGCCCAT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGCTCGAGGGT10.30864197530864196No Hit
AGGGTTCCCTGGCCCCAGGAGTCAAAACCCTTCCCAGAGCTCTTTCCTCG10.30864197530864196No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGGGGATAGCACCTTGAACTAT10.30864197530864196No Hit
CGCTTTCCTGCTCCTGTTGAGTTTATTGATGCCGTCATTGCTTATTATGT10.30864197530864196No Hit
GCCCTGTAATCCCAGCTACTAGGGAGGCTGAGGCAGGAGAATCGTTTGAG10.30864197530864196No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTACTTGGACCTTACTAC10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGCCCTAGGGC10.30864197530864196No Hit
ACGAAGGCGCATAACGATACCACTGACCCTCAGCAATCTTAAACTTCTTA10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGACCCGAGGGT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACAGTAGTAGTAGTGCCCGAGGGT10.30864197530864196No Hit
TGTCGCCTGACTAAGAGAAACGCAACGAACAACTTTGTCAATGCATTGCA10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTTCTCCC10.30864197530864196No Hit
GTGATCCCTTGGCCCCAGACGTCCATACCGTAATAATTGCCCCGCCTACC10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCAGAGGGA10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTACTAGTACCAGTCAAGGTA10.30864197530864196No Hit
CGGGTCACCTTCTGAATTTTTACCTTCATAAACAGCAAGGAATGCGCTCT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTCGTGCCCAACCAT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATCGTAGATA10.30864197530864196No Hit
ATGCGGCATACGCTCGGCGCCAGTTTGAATATTAGACATAATTTATCCTC10.30864197530864196No Hit
GCAACGTACCTTCAAGAAGTCCTTTACCAGCTTTAGCCATAGCACCAGAA10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCGTGTGC10.30864197530864196No Hit
CGGTGCACTTTATGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTT10.30864197530864196No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGGAGATAGCAGCTGGTACTAC10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAGGCGGAGA10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGGCCGTGTTT10.30864197530864196No Hit
GCTAAAGGTTAGCCGCTTAAAGCTACCAGTTATATGGCTGTTGGTTTCTA10.30864197530864196No Hit
GCATACGCTCGGCGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTA10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTGAAAGATTGGCCAGCTGGT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCAAAGGCA10.30864197530864196No Hit
TATCACGAAAATAGTCACGCAAAGCATTGGGATTATCATAAAACGCCTCT10.30864197530864196No Hit
GCTTCTGCCTTTTTGGATTTACCCGTAGATTATTTCTATTTTCTTACGAG10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTATCAGTGCCTAAGGGT10.30864197530864196No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGCCGTAGTAGTAGTTCCCGTTATA10.30864197530864196No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTACA10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGAAGGGACCCATGGCTAA10.30864197530864196No Hit
GGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCTACCAG10.30864197530864196No Hit
AGGGTTCCCTGGCCCCAGTAGTCCAGGGGGGCTACCACCACTACTCGTGG10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCCGAACTCCCCGAACCATAGTA10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTCGTGCCCTAGGTT10.30864197530864196No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTGCCCGCGGGT10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGCCGTGGGATAACCA10.30864197530864196No Hit
CCAGCGTACCATAAACGCAAGCCTCACCGCAGCGACGAGCACGAGAGCGG10.30864197530864196No Hit
GCCCTGTAATCCCAGCTACTGGGAGGCTGAGGCAGGAGAATCGCTTGAAC10.30864197530864196No Hit
ATGGTCCCTTGGCCCCAGACGTCCATACCGCAGTAGTAGGGCCCGGTGGA10.30864197530864196No Hit
GCCCTGTAATCCCAGCTACTCCAGAGGCTGAGGCAGGAAAATCACTTGAA10.30864197530864196No Hit
GCCCTGTAATCCCAACTACTTGGGACGCTGAGGCAGGAGAATTGCTTGAG10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTCGTGGTAGTGCCCGAGAGG10.30864197530864196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGCCCGAGGGC10.30864197530864196No Hit
GTGGCAAGAAAAGCGGCATGGTCAATATAACCAGTAGTGTTAACAGTCGG10.30864197530864196No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGCCGTAGTAGTAGTACCTAGGGAT10.30864197530864196No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)