FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001132212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126
Sequences flagged as poor quality0
Sequence length208
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTTTGAGGAATTGGATTTTGAGTTTGTTTGTAAGGTGAGATGTTTTG107.936507936507936No Hit
TTTTTATTTTTATAAATAGTAAGGATTGTGTTTTTTTGTATGGTGTTTTG64.761904761904762No Hit
GTTTGTAATTTTAGTTTTTTGGGAGGTTGAGGTTGGTGGATTGTGGGTGT64.761904761904762No Hit
GGTTTTTGAGGAATTGGATTTCGAGTTTGTTCGTAAGGCGAGACGTTTCG43.1746031746031744No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTATTCATTTTTCGGCGGGACCAG21.5873015873015872No Hit
ATTGTAGAAAGAGTTTGGGAATAGTTAAGGGATAAAAGTTGTGATGATGG21.5873015873015872No Hit
GTTAAAATTTTGGGTTAAGGAGATCGACGCGTTGTCGTTTGTATTAAGAG21.5873015873015872No Hit
GTTTGTAATTTTAGTTTTTTGGGAGGTTGAGGTAAGAGAATCGTTTGAAT10.7936507936507936No Hit
ATTGTAGAAAGAGTTTGGGAGATAGAGTGAGATTGTTAAAAAAAAAAAAA10.7936507936507936No Hit
AGGATGCAATGGACCCAGAAGAAGAACTATACCAGCGTAAGCAATAGCAC10.7936507936507936No Hit
CTGGGTTATGAGGTGACGCCGTTCAAATAGATATTGAAGCAGAACGTAAA10.7936507936507936No Hit
GTTTGTAATTTTAGTTTTTTGGGAGGTTGAGGTTGGTGGATTGTAGGTGT10.7936507936507936No Hit
AAAATAATCAGCGTGACATTGAGAAGGGTAATAAGAACGAACTATAAAAA10.7936507936507936No Hit
GTTAAAATTTTGGGTTAAGGAGATGTAGATATGGTGTTAGTGATTGAAGG10.7936507936507936No Hit
GAGTGGTCGTCAGATTGCGATAAACGGTCACATTAAATTTAACCTGACTA10.7936507936507936No Hit
AATGCTTGGGAGCGTGCTGGTGCTGATGCGTCCTCTGCTGGTATGGTTGA10.7936507936507936No Hit
ATTGTAGAAAGAGTTTATGGGGAAAAGTTGTTAGAGTAATGAATGTTTTA10.7936507936507936No Hit
GTTTGTAATTTTAGTTTTTTGGGAGGTTGAGGATGGTGGTTTGTGGGAGT10.7936507936507936No Hit
AGGGTGCCACGGCCCCAGAGATCGAAGTACCAACCAATAACCGACCCAGG10.7936507936507936No Hit
TAAACATCATAGGCAGTCGGGAGGGTAGTCGGAACCGAAGAAGACTCAAA10.7936507936507936No Hit
CAAGCTATTTAACTGGCGGCGATTGCGTACCCGACGACCAAAATTAGGGT10.7936507936507936No Hit
GAGAGAGAGAGAGGAAAAAAAGAAGAAGCGGAAAAAAAAAGAAGAAAAGA10.7936507936507936No Hit
ATTGTAGAAAGAGTTTGGGATTGGATAAATGTTAGTTTTTTGGTTATTTT10.7936507936507936No Hit
TGTAGGCATGGGTGATGATGGTATTAAATCTGCCATTCAAGGGTCTAATG10.7936507936507936No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTACCACCGGAT10.7936507936507936No Hit
AAGGCTGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTACTGAGAAGTT10.7936507936507936No Hit
GTTAAAATTTTGGGTTAAGGAGATTGACGCGTTGTCGTTTGTGTTAAGAG10.7936507936507936No Hit
GTTAAAATTTTGGGTTAAGGAGATTGACGCGTTGTCGTTTGTATTAAGAG10.7936507936507936No Hit
TTCGGTTCCGACTACCCTCCCGACTGCCTATGATGTTTATCCTTTGGATG10.7936507936507936No Hit
GTTAAAATTTTGGGTTAAGGAGATCGACGCGTTGTTGTTTGTATTAAGAG10.7936507936507936No Hit
GGTTTTTGAGGAATTGGATTTTGAGTTTGTTTGTAAGGTGAGACGTTTTG10.7936507936507936No Hit
AGGGTTCCCTGGCCCCAGGTGTTCCAGCTGGTACTACTACGATCTCTCGC10.7936507936507936No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGTCTTCGACTCCCCGAA10.7936507936507936No Hit
GAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTACG10.7936507936507936No Hit
GCAAGCTGGCTGCTGAACGCCCTCTTAAGGATATTCGTGATGAGTATAAT10.7936507936507936No Hit
CCCTAGTTTTGTTTCTGGTGCTATGGCTAAAGCTGGTAAAGGACTTCTTG10.7936507936507936No Hit
GTTTGTAATTTTAGTTTTTTGGGAGGTTGAGGTAGGTGGATTATAAGGTT10.7936507936507936No Hit
GGTTTTTGAGGAATTGTATTTTGAGTTTGTTTGTAAGGTGAGATGTTTTG10.7936507936507936No Hit
GAAGATGATTTCGATTTTCTGACGAGTAACAAAGTTTGGATTGCTACTGA10.7936507936507936No Hit
AGGGTTGGGAGGGCGCAGGAGAAGGAACAGCAACTACTGGCACAGTTGAG10.7936507936507936No Hit
AGGGTTCCCTGGCCCCAGTAGTCAACGCAATGCCGCCCAATAACCAGTCA10.7936507936507936No Hit
ATTGTAGAAAGAGTTTGGAAGAAGTAATCGTAGATGTGGTGGAAATAGCG10.7936507936507936No Hit
AGAAACAACCTGATTAGCGGCGTTGACAGATGTATCCATCTGAATGCAAT10.7936507936507936No Hit
GCGGTTCCTGAATGAATGGGAAGCCTTCAAGAAGGTGATAAGCAGGAGAA10.7936507936507936No Hit
GTTTGTAATTTTAGTTTTTCGGGAGGTTGAGGTTGGCGGATCGTGGGTGT10.7936507936507936No Hit
GACGAATCACCAGAACGGAAAACATCCTTCATAGAAATTTCACGCGGCGG10.7936507936507936No Hit
GGTTTTTGAGGAATTGGATTTATATAGTATGTATTGTTTTGTGTTTGTAT10.7936507936507936No Hit
GTTTGTAATTTTAGTTTTTTCCTTTCCAGGTATTAATATGGTATTTTAGA10.7936507936507936No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTCCCTCTTGGGAGCCTC10.7936507936507936No Hit
ATTGTAGAAAGAGTTTGGGAGAGTTGTAATGTAGGTGTAGTTTTTGTTAG10.7936507936507936No Hit
AATTTTTGACGCACGTTTTATTATGTGTAAGTAAGAACGTTAGTGTTTAT10.7936507936507936No Hit
ATTGTAGAAAGAGTTTGGGAGATTATTAGGATTTTTTTTTTAAGATGGTT10.7936507936507936No Hit
TGGGGAAAGGGAAAAAAGGAGAGGGAGAAGGAAGCGGAAGGGCGAAGGGG10.7936507936507936No Hit
CTAGGACGTTGACGGAAGAGACCAAAAGTCGATAGGCAAGGTAGCTGGTA10.7936507936507936No Hit
ACCATACGACCAATATCACGAAAATAGTAAAGAAAAGCATTGGGATTATA10.7936507936507936No Hit
ATTGTAGAAAGAGTTTGGGAGATAGAGTAAGATTGTTTTAAATAAAGTAA10.7936507936507936No Hit
CGGGTCACCTTCTGAATTTTACACTCTTTCCCTACACGACGCTCTTACAT10.7936507936507936Illumina Single End PCR Primer 1 (96% over 29bp)
ATTGTAGAAAGAGTTTGGGAGATTTTTTTTTATTTTTTATAGTTGTATTT10.7936507936507936No Hit
GTTTGTAATTTTAGTTTTTCGGGAGGTTGAGGTCGGCGGATCGTGGGTGT10.7936507936507936No Hit
GCAAGTTGCCATACAAAACAGGGTCGCCAGCAATATCGGTATAAGTCAAA10.7936507936507936No Hit
GTTTGTAATTTTAGTTTTTTGGGAGGTTTAGGTAGGTAGATTATGAGGTT10.7936507936507936No Hit
GCTTGTAATTTTAGTTTTTTGGGAGGTTGAGGTTGGTGGATTGTGGGTGT10.7936507936507936No Hit
ATTGTAGAAAGAGTTTGGGAGATTGAGGTTGGAGGATTGTTTGAGTTTAG10.7936507936507936No Hit
ATTGTAGAAAGAGTTTGGGAGATTGAAGTTGTAGTGAGTTATGATTATAT10.7936507936507936No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTACTGCACCGTACCCCGAAC10.7936507936507936No Hit
TGGGTGCATGAGTATGGTACAGCTAATGGCCGTCTTCATTTCAATGAGGT10.7936507936507936No Hit
AAGCAGCCTTATGGCCGTCAACATACATATCACCATTATCGAACTCAACG10.7936507936507936No Hit
AAAGTGCACCGCATGGAAATGAAGATGGTAATTAGATGTAACATAATCAG10.7936507936507936No Hit
CCATCATGGCGACCATCCAAAGGATAAACATCATAGGCAGTCGGGAGGGT10.7936507936507936No Hit
GTTTGTAATTTTAGTTTTTTGGGAGGTTGAGGAGGAGGGTGGTTTGAGTT10.7936507936507936No Hit
TTATCGCTTCCATGACGCAGAAGTTAACACTTTCGGATATTTCTGATGAG10.7936507936507936No Hit
GGCTTTTTGACCGCCTCGAAACAATTTAGATATGGTGCCATCAGAAAGAG10.7936507936507936No Hit
GTTTGTAATTTTAGTTTTTTGGGAGATTGAGGTGGGTAGATTATTTGAGG10.7936507936507936No Hit
ATTGTAGAAAGAGTTTGAGTGTATTTTTTGAGAATTTAGGATTTAATATT10.7936507936507936No Hit
ATTGTAGAAAGAGTTTGGGAGAGTTTTTTTAATAGAATATTATAGGTTGG10.7936507936507936No Hit
ATGTAGAAAGAGTTTGGGAGGTGGAGGTTTTACGATTATATTATTGTATT10.7936507936507936No Hit
GTTTGTAATTTTAGTTTTTTAGAAGGTAGGGACGATTAAGGGTGTATAAA10.7936507936507936No Hit
ATTGTAGAAAGAGTTTGGGAGGTTAAGGTTGTAGTGAGATTTGATTGAGT10.7936507936507936No Hit
GGTTTTTGAGGAATTGGATTTTGAGTTTGTTTGTAGGGTGAGATGTTTTG10.7936507936507936No Hit
ATTGTAGAAAGAGTTTGGGGGATAAGGTTTGTTTGGTTGTTTTTTGTTTA10.7936507936507936No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTCGTAGTGGGAATTACCCCC10.7936507936507936No Hit
GGTTTTTGAGGAATTGGATTTTGAGTTTGTTTGTAAGGTGAGGTGTTTTG10.7936507936507936No Hit
TCTTGATAAAGCAGGAATTACTACTGCTTGTTTACGAATTAAATCGAAGT10.7936507936507936No Hit
ATTGTAGAAAGAGTTTGGGAGATTGAGGGGTGTAGAGTGGGGTTGGATTA10.7936507936507936No Hit
TTAAGCGGCTCACCTTTAGCATCAACAGGCCACAACCAACCAGAACGTGA10.7936507936507936No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTCCCGGCATACACCACCAGT10.7936507936507936No Hit
GTTTGTAATTTTGGTTTGGTGTAGTGGTGAGTGTTTGTAGTTTTAGTTAT10.7936507936507936No Hit
ATTGTAGAAAGAGTTTGGGAGATTGAGGGGCGTAGAGTGGGGTTGGATTA10.7936507936507936No Hit
TGGGTTCCCTGGCCCCAGGGGTCGAACCAGGAGATAGCAGCTGGTACTAC10.7936507936507936No Hit
GAAAAAAATGTGACTGATATCTAAACAAGTACTTGACGAACGTGCCAAGT10.7936507936507936No Hit
ATTGTAGAAAGAGTTTGGGAGATAGAGTGAGATTTTGTTTTAATAAATAA10.7936507936507936No Hit
GTTTGTAATTTTAGTTTTTTAGGAGGTTGAGGTAGGAGGATCGTTTGAGT10.7936507936507936No Hit
GTTAAAATTTTGGGTTAAGGAGATTGACGTGTTGTTGTTTGTATTAAGAG10.7936507936507936No Hit
GTTTGTAATTTTAGTTTTTTGAGATGTTGAGGCGGGAGGTGGAGTTTTTA10.7936507936507936No Hit
ATTGTAGAAAGAGTTTGGGAGAAGATGGTTTTTAGAAGATCGGAAGAGCG10.7936507936507936No Hit
ATTGTAGAAAGAGTTTGGGAAGGATTTTTTTTAAGATGGTTTTTAGAAGA10.7936507936507936No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGAGTCTC10.7936507936507936No Hit
GTTTCCTGCGCGTACACGCAAGGTAAACGCGAACAATTCAGCGGCTTTAA10.7936507936507936No Hit
CTTCTCAGTAACAGATACAAACTCATCACGAACGTCAGAAGCAGCCTTAT10.7936507936507936No Hit
ATTGTAGAAAGAGTTTGGGATTGGCATGTAGTGGGTATTGGTAGTAGGTA10.7936507936507936No Hit
TTTTTATTTTTATAAATAGTAAGGATTGCGTTTTTTTGTATGGTGTTTTG10.7936507936507936No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)