FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001132214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11985
Sequences flagged as poor quality0
Sequence length208
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGGGATAGTGGAG1721.4351272423863162No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTAGCAGCTGGTACTACTACA1100.917813934084272No Hit
GTTCAGCAGGAATGCCGAGACCGATCTCTGTCTATCATCTCGTATGCCGT620.5173133083020443Illumina Paired End PCR Primer 2 (96% over 28bp)
TCCCCAGTCAGGGTGAGTAGTGGTGGGGAGCCGGGCAGGGGCCCAGCCCT520.4338756779307468No Hit
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC450.37546933667083854No Hit
ACGAAGGCGGCAAAGGCGACGGAATGGAGGAGGTGCCTCACGGTAAGCTC400.3337505214851898No Hit
ACAGGTGGAGAACACTGACAGCATCCTTTTTTCACAGGCGACACACACCA360.3003754693366708No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA230.19190654985398414No Hit
CACACCAAGCTCAGAGAGGGCAGGGGTGGGAAGGAGACATCAGATGTGGG150.1251564455569462No Hit
CGGCAGCAGCACATGGCACCCGCGCAGAGGCAGTGCAGGCAGCCCGGGCC150.1251564455569462No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTATTCAGCCACGGTGAGTCAGCC140.11681268251981644No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATTC120.10012515644555695No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)