FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001132216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16183
Sequences flagged as poor quality0
Sequence length208
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC780.48198727059259716No Hit
TCCCCAGTCAGGGTGAGTAGTGGTGGGGAGCCGGGCAGGGGCCCAGCCCT690.42637335475498983No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA440.27189025520608046No Hit
ACGAAGGCGGCAAAGGCGACGGAATGGAGGAGGTGCCTCACGGTAAGCTC370.22863498733238585No Hit
GTTTGTAATTTTAGTTTTTTGGGAGGTTGAGGTTGGTGGATTGTGGGTGT280.17302107149477847No Hit
CACACCAAGCTCAGAGAGGGCAGGGGTGGGAAGGAGACATCAGATGTGGG260.1606624235308657No Hit
GTTCAGCAGGAATGCCGAGACCGATCTCTTACTCGCATCTCGTATGCCGT260.1606624235308657Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGCAGCAGCACATGGCACCCGCGCAGAGGCAGTGCAGGCAGCCCGGGCC250.15448309954890935No Hit
ACAGGTGGAGAACACTGACAGCATCCTTTTTTCACAGGCGACACACACCA220.13594512760304023No Hit
ATGCAGACACCACACGCTCTCCTGCAGTAACTCCTTTCATCTTCACGGTG200.12358647963912747No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATGGGG200.12358647963912747No Hit
GGTTTTTGAGGAATTGGATTTTGAGTTTGTTTGTAAGGTGAGATGTTTTG180.11122783167521473No Hit
GACGTGCTGTAGACGCGCAGGCCGCTCATGCTGGGGGTAGACAGACGGGC180.11122783167521473No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTATTCAGCCACGGTGAGTCAGCC180.11122783167521473No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)