FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001132217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218433
Sequences flagged as poor quality0
Sequence length208
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGGGATAGTGGAG5100.2334812047630166No Hit
TTCCCTGGGGGCTGACGGATCCAGTTCAGCAGGAATGCCGAGACCGATCT3030.13871530400626278Illumina Paired End PCR Primer 2 (96% over 29bp)
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC2580.11811402123305544No Hit
TTCCCTGGGGGCTGGCGGATCCAGTTCAGCAGGAATGCCGAGACCGATCT2230.10209080129833861Illumina Paired End PCR Primer 2 (100% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)