FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416646
Sequences flagged as poor quality0
Sequence length208
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTACA21001350.40562011875789No Hit
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC123122.9550265693178384No Hit
TCCCCAGTCAGGGTGAGTAGTGGTGGGGAGCCGGGCAGGGGCCCAGCCCT46521.1165353801548557No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTACC44421.0661328801908576No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA40120.9629277612169563No Hit
CACACCAAGCTCAGAGAGGGCAGGGGTGGGAAGGAGACATCAGATGTGGG33520.8045199041872477No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGCACTACTACA32460.7790786423006582No Hit
CCATCATAGCCCTGCTGACCCTGTGACCTTGTGGCAGCCCCTCTGGCCCC24380.5851490233915602No Hit
TGGGGACAGGGACAGGTGGTCCTGGGATCAGGCTTCAGCTGCACGTCCTG18800.45122238063007925No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGAACTACTACA18050.43322148778579417No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTCCA15820.3796988330621199No Hit
ATGCAGACACCACACGCTCTCCTGCAGTAACTCCTTTCATCTTCACGGTG12390.2973747497875895No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTCCTACA11990.28777427360397073No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGGACTACTACA10810.25945286886229557No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTACT10370.248892345060315No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTATA9790.23497165459406785No Hit
TCTGTGGCTGCTGCACGGCACAGCCGAGGAGAGCTGGGTCCAGGCCTGGC8990.21577070222683045No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCCTGATGGTACTACTACA8300.19920988081008817No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTAAA7980.19152949986319323No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTATTACA7670.1840891308208887No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGCTGGTACTACTACA7540.18096897606121265No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCACATGATGGTACTACTACA7090.1701684403546416No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTAATACA7090.1701684403546416No Hit
ATTTTTAGCCCTTGGCTAGGTGTGGGGCACATAGAAGGAGCCCCATCATG6600.15840785702970867No Hit
CAGGAAGGGGCTCTGGTGCCGGGCTGAGCGGGGGAAGCAGGGGTAGCGGA6580.15792783322052772No Hit
CTGTGGGCTAAGGTCTCCCTTTGGCCAGGCAGGGGTGCTGGAAGGCGGTC6470.1552877022700326No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTCCTACTACA6460.15504769036544214No Hit
TTGTGGCAGCCCCTCTGGCCCCTTGGCCCCGTCCTGTTACCTCTCCTCGT5700.1368067856165666No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGCACTACTACC5410.129846440383443No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGTTACTACTACA5320.1276863332421288No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACCACTACA4930.11832586896310057No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACCACA4920.1180858570585101No Hit
AGCACCAGCCCTGGGGGAGAAGAGGCTCAGAACCTGCTAGCCCAGTCATC4830.11592574991719588No Hit
TCCTGGGCCTAGGCCGCCTCCTTCATCCCTGCTGTGCCCAGCCTCTCCCA4800.1152057142034245No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTATTACTACA4610.1106454880162056No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCCCCCCATGATGGTACTACTACA4570.10968544039784373No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAAACACCCCATGATGGTACTACTACA4560.10944542849325326No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTAATACTACA4490.10776534516111999No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATTGTACTACTACA4360.10464519040144392No Hit
CCAACATGCGGCCTTTACCCTGGGGAGGTGGGGCGGGAACATTCCCTGGA4240.10176504754635829No Hit
CGGCAGCAGCACATGGCACCCGCGCAGAGGCAGTGCAGGCAGCCCGGGCC4200.10080499992799644No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTTCA4170.10008496421422503No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)