FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718078
Sequences flagged as poor quality0
Sequence length208
%GC61

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTACA21579730.052027774141525No Hit
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC566377.887304721771173No Hit
CACACCAAGCTCAGAGAGGGCAGGGGTGGGAAGGAGACATCAGATGTGGG220523.070975576469409No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA163852.281785544188793No Hit
CCATCATAGCCCTGCTGACCCTGTGACCTTGTGGCAGCCCCTCTGGCCCC136391.8993758338230666No Hit
TGGGGACAGGGACAGGTGGTCCTGGGATCAGGCTTCAGCTGCACGTCCTG115271.6052573675840227No Hit
ATGCAGACACCACACGCTCTCCTGCAGTAACTCCTTTCATCTTCACGGTG65970.91870242508474No Hit
CTGTGGGCTAAGGTCTCCCTTTGGCCAGGCAGGGGTGCTGGAAGGCGGTC54510.7591097345970772No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTACC46750.6510434799562165No Hit
TCTGTGGCTGCTGCACGGCACAGCCGAGGAGAGCTGGGTCCAGGCCTGGC44380.6180387088867783No Hit
CACAGTGTGACACTTCGCAGGGACCAGGCGGCCTGGAGTGGACCCTTCCT35500.49437526285445316No Hit
ATTTTTAGCCCTTGGCTAGGTGTGGGGCACATAGAAGGAGCCCCATCATG34220.47654990126420804No Hit
TCCTGGGCCTAGGCCGCCTCCTTCATCCCTGCTGTGCCCAGCCTCTCCCA32440.4517615078027735No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGCACTACTACA32150.44772294931748363No Hit
CGGCAGCAGCACATGGCACCCGCGCAGAGGCAGTGCAGGCAGCCCGGGCC28890.4023239815173282No Hit
TTGTGGCAGCCCCTCTGGCCCCTTGGCCCCGTCCTGTTACCTCTCCTCGT23150.322388375636073No Hit
CCTGTCCTTCTCACGGGGGGTCTCCAGCACACCCTGCTGCCACTGGCCCC23050.32099576926183504No Hit
CAGGCCAGCCCACCACGTTTGGGACTCTGCACATCCGGACTGGTGTTATC23030.32071724798698753No Hit
CAGGAAGGGGCTCTGGTGCCGGGCTGAGCGGGGGAAGCAGGGGTAGCGGA22600.3147290405777646No Hit
CCAACATGCGGCCTTTACCCTGGGGAGGTGGGGCGGGAACATTCCCTGGA21740.30275262575931866No Hit
AGCACCAGCCCTGGGGGAGAAGAGGCTCAGAACCTGCTAGCCCAGTCATC20680.28799099819239693No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGAACTACTACA19000.2645952111052003No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTCCA16500.2297800517492529No Hit
TGAAACTGCGTGGATCTGCTGTTTCCATGCTCACGGGCCCCTCCCCGCCC16060.22365258370260613No Hit
TTGGGGGAAAGCAAAAAGGGGCAGAGATTTAACTCAGGGTCGAGTATGGG14260.19858566896632399No Hit
GTGGGCAGGGGCTTCCAAGCCAACAGGGCAGGACACACCAGAGGCTGACT13370.18619147223560673No Hit
CCCTCCCCTTCCTCAGACTTATATAGGAAACAGGGCAGGCAACCCCTCTT12890.1795069616392648No Hit
ACCATCATCGCTCCGCCGGGCCACACAGGCGTCGCCTGCTCTTGCCACCA12540.17463283932943216No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATGACC12420.17296171168034669No Hit
CTACCCTGGGGTCTGGGGGATGTGGGCCCGCTCAGGGGCCTGGGGTACAC12030.1675305468208189No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTCCTACA11880.16544163725946207No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTACT11600.16154233941159596No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGGACTACTACA11000.1531867011661686No Hit
ATGTTTCCCTGGCCCCAGGGGTCGAACCAGTTGTCACATTGTGACAACAA10870.15137631287965933No Hit
CCAGGCTGTCCTGCAGGACCAGGGACAAAACGAGCCCTAGGACACGAAGA10250.14274215335938434No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTATA10050.13995694061090858No Hit
CACGCGCAGGATCTCACTCCAGCCACGTCCCCAGCTGGACCCCGACCTCG9920.1381465523243993No Hit
CCACACACCCCCGCAGGACCCTCGGTCTCCACGCTGACCCCACACACCCC9750.13577912148819488No Hit
CAGCCTGGAGTCGCCCATTCCGCCCCCACCAAAGGACCCTGCACCCAGCT9080.12644865878080097No Hit
CGCTTGCAGTATCTCCCAGTTTCACCACTCCAAGTTTGGAGGTTCTCCTT8910.12408122794459656No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCCATCGTTACTGTAGTCATC8890.12380270666974896No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCCTGATGGTACTACTACA8800.12254936093293488No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTAATACA8520.11865006308506874No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTAAA8520.11865006308506874No Hit
CGGCCAGGGCTGGGGGCTGGGGGTGAGTCTGAGAGAGATGGAGGCTCCTT8500.11837154181022118No Hit
CTGCCTAGGATGTCCTATCTTAAGGTGAAACTGTGAGGTTTTGGAAATGT8490.11823228117279738No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGGGCAGGGTGCTTCC8360.11642189288628813No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTATTACA8180.1139152014126599No Hit
AATGTCCCTTGGCCCCAGACATCAAAAGCATCGAGTTCCAGTGCCTCAAT7930.11043368547706517No Hit
GGGGTCCCTTGGCCCCAGACGTCCAAACTGTAATAGTAGGCGGACCGAAC7840.10918033974025106No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTATGATCCATCCCTCGCACA7690.10709143017889422No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGCGTCGAATACACTCCTTAATCT7670.10681290890404663No Hit
CGGAGAGGGACGGGAAAGCTCCTGGTGGTTCTCCAGTTTCACCCACGATG7530.10486325998011357No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGCTGGTACTACTACA7510.10458473870526601No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATACCC7230.1006854408573999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)