FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001132232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398969
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT8873522.241076374354897No Hit
CTTACNTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT345468.658818103662188No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC244206.12077630091561No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT121743.051364893011738No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC118442.9686516997561214No Hit
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG91692.298173542305292No Hit
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC83732.0986592943311386No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG75421.8903724349510864No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT46731.1712689457075611No Hit
CTTACNTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG45801.1479588639718876No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT43391.087553168291271No Hit
CTTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT41931.0509588464266648Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC41171.031909747373856No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT38300.9599743338454867No Hit
CTTACCTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT27660.6932869471061661Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG25610.6419045088716167No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG24160.6055608330471791No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT21210.5316202511974615No Hit
CTTACCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT16500.4135659662780818Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCCCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT14920.37396389193145335Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT12270.30754269128679174No Hit
CTTACNTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT12180.3052868769252749No Hit
CTTCCNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT12140.3042842927646008Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA11970.30002331008173566No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC11660.2922532828365111No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC11040.276713228346062No Hit
ATTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT10250.2569121911727478No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG8510.21329978018342277No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG8510.21329978018342277No Hit
CTTACNTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT7880.19750907965280512Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT7490.18773388408623226No Hit
CTTACCTGAGGAGACGGTGACCTGCTGTCTCTTTTCGGAGCGGGACGCCG7290.18272096328286158No Hit
CTTACNTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT7120.1784599805999965No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC6760.16943672315392924No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG6580.16492509443089565No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT6260.15690442114550252No Hit
CTTACCTGAGGAGACGGTGACCACAGGCGGAGAACACTGACAGCATCCTT6010.15063827014128917No Hit
CTTACCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT5900.1478811636994353Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG5810.14562534933791849No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA5690.14261759685589606No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC5660.14186565873539048No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA5630.14111372061488486No Hit
CTTACCTGAGGAGACGGTGACCGAGGTCGAGGGGCTGGCGGAAGGGACAG5540.13885790625336805No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC4870.12206462156207624No Hit
CTTACGTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT4590.1150465324373573No Hit
CTTACCTGAGGAGACGGTGACCAGATCGGAAGAGCACACGTCTGAACTCC4530.11354265619634607Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG4420.1107855497544922No Hit
CTTACNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT4360.10928167351348099Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCACAGGTGGATAACACTGACAGCATCCTT4350.10903102747331246No Hit
CTTACCTGAGGAGACGGTGACCACGAAGGCGGCAAAGGCGACGGAATGGA4320.10827908935280685No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT4210.10552198291095298No Hit
CTTCCNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT4170.10451939875027884Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA4080.10226358438876204No Hit
CTTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATACTT4000.10025841606741376No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC3990.10000777002724523No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)