FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608924
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT10538117.306100597118853No Hit
CTTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT8908614.630068777055921Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT416486.839605599385144No Hit
CTTCCNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT263344.324677628078381Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC204293.3549342775124646No Hit
CTTACCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT181522.98099598636283Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCCCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT139212.2861637905551433Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG115861.902700501211974No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT101741.6708160624314363No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC82431.3536993122294407No Hit
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC70861.1636920206790995No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG64001.0510342834245325No Hit
CTTACCTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT59920.9840308478562185Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG56880.9341067193935533No Hit
CTTACNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT50020.8214489821389861Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCCNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT39930.6557468583928372Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT38140.6263507432783073No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT35150.5772477353495674No Hit
CTTACCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT33980.5580335148557127Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCCCTGGGGGCGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC31310.5141856783440955Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC30470.5003908533741485No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT29690.487581373044912No Hit
CTTTCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT26350.4327305213786942Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCCCTGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC23400.38428440987709467Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACCTGAGGAGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC22440.3685188956257267Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACNTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG22360.367205102771446No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT20410.3351814019483548No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG17920.2942895993588691No Hit
CTTACNTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT16440.2699844315546768Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCCCTGGGGTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC14020.2302421977126866Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCAGATCGGAAGAGCACACGTCTGAACTCC13050.21431245935453358Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT12580.20659392633563464Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT12440.2042947888406435No Hit
CTTACNTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT10420.1711215192700567No Hit
CTTACCTGAGGAGACGGTGGACCAGATCGGAAGAGCACACGTCTGAACTC10340.16980772641577602Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT10100.16586634785293403Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT9870.1620891933968771No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC9740.159954280008671No Hit
CTTCCCTGGGGGTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC9250.15190729877620196Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA8990.1476374719997898No Hit
CTTACNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT8760.14386031754373288Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCCNTGGGGGCGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC8690.1427107487962373Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACCTGAGGAGACGGTGACCTGCTGTCTCTTTTCGGAGCGGGACGCCG7780.12776635507879472No Hit
CTTACCTGAGGAGACGGTGACCACAGGCGGAGAACACTGACAGCATCCTT7210.11840558099204497No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT7110.11676333992419416No Hit
CTTACNTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT7080.1162706676038389No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG6910.11347885778849248No Hit
CTTTCNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT6830.11216506493421181Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC6800.11167239261385659No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG6770.11117972029350133No Hit
CTTCCNTGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC6390.10493920423566816Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACNTGAGGAGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC6350.10428230780852783Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC6320.10378963548817258No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)