FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001132236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548037
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT11637921.235610004434008No Hit
CTTACNTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT459068.376441736598077No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC306695.596155004132933No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT148672.712773042696022No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC137962.5173482812291867No Hit
CTTACCTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT135292.4686289429363346Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG120742.2031359196550597No Hit
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC110792.0215788350056654No Hit
CTTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT100881.8407516280835052Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG91261.6652160346837894No Hit
CTTACNTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG60671.1070420427817829No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT58151.0610597459660571No Hit
CTTACCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT52750.9625262527895015Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT51490.9395351043816385No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC46450.8475705107501865No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT43550.79465437552574No Hit
CTTCCCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT39370.7183821530298137Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT38240.6977631072354604Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG32340.590106142468483No Hit
ATTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT30590.5581739919020067No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG29980.547043356561692No Hit
CTTCCNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT28720.524052208153829Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT26880.49047783270107675No Hit
CTTACCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT19620.35800502520815203Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC18210.3322768353231625No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA16980.30983309521072483No Hit
CTTACNTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT15460.2820977415758425No Hit
CTTACNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT14500.2645806761222326Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT14440.263485859531382No Hit
CTTACCTGAGGAGACGGTGACCTGCTGTCTCTTTTCGGAGCGGGACGCCG12590.2297290146468213No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC12560.22918160635139595No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT11490.2096573771478933No Hit
CTTACCTGAGGAGACGGTGACCAGATCGGAAGAGCACACGTCTGAACTCC11370.20746774396619205Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCCNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT11080.20217613044374744Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG10580.1930526588533256No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG10500.1915929033988581No Hit
CTTACNTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT9310.1698790410136542No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT9020.16458742749120953No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC8810.16075556942323235No Hit
CTTACCTGAGGAGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC8040.14670542317398277Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCACAGGCGGAGAACACTGACAGCATCCTT7750.14141380965153813No Hit
CTTACCTGAGGAGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAA7690.14031899306068749Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG7440.1357572572654766No Hit
CTTACCTGAGGAGACGGTGACCCCAACATGCGGCCTTTACCCTGGGGAGG6780.1237142747661198No Hit
CTTACCTGAGGAGACGGTGACCGAGGTCGAGGGGCTGGCGGAAGGGACAG6770.12353180533431138No Hit
ATTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC6400.11678043635739922No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC6370.11623302806197391No Hit
CTTACCTGAGGGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAAC6290.11477327260750643Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTACCTGAGGAGACGGTGACCACAGGTGGATAACACTGACAGCATCCTT6250.11404339488027268No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA6170.11258363942580518No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC6130.11185376169857145No Hit
CTTACCTGAGGAGACTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT6120.11167129226676302Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC5960.10875178135782804No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA5850.10674461760793524No Hit
CTTACGTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT5750.10491992328985086No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT5690.10382510669900025No Hit
CTTACNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT5560.10145300408549059Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)