FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001132239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457959
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT9413220.55467847558406No Hit
CTTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT5436711.871586757766526Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT379538.287423109929055No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC161923.5356876925663654No Hit
CTTCCNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT158753.4664675222017696Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG94422.061756620134117No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC93822.0486550106013857No Hit
CTTCCCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT91662.0014892162835536Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT85451.8658875576197869Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT79081.7267921364139585No Hit
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC56911.2426876641795446No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG48671.0627588932633707No Hit
CTTACNTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG47051.0273845475249967No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT41000.8952766514032916No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC34330.7496304254310976No Hit
CTTCCNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT27680.6044209197766612Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT26700.5830216242065337No Hit
GTTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT23770.5190420976550303No Hit
CTTACNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT23200.5065955685989357Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT22100.48257595112226204No Hit
CTTCCCTGGGGGCGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC20740.45287896951473827Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT17500.3821302780379903Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG16290.35570869881364925No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT16010.3495946143650414No Hit
CTTCCCTGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC14960.326666797682762Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG13520.29522293480420736No Hit
CTTACCTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT13160.2873619690845687Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT12520.27338691891632216Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC12150.2653075930378047Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACNTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT10860.23713913254243285No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC9960.21748671824333618No Hit
CTTCCCTGGGGTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC8420.18385925377599305Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT7840.17119436456101964Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT7570.16529864027129065No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA7410.16180487772922905No Hit
CTTACCTGAGGAGACGGTGACCACAGGCGGAGAACACTGACAGCATCCTT6720.1467380267665883No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC6190.13516493834600915No Hit
ATTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT6070.13254461643946294No Hit
CTTCCNTGGGGGCGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC5790.12643053199085508Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACNTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT5720.12490201087870312No Hit
CTTACCTGAGGAGACGGTGACCAGATCGGAAGAGCACACGTCTGAACTCC5660.12359184992543001Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCCCTGGGGGTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC5620.12271840928991459Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACCTGAGGAGACGGTGACCTGCTGTCTCTTTTCGGAGCGGGACGCCG5440.11878792643009527No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC5340.11660432484130676No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG5100.11136368102821431No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG5020.10961679975718351No Hit
CTTACGTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT4970.10852499896278923No Hit
CTTACCTGAGGAGACGGTGACCACAGGTGGATAACACTGACAGCATCCTT4720.10306599499081796No Hit
CTTACCTGAGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT4670.10197419419642369Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)