FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001132240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500618
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT10268820.512246862877483No Hit
CTTACNTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT400327.996516305845974No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC346096.913255216552342No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC188113.7575556612027534No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT169513.386014885601397No Hit
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC117992.356886887806671No Hit
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG116022.317535526089753No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG97671.950988578117447No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT83211.6621455880531661No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC67361.345536916371365No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT57501.1485803546816136No Hit
CTTACNTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG57311.1447850456835351No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT47650.9518235460970241No Hit
CTTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT42740.8537447714624724Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG33200.6631803091379056No Hit
CTTACCTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT32890.656987962877883Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG32340.6460015420939719No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT31760.6344158619945748No Hit
CTTACNTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT22440.44824596798357225No Hit
CTTACCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT17460.3487689216128865Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA17210.343775093983836No Hit
CTTCCCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT16310.3257973145192542Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT16200.323600030362472No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC14390.28744471832814644No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC12550.2506901469783348No Hit
CTTCCNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT12090.24150150414088187Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG11560.23091458956729485No Hit
ATTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT10950.2187296501524116No Hit
CTTACNTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT10800.21573335357498136No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC9520.1901649561142428No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG9250.18477162227486826No Hit
CTTACNTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT8960.1789787822251697Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT8920.1781797698045216No Hit
CTTACCTGAGGAGACGGTGACCTGCTGTCTCTTTTCGGAGCGGGACGCCG8410.1679923614412586No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC7510.15001458197667683No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG7360.14701828539924652No Hit
CTTACCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT7150.1428234701908441Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCACAGGCGGAGAACACTGACAGCATCCTT7090.141624951559872No Hit
CTTACCTGAGGAGACGGTGACCGAGGTCGAGGGGCTGGCGGAAGGGACAG7070.14122544534954795No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG6930.1384289018772797No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT6240.12464593762110032No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA6200.12384692520045223No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG6160.12304791277980417No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA5900.11785433204559166No Hit
CTTACCTGAGGAGACGGTGACCACGAAGGCGGCAAAGGCGACGGAATGGA5700.11385926994235125No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC5550.11086297336492096No Hit
CTTCCNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT5400.10786667678749068Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC5150.10287284915844017No Hit
CTTACGTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT5100.10187408363263008No Hit
CTTACNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT5100.10187408363263008Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)