FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001132263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708010
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGAAGCGAACTGATTGGTTAGTTCAAATAAGGCACAGGGTCATTTC41119458.07742828491123No Hit
AGCGANAAGCGAACTGATTGGTTAGTTCAAATAAGGCACAGGGTCATTTC13249118.71315376901456No Hit
AGCGATAAGCGAACTGATTGGTTAGTTCAAATAAGGCACAGGGTCATTTC418665.913193316478581No Hit
AGCTATAAGCGAACTGATTGGTTAGTTCAAATAAGGCACAGGGTCATTTC116051.6391011426392283No Hit
AGCTAGAAGCGAACTGATTGGTTAGTTCAAATAAGGCACAGGGTCATTTC93431.31961412974393No Hit
AGCTANAAGCGAACTGATTGGTTAGTTCAAATAAGGCACAGGGTCATTTC47570.6718831654920129No Hit
AGCGAGAAGCGAACTGATTATGCGCACCCTGGAAACATCTGAAGATCGGA45380.6409513989915396No Hit
AGCGAGAAGCGAACTGATTGGCTAGTTCAAATAAGGCACAGGGTCATTTC34000.4802192059434189No Hit
AGCGAGAAGCGAACTGATTGGGTAGTTCAAATAAGGCACAGGGTCATTTC19020.2686402734424655No Hit
AGCGAGAAGCGAACTGATTGCACCCTGGAAACATCTGAAGATCGGAAGAG16460.23248259205378455No Hit
AGCGAGAAGCGAACTGGAAACATCTGAAGATCGGAAGAGCACACGTCTGA16380.23135266451038827Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGANAAGCGAACTGATTATGCGCACCCTGGAAACATCTGAAGATCGGA14240.20112710272453782No Hit
AGCGAGAAGCGAACTGATTGGTTAGTTCACATAAGGCACAGGGTCATTTC13090.18488439428821626No Hit
AGCAAGAAGCGAACTGATTGGTTAGTTCAAATAAGGCACAGGGTCATTTC13090.18488439428821626No Hit
AGCGAGAAGCGAACTGATTGGAAAGAGTGTGCACCCTGGAAACATCTGAA13020.18389570768774452No Hit
AGCGAGAAACATCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC11020.15564751910283753Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGAGAAGCGAACTTATTGGTTAGTTCAAATAAGGCACAGGGTCATTTC10140.14321831612547845No Hit
AGCGAGAAGCGAACTGTGAACACCCAACTCCAGTGGCCAGAATGTCAGAC9210.1300829084334967No Hit
AGCGAGAAGCGAACTGATTATGCACCCTGGAAACATCTGAAGATCGGAAG8790.12415078883066624No Hit
AGCGAGAAGCGAACTAATTGGTTAGTTCAAATAAGGCACAGGGTCATTTC8620.12174969280094913No Hit
AGCGAGAAGCGAACTATTATGCGCACCCTGGAAACATCTGAAGATCGGAA7100.10028106947641982No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)