FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001132268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC1813.432835820895523No Hit
CTTANCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC75.223880597014925No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC75.223880597014925No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT64.477611940298507No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT53.731343283582089No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC53.731343283582089No Hit
CTTANCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG53.731343283582089No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT42.9850746268656714No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC32.2388059701492535No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC32.2388059701492535No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG32.2388059701492535No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA21.4925373134328357No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG21.4925373134328357No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAGGGACAAC10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCCTTCTGCGGGGCTCTGGACAAGCAGCAG10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATCGT10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGGTAC10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGGCAAGTT10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTAGGC10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTTGAG10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAAT10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGCGT10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGGTCC10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGACACCTG10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATCA10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGCCCATTGTCCCTTGGCCCCAGATATCAAAAG10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGTAAATCTG10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAAC10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCAGGATTCCCTGGCCCCAGCGTTTATAGA10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCAAATA10.7462686567164178No Hit
CCTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGACCCCAGACATCCATTC10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCCAAAG10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCGATAC10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGAATTCCCTGACCCCAGAGGTCGAACC10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCAATAT10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGAACC10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAAGGGTCGAGTC10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA10.7462686567164178No Hit
TTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATACC10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCTTGGCCCCAGTAGTCAGGGA10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAA10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATTT10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAC10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGGTCGAACC10.7462686567164178No Hit
CTTACCTGAGGAGATGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACG10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATTAA10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCATTGTCCCTCGGCCCCAGATATCAAAAG10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAAG10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCTCGG10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGTACC10.7462686567164178No Hit
CTTACCTGAGAAGACGGTGACCAGGGCTCCCTGGCCCCAGTAGTCAAAGT10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGGTA10.7462686567164178No Hit
ATTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC10.7462686567164178No Hit
CTTACTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGTA10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGC10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGTCG10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTGAAGAC10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCCAAT10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTATTCTTTAC10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATCCA10.7462686567164178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCTGGGTTCCCTGGCCCCAGTAGTCAAACG10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAAGGGA10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACTAGTCCACTC10.7462686567164178No Hit
CTTANCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGCT10.7462686567164178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)