FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001132294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201578
Sequences flagged as poor quality0
Sequence length300
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC188821985.76661830741405No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC177640.8068757954521711No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC136500.620009829313338No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC86940.394898568208803No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCCTAC60720.2758021746220211No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATAC60380.2742578277944275No Hit
CTTACCTGAGGAGACGGTGACCGTGGCCCCTTGGCCCCAGACGTCCATAC59100.2684438162081925No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGGCGTCCATAC55490.2520464866563892No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGC48390.21979689113899212No Hit
CTTACCTGAGGAGACGGTGCCCGTGGTCCCTTGGCCCCAGACGTCCATAC45900.20848682172514443No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCAGGCTG44000.19985664827682686No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCAATAC42910.19490565403542368No Hit
CTTACCTGAGGAGACGGTGACCGCGGTCCCTTGGCCCCAGACGTCCATAC41840.19004550372505538No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG39950.18146075224225533No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTCGGCCCCAGACGTCCATAC39240.17823579269051562No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG39000.17714566551809657No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCGTAC38610.1753742088629156No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGCCCATAC34460.15652409317316943No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATCC32370.14703090238002015No Hit
CTTACCTGAGGAGACGGTGACCGTGGTTCCTTGGCCCCAGACGTCCATAC28890.13122405837994383No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCGGACGTCCATAC28670.1302247751385597No Hit
CTTACCTGAGGAGACGATGACCGTGGTCCCTTGGCCCCAGACGTCCATAC27700.12581884448336603No Hit
CTTACCTGAGGAGACGGTGACCATGGTCCCTTGGCCCCAGACGTCCATAC27620.12545546875922634No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT26750.12150375775920727No Hit
CTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACC23630.10733210451775953No Hit
CTTACCTGAGGAGACGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACC23190.10533353803499126No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)