FastQCFastQC Report
Wed 8 May 2024
EGAF00001132295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510156
Total Bases153 Mbp
Sequences flagged as poor quality0
Sequence length300
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT13770326.99233175734481No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT6005311.771497345909879No Hit
CTTACCTGAGGAGACGGTGACCCCACCTGCCTTTAATTAAAAGCTCAGTC5284210.35800813868699No Hit
CTTACCTGAGGAGACGGTGACCAGGCCTGCTGTCCCCCAGGTAAGGACAG361317.082343440045789No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT127692.502959878938991No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGTAGTCAAAGT48870.9579422764801355No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT48390.9485333897866535No Hit
CTTACCTGAGGAGACGGTGACCTGGTGATCGACATCCCGGAGTGACCGTG42160.8264138812441684No Hit
CTTACCTGAGGAGACGGTGACCCTTTCCCCCTCCTGGAGATGCTCTGCAT41070.8050478677110531No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGCCTGCACTCGGGAGGGGAGAG29330.574922180666306No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT28180.5523800562965053No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGCCTCCACTCGGGAGGGGAGAG23860.46770007605516745No Hit
CTTACCTGAGGAGACGGTGACCTGGTGACCTGGCATGTGAGGCCCACGGG23200.4547628568516297No Hit
CTTACCTGAGGAGACGGTGACCAGGGGCAAGGAGCCGGCTGAGCTCTCCT22030.43182869553626735No Hit
CTTACCTGAGGAGACGGTGACCACTTGGGGACCAGAGCTCAGATCCAGAG20830.4083064788025624No Hit
CTTACCTGAGGAGACGGTGANCAGGGTGCCCTGGCCCCAGTGCTGGAAGT20340.3987015736362995No Hit
NTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT19670.38556833595998086No Hit
CTTACCTGAGGAGACGGTGACCCATGGTGATCCCACTGGGGCAGGCACAG19390.3800798187221164No Hit
CTTACCTGAGGAGACGGTGANCCCACCTGCCTTTAATTAAAAGCTCAGTC19200.37635546773927975No Hit
NTTACCTGAGGAGACGGTGACCCCACCTGCCTTTAATTAAAAGCTCAGTC15740.3085330761570971No Hit
CTTACCTGAGGAGACGGTGACCAAGGGAGGCCTCCTTAACCTCCCAAGCC14870.2914794690251609No Hit
CTTACCTGAGGAGACGGTGACCCCACCTGCCTTTCATTAAAAGCTCAGTC14630.28677502567841995No Hit
CTTACCTGAGGAGACGGTGACCCCCACACACATCAGTGCTCCCCACACAC14600.28618697026007733No Hit
CTTACCTGAGGAGACGGTGACCCCCAGGCCTCTCTCCTCTCAAGGGGCTG13410.2628607719991532No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC12190.23894651831988648No Hit
CTTACCTGAGGAGACGGTGANCAGGCCTGCTGTCCCCCAGGTAAGGACAG12130.23777040748320122No Hit
NTTACCTGAGGAGACGGTGACCAGGCCTGCTGTCCCCCAGGTAAGGACAG11640.22816550231693836No Hit
CTTACCTGAGGAGACGGTGACCTGGGGCAGGGGAGGGGAGCAGGGCCGTG11260.2207168003512651No Hit
CTTACCTGAGGAGACGGTGACCCCACCTGCCTTTACTTAAAAGCTCAGTC10930.21424819074949622No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC9800.19209810332525737No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT9300.18229717968621362No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGC8740.17132014521048464No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC8000.15681477822469989No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGCTGGAAGT7780.15250237182352064No Hit
CTTACCTGAGGAGACGGTGACCTCCCGGGCCCTCCAGCATTCTCTGGGTC7280.14270144818447691No Hit
CTTACCTGAGGAGACGGTGACCTCACAGCCCTGCCCGCCAGCCGCCACAC7020.13760496789217416No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGTAGTCAAAGT6710.13152839523596702No Hit
CTTACCTGAGGAGACGGTGACCAGACAGAGGAATGCCATTGAGACTCCCA6340.12427571174307467No Hit
CTTACCTGAGGAGACGGTGACCAGGCCTGCTTTCCCCCAGGTAAGGACAG5290.1036937721010828No Hit
CTTACCTGAGGAGACGGTGACCCACTGTGCTGTGGGAGCCTCCATCCTTC5210.1021256243188358No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)