FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456411
Sequences flagged as poor quality0
Sequence length300
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT7631816.721332307941744No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT6528314.303555348140161No Hit
CTTACCTGAGGAGACGGTGACCCCACCTGCCTTTAATTAAAAGCTCAGTC4634610.154444130400012No Hit
CTTACCTGAGGAGACGGTGACCAGGCCTGCTGTCCCCCAGGTAAGGACAG285016.244590949823733No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT180003.9438137994044844No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGCCTCCACTCGGGAGGGGAGAG104522.2900412128542036No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGCCTGCACTCGGGAGGGGAGAG94772.0764179653864607No Hit
CTTACCTGAGGAGACGGTGACCTGGTGACCTGGCATGTGAGGCCCACGGG73191.6035985109911899No Hit
CTTACCTGAGGAGACGGTGACCACTTGGGGACCAGAGCTCAGATCCAGAG51191.1215768243973085No Hit
CTTACCTGAGGAGACGGTGACCTGGTGATCGACATCCCGGAGTGACCGTG47171.0334983162106084No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGTAGTCAAAGT29060.6367068278371907No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT28240.6187405649732368No Hit
CTTACCTGAGGAGACGGTGACCCATGGTGATCCCACTGGGGCAGGCACAG25660.5622125671817726No Hit
CTTACCTGAGGAGACGGTGACCAGGGGCAAGGAGCCGGCTGAGCTCTCCT25030.5484092188838569No Hit
CTTACCTGAGGAGACGGTGANCAGGGTGCCCTGGCCCCAGTGCTGGAAGT23420.5131339954558501No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGCCTCCGCTCGGGAGGGGAGAG23110.506341871690209No Hit
NTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT21250.4655891290963627No Hit
CTTACCTGAGGAGACGGTGACCTGGGGCAGGGGAGGGGAGCAGGGCCGTG16940.3711566986772887No Hit
CTTACCTGAGGAGACGGTGANCCCACCTGCCTTTAATTAAAAGCTCAGTC16930.3709375979106551No Hit
CTTACCTGAGGAGACGGTGACCCTGCAGGCCTCTGTGGCTCCAGCTGCTT16560.36283086954521254No Hit
CTTACCTGAGGAGACGGTGACCTCATGTGTCCTGTGCACCTTGGATGGAG16450.36042076111224314No Hit
CTTACCTGAGGAGACGGTGACCACCTGCTCAGTCATCTGTAAATGCCGGT15760.3453028082145259No Hit
NTTACCTGAGGAGACGGTGACCCCACCTGCCTTTAATTAAAAGCTCAGTC13610.2981961433883057No Hit
CTTACCTGAGGAGACGGTGACCTGGGCTGGGTTTAGGACCTTGCTTGGCA12590.2758478651916803No Hit
CTTACCTGAGGAGACGGTGACCCCACCTGCCTTTCATTAAAAGCTCAGTC12370.2710276483257415No Hit
CTTACCTGAGGAGACGGTGACCCGTTTCCCTGAGGGACTCTTGTCGGTGG12180.26686473375970343No Hit
CTTACCTGAGGAGACGGTGACCCTGGTGCGGAAGGGCTGCTGGACCGGGC11350.24867937012911606No Hit
CTTACCTGAGGAGACGGTGACCAGTGTGCTAGAGTCAAGCCCCACAATGG11100.2432018509632765No Hit
CTTACCTGAGGAGACGGTGACCCCCAGGCCTCTCTCCTCTCAAGGGGCTG10350.22676929346575783No Hit
CTTACCTGAGGAGACGGTGACCCAGTTAGAGATGCTCAGCCCCTGTCGGA9970.21844346433368167No Hit
CTTACCTGAGGAGACGGTGANCAGGCCTGCTGTCCCCCAGGTAAGGACAG9830.21537605360081152No Hit
CTTACCTGAGGAGACGGTGACCTTGGGCAATTTATTTCCTAGAGTCCAAG9660.21165134056804064No Hit
CTTACCTGAGGAGACGGTGACCCCACCTGCCTTTACTTAAAAGCTCAGTC9640.21121313903477348No Hit
CTTACCTGAGGAGACGGTGACCGGGGTTCCCTGGCCCCAGTAGTCAAAGT9330.20442101526913242No Hit
NTTACCTGAGGAGACGGTGACCAGGCCTGCTGTCCCCCAGGTAAGGACAG9290.20354461220259812No Hit
CTTACCTGAGGAGACGGTGACCACGGTGGATGCCAAGGCAGCCGGTGAGG8650.18952216313804882No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGC8290.18163453553923986No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACGTG7700.16870759030785848No Hit
CTTACCTGAGGAGACGGTGACCGCATCTGGCTGTGCCTGACCGTGGCTGT7700.16870759030785848No Hit
CTTACCTGAGGAGACGGTGACCAGGGAGTAGCATGCCCATGTCCATCAGG7300.1599435596425152No Hit
CTTACCTGAGGAGACGGTGACCCCGGGTCAGACGGGCAGAGGGACAGCCC7240.15862895504271368No Hit
CTTACCTGAGGAGACGGTGACCCCATGTCTGATTGGTTCTCGGTGTTATT7220.15819075350944653No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGCTGGAAGT7150.15665704814301146No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGAGATCGAAGT7010.15358963741014128No Hit
NTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT6560.1437301029116301No Hit
CTTACCTGAGGAGACGGTGACCCTAATCCCAGCAAGAATCAGCATGGGGT6420.14066269217875993No Hit
CTTACCTGAGGAGACGGTGANCAGGGTGCCACGGCCCCAGAGATCGAAGT6390.1400053898788592No Hit
CTTACCTGAGGAGACGGTGACCCTTCATGGGCTATGGCACAGGGAGAGCA6120.13408966917975246No Hit
CTTACCTGAGGAGACGGTGACCCGGCCCCTCTTTCCCCCATGCCTTCTTC6100.13365146764648528No Hit
CTTACCTGAGGAGACGGTGACCTGCACGTATACATCCAGATGGCCTGAAG6090.13343236687985172No Hit
CTTACCTGAGGAGACGGTGACCCACTGTGCTGTGGGAGCCTCCATCCTTC5690.1246683362145084No Hit
CTTACCTGAGGAGACGGTGACCTTAAGAACCTATAAATTCTGCAGATGGT5560.12182002624827185No Hit
CTTACCTGAGGAGACGGTGACCTCACAGCCCTGCCCGCCAGCCGCCACAC5200.11393239864946289No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTGAGAACTTGCCCCAGACCACCA5150.11283689481629497No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC5130.1123986932830278No Hit
CTTACCTGAGGAGACGGTGACCTGCCCCATGTCAGGGAAGCCCCTGCGCA5060.11086498791659273No Hit
CTTACCTGAGGAGACGGTGACCTTTGCCAGCACATGGTATTTTCCTTGGT4620.10122455418471508No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)