FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638242
Sequences flagged as poor quality0
Sequence length300
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT26377241.327897568633844No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT6748610.573732220693719No Hit
CTTACCTGAGGAGACGGTGACCCCACCTGCCTTTAATTAAAAGCTCAGTC329205.157918156435961No Hit
CTTACCTGAGGAGACGGTGACCAGGCCTGCTGTCCCCCAGGTAAGGACAG304664.773424500424603No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGCCG197483.094124172335885No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT182942.8663108977472493No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGTAGTCAAAGT91721.4370724584091927No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT90991.4256347905653342No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC68391.0715371285499857No Hit
CTTACCTGAGGAGACGGTGACCTGGTGACCTGGCATGTGAGGCCCACGGG37290.5842611423253249No Hit
CTTACCTGAGGAGACGGTGACCCTTTCCCCCTCCTGGAGATGCTCTGCAT28760.4506127769717443No Hit
CTTACCTGAGGAGACGGTGACCTGGTGATCGACATCCCGGAGTGACCGTG26860.4208435045014274No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC24020.37634627617737476No Hit
CTTACCTGAGGAGACGGTGANCAGGGTGCCCTGGCCCCAGTGCTGGAAGT22860.3581713519323391No Hit
NTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT21020.3293421617505586No Hit
CTTACCTGAGGAGACGGTGACCACTTGGGGACCAGAGCTCAGATCCAGAG17820.27920443969528796No Hit
CTTACCTGAGGAGACGGTGACCCACTGTGTGTGATTCCCACACTGAGCTC17620.2760708320668336No Hit
CTTACCTGAGGAGACGGTGACCCCCAGGCCTCTCTCCTCTCAAGGGGCTG16740.2622829585016342No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAACCAG15910.24927848684354836No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT14340.22467966696018124No Hit
CTTACCTGAGGAGACGGTGACCCATGGTGATCCCACTGGGGCAGGCACAG13020.20399785661238215No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGCCTCCACTCGGGAGGGGAGAG11870.18597961274876929No Hit
CTTACCTGAGGAGACGGTGACCCCAAGGAAGCTGCAGGCTCTGCTCCTGG11580.18143588168751038No Hit
CTTACCTGAGGAGACGGTGACCCCACCTGCCTTTCATTAAAAGCTCAGTC11000.17234841956499258No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGCCTGCACTCGGGAGGGGAGAG10980.17203505880214714No Hit
CTTACCTGAGGAGACGGTGANCAGGCCTGCTGTCCCCCAGGTAAGGACAG10960.1717216980393017No Hit
CTTACCTGAGGAGACGGTGACCAGGTGGGGGGGTCTGTGACACCCGAAGC10720.1679613688851564No Hit
CTTACCTGAGGAGACGGTGANCCCACCTGCCTTTAATTAAAAGCTCAGTC10700.16764800812231098No Hit
CTTACCTGAGGAGACGGTGACCGCCACTTCCCTAGGGACCCACCTTCTGT10590.16592452392666104No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC10060.15762046371125685No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGTAGTCAAAGT9480.14853300158873908No Hit
NTTACCTGAGGAGACGGTGACCAGGCCTGCTGTCCCCCAGGTAAGGACAG9350.1464961566302437No Hit
NTTACCTGAGGAGACGGTGACCCCACCTGCCTTTAATTAAAAGCTCAGTC9080.14226578633183024No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGC8930.13991558061048945No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGCTGGAAGT8620.1350584887863851No Hit
CTTACCTGAGGAGACGGTGCCCAGGGTTCCCTGGCCCCAGTAGTCAAAGT8560.13411840649784876No Hit
CTTACCTGAGGAGACGGTGACCAAGGGAGGCCTCCTTAACCTCCCAAGCC8030.1258143462824446No Hit
CTTACCTGAGGAGACGGTGACCAGGGTCCCCTGGCCCCAGTAGTCAAAGT7990.1251876247567537No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT7420.11625684301565864No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCAAAGT7230.11327991576862695No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGAAGT6940.10873618470736805No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGAGATCGAAGT6790.10638597898602724No Hit
CTTACCTGAGGAGACGGTGACCGGGGTTCCCTGGCCCCAGTAGTCAAAGT6700.10497585555322277No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGTAGTCAGCCG6560.10278233021330466No Hit
CTTACCTGAGGAGACGGTGACCCCACCTGCCTTTACTTAAAAGCTCAGTC6530.1023122890690365No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGCCG6400.10027544411054114No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)