FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536034
Sequences flagged as poor quality0
Sequence length300
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT20684638.58822388132096No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT509379.502568866900234No Hit
CTTACCTGAGGAGACGGTGACCAGGCCTGCTGTCCCCCAGGTAAGGACAG224384.18592850453516No Hit
CTTACCTGAGGAGACGGTGACCCCACCTGCCTTTAATTAAAAGCTCAGTC162703.0352552263475827No Hit
CTTACCTGAGGAGACGGTGACCTGGTGACCTGGCATGTGAGGCCCACGGG127212.3731703585966564No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT126592.3616039281090377No Hit
CTTACCTGAGGAGACGGTGACCCTTTCCCCCTCCTGGAGATGCTCTGCAT102831.9183484629706327No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGTAGTCAAAGT73401.369316125469653No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT70331.3120436390228978No Hit
CTTACCTGAGGAGACGGTGACCCATGGTGATCCCACTGGGGCAGGCACAG38530.7187976882063452No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGCCTGCACTCGGGAGGGGAGAG37850.706111925736054No Hit
CTTACCTGAGGAGACGGTGACCTGGTGATCGACATCCCGGAGTGACCGTG35610.6643235317162717No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC26880.501460728237388No Hit
CTTACCTGAGGAGACGGTGACCACTTGGGGACCAGAGCTCAGATCCAGAG25310.4721715413574512No Hit
CTTACCTGAGGAGACGGTGACCCCCAGGCCTCTCTCCTCTCAAGGGGCTG23230.43336803262479623No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAA22430.41844360618915966No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC21610.4031460690926322No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGCCTCCACTCGGGAGGGGAGAG20610.38449053604808653No Hit
CTTACCTGAGGAGACGGTGANCAGGGTGCCCTGGCCCCAGTGCTGGAAGT18290.34120969938474055No Hit
NTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT16960.31639784043549474No Hit
CTTACCTGAGGAGACGGTGACCCACTGTGCTGTGGGAGCCTCCATCCTTC16010.29867508404317633No Hit
CTTACCTGAGGAGACGGTGACCTCCTCCTGGCCTCCCACACCTTGACCAC11590.21621762798628447No Hit
CTTACCTGAGGAGACGGTGACCTGCCCCATGTCAGGGAAGCCCCTGCGCA11310.21099407873381165No Hit
CTTACCTGAGGAGACGGTGACCCCCACACACATCAGTGCTCCCCACACAC10940.20409153150732975No Hit
CTTACCTGAGGAGACGGTGACCCGAAGAGACAGCCTGAGGTCCGTCCTCA10870.20278564419421155No Hit
CTTACCTGAGGAGACGGTGACCTGGGGCAGGGGAGGGGAGCAGGGCCGTG9900.18468977714100226No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT9300.17349645731427485No Hit
CTTACCTGAGGAGACGGTGACCAGTGATGGCTCCCGCAGGAGCAGCGGGG8940.1667804654182384No Hit
CTTACCTGAGGAGACGGTGACCCACAGCTGGTCTCAAGGTCCACACTGCC8720.16267624814843834No Hit
CTTACCTGAGGAGACGGTGACCCTAAACAACAAATCCCAAAGCTTACATT8610.16062413951353832No Hit
CTTACCTGAGGAGACGGTGACCACCTGCTCAGTCATCTGTAAATGCCGGT8310.15502747960017463No Hit
CTTACCTGAGGAGACGGTGACCTCACAGCCCTGCCCGCCAGCCGCCACAC8210.15316192629572004No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGTAGTCAAAGT7620.1421551617994381No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGC7440.13879716585141988No Hit
CTTACCTGAGGAGACGGTGANCAGGCCTGCTGTCCCCCAGGTAAGGACAG7330.13674505721651986No Hit
NTTACCTGAGGAGACGGTGACCAGGCCTGCTGTCCCCCAGGTAAGGACAG7160.13357361659894706No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGAAGT6820.12723073536380156No Hit
CTTACCTGAGGAGACGGTGCCCAGGGTTCCCTGGCCCCAGTAGTCAAAGT6780.12648451404201974No Hit
CTTACCTGAGGAGACGGTGACCTCTTATAGCTCCTCGTGACCAACTGAGC6760.1261114033811288No Hit
CTTACCTGAGGAGACGGTGACCCCGAGGGACAGTGGTGACCCGGGGACAA6470.12070129879821055No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGCCTCCGCTCGGGAGGGGAGAG6100.11379875157172867No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGCTGGAAGT6090.11361219624128321No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC6060.11305253024994683No Hit
CTTACCTGAGGAGACGGTGANCCCACCTGCCTTTAATTAAAAGCTCAGTC6030.11249286425861046No Hit
CTTACCTGAGGAGACGGTGACCACATCTCTTAGACCAAAATCCATTTAGC5950.11100042161504681No Hit
CTTACCTGAGGAGACGGTGACCTTGCACAGGTGGCAGAACTGATTCTGGA5910.11025420029326498No Hit
CTTACCTGAGGAGACGGTGACCCTGGGCGGGAGCCTGGATGAAAACTGTC5640.10521720637123765No Hit
NTTACCTGAGGAGACGGTGACCCCACCTGCCTTTAATTAAAAGCTCAGTC5530.10316509773633763No Hit
CTTACCTGAGGAGACGGTGACCCCACCGCCACCACCCACCTGGGACCCTG5530.10316509773633763No Hit
CTTACCTGAGGAGACGGTGACCCCAAGACACACAGTGACAAAGACACAGG5530.10316509773633763No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT5490.10241887641455578No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)