FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001132302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084111
Sequences flagged as poor quality0
Sequence length300
%GC54

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC76160970.25193914645271No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGGGGTCGAACC259982.398093922116831No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC252102.3254076381477544No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC185331.7095112954300804No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCAGGCTG78010.7195757629984384No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTCCCCCCAACCTAGCAGCT68660.633329981892998No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG65760.6065799535287438No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCGAACC56410.5203341724233035No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC49810.4594547975253456No Hit
CTTACCTGAGGAGACGGTGACCAGGCTAGGGCCCTTGGTACGGGCTGAGG39800.3671210789301096No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCGAACC34940.3222917210507042No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT33410.30817877505163216No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCAAACC23590.21759764452164032No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG22750.20984936044371844No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACT22730.20966487748948215No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGGGGTCGAACC20620.19020192581755926No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAGCC16310.15044584917965043No Hit
CTTACCTGAGGAGACGGTGACCGGGGTTCCCTGGCCCCAGGGGTCGAACC14890.13734755942887766No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGCCGAACC14870.13716307647464143No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGCACC14200.13098289750772754No Hit
CTTACCTGAGGAGACGGTGACCAGGGGTCCCTGGCCCCAGGGGTCGAACC13370.12332685490692374No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAGC13010.1200061617306715No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTTGCCCCAGGGGTCGAACC12940.11936047139084467No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCCGGCCCCAGGGGTCGAACC12870.11871478105101785No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCGGGGGTCGAACC12590.11613201969171054No Hit
CTTACCTGAGGAGACGGTGACCAGGGTCCCCTGGCCCCAGGGGTCGAACC12530.11557857082900183No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGGGGTCGAACC12470.11502512196629312No Hit
CTTACCTGAGGAGACGGTGCCCAGGGTTCCCTGGCCCCAGGGGTCGAACC12350.11391822424087569No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGAACC11610.10709235493413496No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGCGTCGAACC11560.10663114754854439No Hit
CTTACCTGAGGAGACGGTGACCATGGTTCCCTGGCCCCAGGGGTCGAACC11100.10238803960111095No Hit
CTTACCTGAGGAGACGGTGACCGTGGGCAGGGGCTTCCAAGCCAACAGGG11010.1015578663070479No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGCTCGAACC11000.10146562482992978No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAAC10900.1005432100587486No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)