FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001132303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790994
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG473365.984369034404812No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC446535.645175563910725No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT355974.500286980685062No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT284773.6001537306224822No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGCAG240033.0345362923106878No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC217062.744142180598083No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC136951.7313658510684025No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG106341.3443844074670606No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG93831.1862289726597168No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT85821.0849639820276766No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGTAGT76310.9647355100038686No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT71120.8991218643883518No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAA62490.7900186347810476No Hit
CTTACCTGAGGAGACGGTGACCCACAGTGTGACACTTCGCAGGGACCAGG54980.69507480461293No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA49020.6197265718829725No Hit
CTTACCTGAGGAGACGGTGACCCAGGCCAGCCCACCACGTTTGGGACTCT48840.6174509541159604No Hit
CTTACCTGAGGAGACGGTGACCCTGTGGGCTAAGGTCTCCCTTTGGCCAG46090.582684571564386No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC44690.5649853222654028No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT43500.5499409603612669No Hit
CTTACCTGAGGAGACGGTGACCATTTTTAGCCCTTGGCTAGGTGTGGGGC41860.5292075540396007No Hit
CTTACCTGAGGAGACGGTGACCCAGACTCAGGGCTCAGGGTGACTGTCCC41690.5270583594818672No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC40340.5099912262292761No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAGT36350.4595483657271736No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGGGGCTCTGGTGCCGGGCTGAG35350.44690604479932844No Hit
CTTACCTGAGGAGACGGTGACCATGCAGACACCACACGCTCTCCTGCAGT33250.42035717085085345No Hit
CTTACCTGAGGAGACGGTGACCGGGCAGCACAAGGCAAGCATCTCAGAGC30900.39064771667041726No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGCCG29820.37699401006834443No Hit
CTTACCTGAGGAGACGGTGACCTCTGTGGCTGCTGCACGGCACAGCCGAG29620.37446554588277536No Hit
CTTACCTGAGGAGACGGTGACCGACAGCGCTGGGTGAAATCCCGCACCGT29610.3743391226734969No Hit
CTTACCTGAGGAGACGGTGACCTGGGGACAGGGACAGGTGGTCCTGGGAT29270.3700407335580295No Hit
CTTACCTGAGGAGACGGTGACCCCATCATAGCCCTGCTGACCCTGTGACC26850.3394463169126441No Hit
CTTACCTGAGGAGACGGTGACCTAACTGGTTTGTCCCCACTGTCTCCTCT24370.30809336101158796No Hit
CTTACCTGAGGAGACGGTGACCCCCAGGCCTCTCTCCTCTCAAGGGGCTG24320.30746124496519567No Hit
CTTACCTGAGGAGACGGTGACCAAGGTGGTGGCGGTGGACGGCGACTCGG23990.30328927905900677No Hit
CTTACCTGAGGAGACGGTGACCCCTGTCCTTCTCACGGGGGGTCTCCAGC20860.26371881455485124No Hit
CTTACCTGAGGAGACGGTGACCCGCTTGCAGTATCTCCCAGTTTCACCAC19840.25082364720844913No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC19830.25069722399917066No Hit
CTTACCTGAGGAGACGGTGACCTCCTGGGCCTAGGCCGCCTCCTTCATCC19200.24273256181462816No Hit
CTTACCTGAGGAGACGGTGACCCACGGGGGCGCCGGCCTTGCCAGCTCTC18750.23704351739709784No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCAGGCTG18290.23122804977028902No Hit
CTTACCTGAGGAGACGGTGACCCCGGGTGGTGAGACCTTAGGCTGTGAGC17930.22667681423626473No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGTAG17910.22642396781770788No Hit
CTTACCTGAGGAGACGGTGACCCTGTCTGTAAAAAAAAGTTCTTAATTTT17680.22351623400430343No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGAATAATA17250.21808003600533002No Hit
CTTACCTGAGGAGACGGTGACCCACAAGGGCTGGCCCCTGAATGCAGCAG16080.20328852051975108No Hit
CTTACCTGAGGAGACGGTGANCATTGTCCCTTGGCCCCAGATATCAAAAG15250.19279539414963956No Hit
CTTACCTGAGGAGACGGTGACCCCAGCACCCACAGCCTGCCTATGGGGCT15140.1914047388475766No Hit
CTTACCTGAGGAGACGGTGANCGTGGTCCCTTGGCCCCAGACGTCCATAC15130.19127831563829814No Hit
CTTACCTGAGGAGACGGTGACCTTAGGTGATGAGTTATTTAGGCTCTTTG14860.18786488898777995No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCAT14830.18748561935994457No Hit
NTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG14730.18622138726716006No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTACCAGC14490.1831872302444772No Hit
CTTACCTGAGGAGACGGTGACCTAGAGGCACAGGTCCGCCTCCAGCTCCT14270.18040591964035127No Hit
CTTACCTGAGGAGACGGTGACCGTGGGCAGGGGCTTCCAAGCCAACAGGG14120.17850957150117447No Hit
CTTACCTGAGGAGACGGTGACCACGATTGTATGACAATAAAATGGACCTT14110.17838314829189603No Hit
CTTACCTGAGGAGACGGTGACCCCATCCACTTCTCAGGGAACAGGGCATT13900.17572826089704852No Hit
NTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC13280.16789002192178448No Hit
CTTACCTGAGGAGACGGTGACCCGGCAGCAGCACATGGCACCCGCGCAGA13160.16637294341044306No Hit
CTTACCTGAGGAGACGGTGACCTCTGGCGGGTGTAGAAGAGCTCATGCTT12850.16245382392281105No Hit
CTTACCTGAGGAGACGGTGACCGCCGCGGTGGAGGAGCTTTTGGTGGCCG12360.1562590866681669No Hit
CTTACCTGAGGAGACGGTGACCCAAATCCAGAGTCTGTAGCCCCACCCCA12090.15284566001764868No Hit
CTTACCTGAGGAGACGGTGANCGTGGTCCCTTTGCCCCAGACGTCCATGT11870.15006434941352273No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA11550.14601880671661224No Hit
CTTACCTGAGGAGACGGTGACCATGCCACTGGACACCTCTGGTGCTGACT11550.14601880671661224No Hit
CTTACCTGAGGAGACGGTGACCCAGCCTGGAGTCGCCCATTCCGCCCCCA11030.13944479983413274No Hit
NTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT10640.1345142946722731No Hit
CTTACCTGAGGAGACGGTGACCACCATCATCGCTCCGCCGGGCCACACAG10550.13337648578876704No Hit
CTTACCTGAGGAGACGGTGACCACCATCTTCATGGCCTGTTCCCATGAGG10530.13312363937021016No Hit
CTTACCTGAGGAGACGGTGACCCTGCCTAGGATGTCCTATCTTAAGGTGA10260.12971021271969194No Hit
CTTACCTGAGGAGACGGTGACCCAGTTGGCCAGTAGCCCACCCAGATCTT10180.12869882704546431No Hit
CTTACCTGAGGAGACGGTGACCTGAAACTGCGTGGATCTGCTGTTTCCAT10150.12831955741762896No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT10010.12654963248773063No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA9970.1260439396506168No Hit
CTTACCTGAGGAGACGGTGACCCTGTCCTCATGTCCTGGCACCCGTGTTG9870.1247797075578323No Hit
CTTACCTGAGGAGACGGTGACCTCGGACCTCCTTGCTTCCGACCAGATCC9860.12465328434855384No Hit
CTTACCTGAGGAGACGGTGACCCTGGACTCCCACTGTGTGTGCCAGCGGA9830.12427401472071847No Hit
CTTACCTGAGGAGACGGTGACCAGCACCAGCCCTGGGGGAGAAGAGGCTC9760.12338905225576932No Hit
CTTACCTGAGGAGACGGTGACCTGAGCACCCCCTGGTGTTCTGAGCTTCC9680.12237766658154171No Hit
CTTACCTGAGGAGACGGTGACCTTGGGGGAAAGCAAAAAGGGGCAGAGAT9640.12187197374442789No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGTAGTCAAAGT9570.12098701127947872No Hit
CTTACCTGAGGAGACGGTGACCAGTGATGGCTCCCGCAGGAGCAGCGGGG9420.11909066314030195No Hit
CTTACCTGAGGAGACGGTGACCTGGCCTTGGAAGGACGGGCTGTATCTGG9360.11833212388463123No Hit
CTTACCTGAGGAGACGGTGACCCCAACATGCGGCCTTTACCCTGGGGAGG9270.11719431500112516No Hit
CTTACCTGAGGAGACGGTGACCATGGGAGGGGGCTGCAGAGAGTCTCAGA9170.11593008290834064No Hit
CTTACCTGAGGAGACGGTGACCCCCTCCCCTTCCTCAGACTTATATAGGA9120.11529796686194839No Hit
CTTACCTGAGGAGACGGTGACCTTCTTTGGATTTCAGTTCCCTGCCTCGG8750.11062030811864565No Hit
CTTACCTGAGGAGACGGTGACCCCCCCAAGCTATCCTGAGTAGGAGAGCA8740.11049388490936721No Hit
CTTACCTGAGGAGACGGTGACCAGGAGCAGACAGGGGAGGGGCTGCTGGG8710.11011461528153185No Hit
CTTACCTGAGGAGACGGTGACCTCTCTCCAGGCTCTAAGGGAGAGCGGGT8630.10910322960730422No Hit
CTTACCTGAGGAGACGGTGACCCTTCTGCGGGGCTCTGGACAAGCAGCAG8550.10809184393307661No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGCAG8400.10619549579389982No Hit
CTTACCTGAGGAGACGGTGACCCAGGGACACCACACTCGGTGTCAGCCTC8140.10290849235266007No Hit
CTTACCTGAGGAGACGGTGACCAGGCTAGGGCCCTTGGTACGGGCTGAGG8050.101770683469154No Hit
CTTACCTGAGGAGACGGTGACCCTGGAAATGGGGCCGACCTGATGTGGAC7920.10012718174853413No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGTAGTCAGCAG7920.10012718174853413No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)