FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001132304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777244
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT12775016.43627998414912No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG716879.223229770831297No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG687788.848958628178538No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC501096.447010205289459No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG79401.0215582236723604No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT71220.9163145678834447No Hit
CTTACCTGAGGAGACGGTGANCAGGGTGCCACGGCCCCAGAGATCGAAGT44720.5753662942396468No Hit
CTTACCTGAGGAGACGGTGACCCTGTGGGCTAAGGTCTCCCTTTGGCCAG43280.5568392937095686No Hit
CTTACCTGAGGAGACGGTGACCCACAGTGTGACACTTCGCAGGGACCAGG40980.5272475567518051No Hit
NTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT40830.5253176608632554No Hit
CTTACCTGAGGAGACGGTGACCCAGGCCAGCCCACCACGTTTGGGACTCT40080.5156681814205063No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGGGGCTCTGGTGCCGGGCTGAG39330.5060187019777572No Hit
CTTACCTGAGGAGACGGTGACCATGCAGACACCACACGCTCTCCTGCAGT37220.4788714998121568No Hit
CTTACCTGAGGAGACGGTGACCTAACTGGTTTGTCCCCACTGTCTCCTCT36660.4716665551615709No Hit
CTTACCTGAGGAGACGGTGACCAAGGTGGTGGCGGTGGACGGCGACTCGG34900.44902244340258657No Hit
CTTACCTGAGGAGACGGTGACCCAGACTCAGGGCTCAGGGTGACTGTCCC31970.41132514371291384No Hit
CTTACCTGAGGAGACGGTGACCATTTTTAGCCCTTGGCTAGGTGTGGGGC29110.37452846210456436No Hit
CTTACCTGAGGAGACGGTGACCCCCAGGCCTCTCTCCTCTCAAGGGGCTG27560.35458620458954976No Hit
CTTACCTGAGGAGACGGTGACCTGGGGACAGGGACAGGTGGTCCTGGGAT27200.3499544544570302No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGTAGTCAAAGG24950.3210060161287832No Hit
CTTACCTGAGGAGACGGTGACCTCTGTGGCTGCTGCACGGCACAGCCGAG24400.3139297312041006No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG24230.31174251586374424No Hit
CTTACCTGAGGAGACGGTGANCATTGTCCCTTGGCCCCAGATATCAAAAG23480.30209303642099516No Hit
NTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG23050.2965606682071525No Hit
CTTACCTGAGGAGACGGTGACCCTGTCTGTAAAAAAAAGTTCTTAATTTT22200.28562459150537023No Hit
CTTACCTGAGGAGACGGTGACCCCTGTCCTTCTCACGGGGGGTCTCCAGC21090.2713433619301017No Hit
CTTACCTGAGGAGACGGTGACCCGCTTGCAGTATCTCCCAGTTTCACCAC20880.26864150768613204No Hit
CTTACCTGAGGAGACGGTGACCTCTGGCGGGTGTAGAAGAGCTCATGCTT20660.265810993716259No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC19750.2541029586590569No Hit
CTTACCTGAGGAGACGGTGACCCACGGGGGCGCCGGCCTTGCCAGCTCTC19480.25062914605966724No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCCGGCCCCAGAGATCGAAGT18880.24290956250546802No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGGGGTCGAACC18250.23480399977355887No Hit
CTTACCTGAGGAGACGGTGACCGACAGCGCTGGGTGAAATCCCGCACCGT17520.2254118397826165No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCACGGCCCCAGAGATCGAAGT17420.22412524252358332No Hit
CTTACCTGAGGAGACGGTGACCTGGCCTTGGAAGGACGGGCTGTATCTGG17250.2219380271832269No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC17100.22000813129467708No Hit
CTTACCTGAGGAGACGGTGACCGGGCAGCACAAGGCAAGCATCTCAGAGC17080.21975081184287046No Hit
CTTACCTGAGGAGACGGTGACCCCATCATAGCCCTGCTGACCCTGTGACC16540.2128031866440912No Hit
CTTACCTGAGGAGACGGTGACCAGGCTAGGGCCCTTGGTACGGGCTGAGG14440.185784644204394No Hit
CTTACCTGAGGAGACGGTGACCCAAATCCAGAGTCTGTAGCCCCACCCCA14130.18179619270139105No Hit
CTTACCTGAGGAGACGGTGACCTCCTGGGCCTAGGCCGCCTCCTTCATCC13630.17536320640622508No Hit
CTTACCTGAGGAGACGGTGACCTTAGGTGATGAGTTATTTAGGCTCTTTG13480.17343331051767527No Hit
CTTACCTGAGGAGACGGTGACCTGAGCACCCCCTGGTGTTCTGAGCTTCC13430.17279001188815868No Hit
CTTACCTGAGGAGACGGTGACCTGAGCACCCCCTGGTGTCCTGAGCTTCC12710.16352651162311965No Hit
CTTACCTGAGGAGACGGTGACCTTGGGGGAAAGCAAAAAGGGGCAGAGAT12080.15542094889121047No Hit
CTTACCTGAGGAGACGGTGACCCCGGGTGGTGAGACCTTAGGCTGTGAGC11840.1523331154695308No Hit
CTTACCTGAGGAGACGGTGACCTGAAACTGCGTGGATCTGCTGTTTCCAT11410.14680074725568804No Hit
CTTACCTGAGGAGACGGTGACCACACACTGTCATGCGGGGAGCGTGTAGC11380.1464147680779781No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCAGGCTG11270.14499951109304157No Hit
CTTACCTGAGGAGACGGTGACCGTGGGCAGGGGCTTCCAAGCCAACAGGG11260.14487085136713826No Hit
CTTACCTGAGGAGACGGTGACCCCATCCACTTCTCAGGGAACAGGGCATT11210.14422755273762164No Hit
CTTACCTGAGGAGACGGTGACCAGGAGCAGACAGGGGAGGGGCTGCTGGG11050.14216899712316852No Hit
CTTACCTGAGGAGACGGTGACCCCAGCACCCACAGCCTGCCTATGGGGCT10990.14139703876774862No Hit
CTTACCTGAGGAGACGGTGACCACCATCTTCATGGCCTGTTCCCATGAGG10350.13316281630993612No Hit
CTTACCTGAGGAGACGGTGACCACCAGCCTGGTGCAGGGATGCGGCTGAG9660.12428529522260706No Hit
CTTACCTGAGGAGACGGTGACCCACAAGGGCTGGCCCCTGAATGCAGCAG9600.12351333686718714No Hit
CTTACCTGAGGAGACGGTGACCCCTCTCTCTGGTTCCCAGCACCTGCCTG9590.12338467714128382No Hit
CTTACCTGAGGAGACGGTGACCCCCTCCCCTTCCTCAGACTTATATAGGA9570.12312735768947718No Hit
CTTACCTGAGGAGACGGTGACCCTGTGGTCTCTTCCAACCCCGAGGCTGT9540.12274137851176721No Hit
CTTACCTGAGGAGACGGTGACCCAGCCTGGAGTCGCCCATTCCGCCCCCA8960.11527911440937468No Hit
CTTACCTGAGGAGACGGTGACCCAGGGACACCACACTCGGTGTCAGCCTC8750.11257726016540494No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGC8500.10936076701782195No Hit
CTTACCTGAGGAGACGGTGACCATCCCTGAGTGTGTGCACATTCCACCCT8300.10678757249975555No Hit
CTTACCTGAGGAGACGGTGACCCATCCTGTTTGCAACATGTGCATGGCAA8170.1051149960630124No Hit
CTTACCTGAGGAGACGGTGACCCCCCCAAGCTATCCTGAGTAGGAGAGCA7930.10202716264133271No Hit
CTTACCTGAGGAGACGGTGACCAGCACCAGCCCTGGGGGAGAAGAGGCTC7920.10189850291542939No Hit
CTTACCTGAGGAGACGGTGACCACGATTGTATGACAATAAAATGGACCTT7920.10189850291542939No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)