FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001132313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298471
Sequences flagged as poor quality0
Sequence length300
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC65244450.24709831794472No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT19889015.317246207269935No Hit
CTTACCCGAGGAGACGGTGACCAAGGTTCCCTGGCCCCAGTAGTCAAACC269132.0726685463133174No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC264152.034315745211098No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT178211.3724603783989013No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC168171.2951386669398084No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC157201.2106546853953613No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT156761.2072660844947636No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG110190.8486134846292293No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC100070.7706756639154821No Hit
NTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT82200.6330522591571164No Hit
CTTACCCGAGGAGACGGTGACCAAGGTGCCACGGCCCCAGAGATCGAAGT81920.6308958767658268No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG68240.525541194219971No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCACGGCCCCAGAGATCGAAGT67100.5167616373411497No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAGATCGAAGT42920.33054261512193955No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC41970.3232263177229218No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG32400.24952424813492177No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAACC31100.23951247274679222No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT29700.22873056079034498No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC27890.21479108890379528No Hit
CTTACCTGAGGAGACGGTGACCCAGACTCAGGGCTCAGGGTGACTGTCCC27040.2082449280730952No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCAACC25470.19615378395050792No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCGTCAAACC23850.18367757154376185No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG21070.1622677749445309No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAACC20070.15456640926135432No Hit
CTTACCTGAGGAGACGGTGACCAGGCTAGGGCCCTTGGTACGGGCTGAGG19870.153026136124719No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGC17520.134927926769254No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCC15350.11821596323676078No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTTGCCCCAGTAGTCAAACC13360.10289024552723935No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGTAGTCAAACC13110.10096490410644521No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)