FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396796
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT12005130.255093297311465No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC4823912.157128600086695No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG337328.501093761025817No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT317157.992772104557505No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT295697.451940039718143No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC170084.28633353158802No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT73651.856117501184488No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA38350.9664915976975574No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTATCCCC36610.9226403491970686No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG29990.7558039899595762No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGCTGGAAGT28060.7071643867377696No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC21100.5317593927358139No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT16910.42616357019728024No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGC15100.38054819100999004No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATCCC14990.3777759856450166No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG14310.3606387161160899No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG12180.3069587395034224No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG10830.27293621911511207No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGTAG10830.27293621911511207No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCAG10660.2686519017328804No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAG9400.23689754937045734No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCCA9140.2303450639623383No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC8160.20564723434712043No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCCTGGCCCCAGATATCAAAAG8080.20363108499077612No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCC7980.2011108982953457No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTCGAAGT7760.19556648756539882No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCAAAAG7710.19430639421768364No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAT6860.17288480730652528No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAGT6850.17263278863698223No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA6540.16482020988114798No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA6450.16255204185526062No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAA6410.16154396717708847No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTCCCC6270.15801570580348592No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCGGTT6000.15121120172582386No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGCTCGAAGT5610.14138247361364528No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCTCCATA5580.14062641760501618No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGATC5410.1363421002227845No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC5300.13356989485781107No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT5290.13331787618826801No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATACC5220.13155374550146676No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT5150.12978961481466547No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACA5070.12777346545832116No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGATC4430.11164427060756661No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGAGCTGGAAGT4410.11114023326848053No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTTAACCA4370.11013215859030838No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGGTGGAAGT4260.10735995322533494No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCGAACC4180.10534380386899062No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAACG4030.10156352382584502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)