FastQCFastQC Report
Thu 15 Sep 2022
EGAF00001132317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666627
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG8389912.585598843131166No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT7440111.160814068437071No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC526017.890619491859765No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC514877.723509548818154No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT504437.566900230563718No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG176982.654857964048861No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT96311.4447359617897264No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC81731.2260229483654277No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC72591.0889147904300307No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT68931.0340115236856593No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA49100.7365438243575493No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC45010.675190173815342No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG42470.6370879067304505No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAC34750.5212810162204651No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACA34170.5125804985396631No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAC32710.49067919541212707No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAT27540.41312458091256427No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT27020.4053241167849487No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT26080.39122327778502825No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGCCTGTGA25470.38207273332763303No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCG24940.37412226027448636No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTTTCCCA24610.36917196573196104No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCTAAAG23360.35042085004057744No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCCG23160.34742067152995604No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGCTTCTTAGTGCTTTACA22770.3415703234342443No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCGAACTAACC22380.33571997533853265No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA22220.33331983253003555No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA21990.329869627242821No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCTGTC20610.30916839551953346No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGA20160.30241799387063534No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTTCCAGT19860.29791772610470324No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGG19730.2959676100727993No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGG19710.2956675922217372No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCT19260.2889171905728391No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGA18910.2836668781792517No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG18350.2752663783495118No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCCTGGCCCCAGATATCAAAAG18250.27376628909420114No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCGGGC18230.27346627124313894No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGAC17270.25906541439215636No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGGC17220.258315369764501No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACTAATAACT16920.25381510199856894No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCAGGGG16900.2535150841475068No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGGTA16780.25171497704113394No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAGGTAACTCC16720.25081492348794754No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCC15100.22651347755191434No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGGCTTAAC15090.22636346862638326No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCTTTG14860.22291326333916867No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCAGTA14430.21646287954133273No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTGTGC13620.2043121565733161No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGAGATAAC13250.19876182632866654No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCAGCCGACGA13240.19861181740313547No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCAGGG13220.19831179955207334No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT13150.19726173707335587No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCGTCGC13120.19681171029676267No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAA13080.1962116745946384No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCAATC12970.19456157641379662No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACGAC12820.19231144253083057No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAA12680.19021131757339563No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCAGTCCGAAC12650.1897612907968024No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCAAAG12420.18631108550958783No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACGCA12300.18451097840321498No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGGCA12260.1839109427010907No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCAAGG11990.17986070171175186No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACGGTAGCAGCT11910.1786606303075033No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC11840.17761056782878581No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGCGG11790.17686052320113047No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACTGG11730.17596046964794404No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTCCTCGCCACTTAATCGTA11650.1747603982436955No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGGACAGAG11650.1747603982436955No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTGGGG11610.17416036254157122No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCAGAACCTACCACC11400.17101017510541877No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCCC11400.17101017510541877No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCGCC11350.17026013047776342No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGC11330.1699601126267013No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAGC11310.16966009477563915No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCCCGACCCCCACA11250.16876004122245272No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG11060.16590987163736243No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAACCAC11050.16575986271183135No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCATCGAGGACGAG11020.16530983593523813No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGCCAG10880.16320971097780318No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGACAGGGA10820.16230965742461675No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGACACTATGGA10710.160659559243775No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCAAGGA10700.16050955031824393No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAG10700.16050955031824393No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGGAATAAC10690.16035954139271286No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGCCG10650.15975950569058858No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGCCA10640.15960949676505753No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCGCTGG10620.15930947891399538No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGT10610.1591594699884643No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCAGGC10590.15885945213740216No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGCAAC10570.15855943428634003No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAAGGGT10260.1539091575948769No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTTCGGGCGGGGCGTAGTAA10230.1534591308182837No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCAACTCGGAGTCACCG10220.15330912189275261No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGCCTG10200.15300910404169046No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGACCAAAT10100.1515090147863798No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAC10020.15030894338213124No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCTAGTGGGCTGCCGG9980.14970890768000697No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGTGG9960.14940888982894482No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGACCCCAGTAGTCAAAGA9850.14775879164810307No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCTCGA9800.14700874702044772No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGCACC9790.14685873809491665No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAGAA9730.14595868454173022No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGTTCGGTAACCACTCCAA9630.14445859528641955No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG9550.143258523882171No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCTTGAGTAATAACC9540.1431085149566399No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCACTAG9440.14160842570132923No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAACC9230.13845823826517678No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAACCCAAAG9190.1378582025630525No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAGGT9180.13770819363752143No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCGACCATAGCTGTA9120.13680814008433503No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCTTGT9120.13680814008433503No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCCCCC9110.13665813115880393No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCAGGAAAGCATAGC9060.1359080865311486No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACTC8750.13125780983968546No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTCAAATAAC8570.12855764918012622No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATTA8510.1276575956269398No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACCCGAAAG8460.12690755099928447No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACGC8460.12690755099928447No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGGGATAAT8440.12660753314822232No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGACCTCGG8190.1228573100099456No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGAGGGATA8180.12270730108441452No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTATAA8170.12255729215888346No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACTAGTAAT8160.12240728323335238No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCTAAAGG8150.12225727430782132No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCCGGG8040.12060717612697956No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACTT8030.12045716720144849No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATACCTTGAG7940.11910708687166886No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCTACC7880.11820703331848245No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACCGT7870.11805702439295139No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACCCGGGGT7850.11775700654188925No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTAGGG7770.11655693513764069No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCAATA7740.11610690836104749No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCATTAGTCAATGG7300.10950651563768043No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGACG7160.10740639068024549No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCAGCTG7130.10695636390365226No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGGAAGCCG7050.1057562924994037No Hit
CTTACCTGAGGAGACGGTGACCATTGTTCCCTGGCCCCAGATATCAAAAG6850.10275611398878233No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)