FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001132319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183055
Sequences flagged as poor quality0
Sequence length300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT8655247.28196443691787No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG6597136.038895413946626No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGTCCCAGATATCAAAAG24961.3635246237469614No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCCTGGCCCCAGATATCAAAAG16650.9095626997350522No Hit
CTTACCTGAGGAGACGGTGACCATTGTTCCCTGGCCCCAGATATCAAAAG6310.34470514326295376No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTTGCCCCAGACGTCCATGT6270.34252000764797463No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGC5170.2824287782360493No Hit
CTTACCTGAGGAGACGGTGACCACGGTGGATGCCAAGGCAGCCGGTGAGG5110.2791510748135806No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTTGCCCCAGACGTCCATGT4860.2654939772199612No Hit
CTTACCTGAGGAGACGGTGACCGTGGTTCCTTTGCCCCAGACGTCCATGT4130.22561525224659257No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAATAG3790.20704159951927015No Hit
CTTACCTGAGGAGACGGTGACCATGGTCCCTTTGCCCCAGACGTCCATGT3480.1901067985031821No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCAATGT3250.17754226871705225No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCCAAAG3040.16607030673841194No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAT2940.1606074677009642No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGCCCATGT2720.14858922181857911No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAC2720.14858922181857911No Hit
CTTACCTGAGGAGACGGTGACCGTGGTTCCCTTGCCCCAGACGTCCATGT2700.14749665401108955No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAT2690.14695037010734477No Hit
CTTACCTGAGGAGACGGTGACCATTGTTCCTTGGCCCCAGATATCAAAAG2670.14585780229985523No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATGTCAAAAG2520.1376635437436836No Hit
CTTACCTGAGGAGACGGTGCCCGTGGTCCCTTTGCCCCAGACGTCCATGT2260.12346016224631942No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTTGCCCCAGATATCAAAAG2240.12236759443882987No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAACG1870.10215509000027313No Hit
CTTACCTGAGGAGACGGTGCCCATTGTCCCTTGGCCCCAGATATCAAAAG1860.10160880609652836No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)