FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001132321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351536
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC26312774.8506554094033No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC87912.5007396113058125No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT51651.4692663055846342No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATAC22490.639763779527559No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC21880.6224113604296573No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG21460.6104637931819216No Hit
CTTACCTGAGGAGACGGTGACCATGGTCCCTTGGCCCCAGACGTCCATAC19520.555277411132857No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC13140.3737881753220154No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCAATAC12090.34391925720267624No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC11490.3268513039916253No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG10380.2952755905511811No Hit
CTTACCTGAGGAGACGGTGACCGTGGTTCCTTGGCCCCAGACGTCCATAC10340.2941377270037777No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT8910.2534591051841063No Hit
CTTACCTGAGGAGACGGTGACCGTGGTTCCCTGGCCCCAGACGTCCATAC8060.22927950480178416No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG6930.19713485958763824No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT6740.19173000773747212No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATCC6530.1857562241136043No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAAAC6120.17409312275271951No Hit
CTTACCTGAGGAGACGGTGCCCGTGGTCCCTTGGCCCCAGACGTCCATAC5690.161861089618133No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCCTAC5490.156171771881116No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGGCGTCCATAC4970.14137954576487188No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGC4890.13910381867006508No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG4840.13768148923581086No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGACATCGAAGT4680.13313003504619728No Hit
CTTACCTGAGGAGACGGTGACCGTGGCCCCTTGGCCCCAGACGTCCATAC4570.13000091029083793No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG4430.12601838787492603No Hit
CTTACCTGAGGAGACGGTGACCACGGTTCCCTGGCCCCAGTAGTCGAAGT4220.1200446042510582No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGCCCATAC4040.11492421828774294No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCGTAC3930.1117950935323836No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGAAGTCCATAC3900.11094169587183104No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC3820.10866596877702427No Hit
CTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACC3790.1078125711164717No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTCGGCCCCAGACGTCCATAC3780.10752810522962086No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)