FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399806
Sequences flagged as poor quality0
Sequence length300
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT12613431.548801168566754No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT5600414.007793779983292No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC271146.781789167746358No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT262186.557680475030389No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT143593.591491873558676No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG124553.1152609015372454No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC65401.635793359779493No Hit
CTTACCTGAGGAGACGGTGACCACTAATGGGATTAGCCACTGTCCTTGAT49831.246354481923733No Hit
CTTACCTGAGGAGACGGTGACCAGGCCTGCTGTCCCCCAGGTAAGGACAG38890.9727217700584785No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA36740.9189456886589996No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGCTGGAAGT28240.7063425761494325No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTTATAGC21530.5385111779212918No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGC14770.3694291731489773No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG14670.3669279600606294No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCACGGCCCCAGAGATCGAAGT11720.29314217395436787No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC10330.2583753120263328No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGCCAC9680.24211742695207178No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGTGCTGGAAGT9160.22911111889266292No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA7940.19859631921481918No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCCCCA7720.19309365042045393No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTCGAAGT7230.18083770628754947No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCATGGCCCCAGAGATCGAAGT7010.1753350374931842No Hit
CTTACCTGAGGAGACGGTGACCAAGGGAGGCCTCCTTAACCTCCCAAGCC6990.17483479487551462No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG6940.1735841883313407No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCGGAGAGCAGCTGGTACTAC6830.17083285393415806No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGAGCTGGAAGT6490.16232872943377538No Hit
CTTACCTGAGGAGACGGTGACCCCACCTGCCTTTAATTAAAAGCTCAGTC6380.15957739503659274No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCCGGCCCCAGAGATCGAAGT6370.15932727372775796No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCTGA6280.1570761819482449No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGGTCGAAGT5860.14657108697718393No Hit
CTTACCTGAGGAGACGGTGACCTCCATTTCTGCCCTGCTTCTGCCACTCC5820.1455706017418448No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTATCCTCCCG5790.14482023781534045No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGCA5110.12781198881457506No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCTGG5110.12781198881457506No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGCCTGCACTCGGGAGGGGAGAG4600.11505580206400104No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGGTGGAAGT4510.11280471028448798No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGAGATCGAAGT4440.11105386112264448No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTTCCCCA4420.1105536185049749No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGC4220.10555119232827921No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)