FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001132324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741377
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC612168.25706759179203No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC574577.750038104769907No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT507606.846719010705754No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC421735.688468889647238No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG377305.089178649998584No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT373645.039811054294914No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG213182.8754601235268966No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG101091.363543784066676No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG99131.3371064923783715No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC79641.0742173010492637No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA69050.9313749954476601No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG65120.8783655279297846No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC64810.8741841195505121No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG49010.6610671763488751No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT35250.4754665979656774No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCATTGCCCCAGACGTCCATGT34870.47034100059753675No Hit
CTTACCTGAGGAGACGGTGACCCAGACTCAGGGCTCAGGGTGACTGTCCC33500.4518618732439771No Hit
CTTACCTGAGGAGACGGTGACCGCCGCGGTGGAGGAGCTTTTGGTGGCCG31020.41841060620979614No Hit
CTTACCTGAGGAGACGGTGACCAGGCTAGGGCCCTTGGTACGGGCTGAGG30490.41126174672265253No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT29080.3922430828040255No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACATTTAGCG28530.38482445503434826No Hit
CTTACCTGAGGAGACGGTGACCATGGTCCCTTGGCCCCAGACGTCCATCC28030.3780802479710053No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTTCCATT26360.35555459637943987No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCCAAC25680.3463824747732935No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAATC25300.34125687740515287No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGT24950.33653593246081276No Hit
CTTACCTGAGGAGACGGTGACCCACAGTGTGACACTTCGCAGGGACCAGG24830.3349173227656105No Hit
CTTACCTGAGGAGACGGTGACCATGGTCCCTTGGCCCCAGACGTCCACAG23580.3180568051072531No Hit
CTTACCTGAGGAGACGGTGACCAGGGATCCCTGGCCCCAGTAGTCAAGGC23480.31670796369458454No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGATGC23140.31212190289151137No Hit
CTTACCTGAGGAGACGGTGACCCCATCATAGCCCTGCTGACCCTGTGACC22850.3082102627947724No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCAAGT22850.3082102627947724No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAAC22420.3024102447202975No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCAAGAT21670.2922939341252831No Hit
CTTACCTGAGGAGACGGTGACCAGGTTTCCCTGGCCCCAGTAGTCAAGGT20120.27138689222892No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGAGT19790.26693571556711365No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA19690.26558687415444504No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT19320.26059616092757126No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTCTGCCCCAGAGGTCCATGT19210.2591124353736358No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTGTGCCCCAGACGTCCATGT19080.25735894153716665No Hit
CTTACCTGAGGAGACGGTGACCGTAGTCCCTTGGCCCCAGACGTCCACAC19050.25695428911336604No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGTAGTCAAAAA18870.2545263745705626No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT18600.25088450275635743No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCACAG18510.2496705454849557No Hit
CTTACCTGAGGAGACGGTGACCTGGATTCCCTGGCCCCAGTAGTCGAAGT18350.24751239922468593No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGAAGT18170.2450844846818825No Hit
CTTACCTGAGGAGACGGTGACCTGGGTGCCCTGGCCCCAATACTGGAAGA18160.24494960054061563No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGC18080.24387052741048076No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGG18070.2437356432692139No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGGCGCCCATAC17740.23928446660740754No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAT17560.2368565520646041No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGC17260.23281002782659832No Hit
CTTACCTGAGGAGACGGTGACCAGAGTTCCCTGGCCCCAGTAGTCAAAGT16850.22727977803465713No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCGGTGT16840.22714489389339027No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCGCCC16800.22660535732832282No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAGG16580.22363790622045193No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAT16520.2228286013728508No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGCCCAATAAT16500.2225588330903171No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG16220.21878207713484504No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT16160.21797277228724388No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGGCCGAGC16150.21783788814597702No Hit
CTTACCTGAGGAGACGGTGACCGACAGCGCTGGGTGAAATCCCGCACCGT16000.21581462602697413No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCTGAACCCAA15970.21540997360317357No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGGTTAACCAGTCAAAAT15790.2129820590603701No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCTCCAGTAGTCAAAGT15750.21244252249530268No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCCGGCCCCAGTAGTTCAAGG15400.2077215775509626No Hit
CTTACCTGAGGAGACGGTGACCAGGGCCATAAGGCCACTCCAAAAATCGT15130.20407970573675743No Hit
CTTACCTGAGGAGACGGTGACCCAAATCCAGAGTCTGTAGCCCCACCCCA14700.1982796876622825No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAAA14620.19720061453214763No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTTCAAAA14560.19639130968454646No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCCGGCCCCAGAGGTCGAACC14280.19261455372907443No Hit
CTTACCTGAGGAGACGGTGACCAGTGTTCCCTGGCCCCAGTGGTCGTAGC14190.19140059645767268No Hit
CTTACCTGAGGAGACGGTGACCGCCCTGGGGTGAAAGCAGATTTGCATCA14180.19126571231640582No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTCCCCCACT14090.1900517550450041No Hit
CTTACCTGAGGAGACGGTGACCTGGGTTCCCTGGCCCCAGGGGTCGAACG13870.1870843039371332No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAATCATCCA13530.18249824313406No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCC13340.17993544444998968No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCGAACC12800.1726517008215793No Hit
CTTACCTGAGGAGACGGTGACCATGCCACTGGACACCTCTGGTGCTGACT12700.1713028594089107No Hit
CTTACCTGAGGAGACGGTGACCAGCGTCCCCTGGCCCCAGTAGTCAAAGA12500.16860517658357355No Hit
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG12490.16847029244230668No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTTAGTCA12470.16820052415977296No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAAAC12470.16820052415977296No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAACGTCCTA12460.1680656400185061No Hit
CTTACCTGAGGAGACGGTGACCCCCAGGCCTCTCTCCTCTCAAGGGGCTG11880.16024235982502827No Hit
CTTACCTGAGGAGACGGTGACCACCAGACCACAGGCTCAGAAACGAGGTT11870.16010747568376144No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCGTCAAAAA11700.15781444528222482No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAA11650.15714002457589055No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACTAGTCAAAGT11480.15484699417435394No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCAACCC11440.1543074576092865No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGAAT11420.1540376893267528No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCAAACG11410.1539028051854859No Hit
CTTACCTGAGGAGACGGTGACCGACGTGCTGTAGACGCGCAGGCCGCTCA11400.15376792104421907No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATTT11260.15187954306648305No Hit
CTTACCTGAGGAGACGGTGACCAGGATTCCCTGGCCCCGGAAGGCAAAGG11180.15080046993634816No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTTTAGTT10970.14796790296974413No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACCCACCTC10910.147158598122143No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCAAAG10790.1455399884269407No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCCA10720.1445957994380727No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGATAT10660.14378649459047152No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATAGCAAAAG10470.1412236959064012No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTATTCATTCT10440.14081904348260063No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTATAGAGAAC10420.14054927520006688No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCGGTTGGCG10360.13973997035246574No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAAGA10350.13960508621119888No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGCCCAGAC10320.13920043378739833No Hit
CTTACCTGAGGAGACGGTGACCAGTGTCCCTTGGCCCCAGATGTCATACA10300.1389306655048646No Hit
CTTACCTGAGGAGACGGTGACCAAGCCATCAGGGACCGGTTCTCCCGGCT10210.13771670823346285No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAAATATCAAAAG9900.13353529985419024No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCGTGGCCCCACATATCAAAAG9880.13326553157165652No Hit
CTTACCTGAGGAGACGGTGACCCCAGGCTGTCCTGCAGGACCAGGAACAA9850.13286087914785596No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGTGCG9760.13164692187645424No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGACCTAATG9730.13124226945265366No Hit
CTTACCTGAGGAGACGGTGACCAGGGCATCTGAGCACCGCGGGACCTGCC9710.13097250117011994No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCATAGC9640.1300283121812519No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCGC9510.12827481834478274No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGGCAAAGT9240.12463294653057758No Hit
CTTACCTGAGGAGACGGTGACCCGGCAGCAGCACATGGCACCCGCGCAGA9200.12409340996551012No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGACATCAGAAG9200.12409340996551012No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGG9190.12395852582424327No Hit
CTTACCTGAGGAGACGGTGACCTCCTGGGCCTAGGCCGCCTCCTTCATCC9160.12355387340044269No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCCC9070.12233991612904097No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCATTG9070.12233991612904097No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGCGACC8990.12126084299890609No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGACCGAGT8870.11964223330370377No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGGGGTCGAACC8830.11910269673863635No Hit
CTTACCTGAGGAGACGGTGACCTGGGTTCCCTGGCCCCAGGGGTCGAGCC8550.1153259407831643No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGA8550.1153259407831643No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAA8460.11411198351176258No Hit
CTTACCTGAGGAGACGGTGACCTCTCTCCAGGCTCTAAGGGAGAGCGGGT8250.11127941654515855No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCCTGGCCCCAGATATCAAAAG8180.11033522755629054No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGACATCAAAAC7990.10777242887222022No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCTATGC7960.10736777644841963No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTGGGCCCCAGATATCAAAAA7950.10723289230715276No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGGAC7930.10696312402461905No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTACCAGC7760.10467009362308244No Hit
CTTACCTGAGGAGACGGTGACCCAGGCCAGCCCACCACGTTTGGGACTCT7740.10440032534054873No Hit
CTTACCTGAGGAGACGGTGACCGCTGTGGATTGGGGTCATGGCCGCCGGG7730.10426544119928188No Hit
CTTACCTGAGGAGACGGTGACCAGAGATCCCTGGCCCCAGTAGTCATTGG7460.10062356938507669No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)