FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001132325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125256
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC2119116.918151625471033No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCCGAGCCCCTCAT96157.676278980647634No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC82426.58012390624002No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC62084.956249600817526No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT60034.79258478635754No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG57404.582614804879607No Hit
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG25322.021460049817973No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCAGGCTG21841.7436290477102891No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG11360.9069425815928978No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT8720.6961742351663792No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAT7390.5899916970045347No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC7270.5804113176215111No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA7070.5644440186498052No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT6830.545283259883758No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG5900.4710353196653254No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATCCC5870.4686402248195695No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG5710.4558663856422048No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT5360.42792361244171934No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCCTGGCCCCAGATATCAAAAG5050.4031742990355751No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGGC5000.39918247429264864No Hit
CTTACCTGAGGAGACGGTGACCACGGTTCCCTGGCCCCAGGGGTCGAACC4900.3911988248067957No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG4870.3888037299610398No Hit
CTTACCTGAGGAGACGGTGACCCAAATCCAGAGTCTGTAGCCCCACCCCA4870.3888037299610398No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC4690.3744331608865044No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAAG4400.3512805773775308No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCTATCACCAT4380.34968384748036024No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGAGGTCCATAG4360.3480871175831896No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATTG4340.34649038768601903No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCATTTT4280.34170019799450724No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG4250.3393051031487514No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAGAC4140.3305230887143131No Hit
CTTACCTGAGGAGACGGTGACCGAGGTCCCTTGGCCCCAGACGTCCATCC4080.32573289902280134No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGCCCATAA4000.3193459794341189No Hit
CTTACCTGAGGAGACGGTGACCACTGTCCCCTGGCCCCAGACATCAAAAC3970.316950884588363No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAGT3900.31136232994826596No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGGG3750.29938685571948653No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT3620.28900811138787763No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACCGT3550.2834195567477805No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAA3440.2746375423133423No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGAGAA3440.2746375423133423No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGCCC3420.2730408124161717No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCCCAAAGCC3420.2730408124161717No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGA3410.2722424474675864No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGAGC3220.2570735134444657No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAAAGAGTGC3180.2538800536501245No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCTG3180.2538800536501245No Hit
CTTACCTGAGGAGACGGTGACCAGGATTCCCTGGCCCCAGAGGTCAAATC3180.2538800536501245No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCGGGTC2950.2355176598326627No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAACCGACTT2930.23392092993549213No Hit
CTTACCTGAGGAGACGGTGACCAGGCTAGGGCCCTTGGTACGGGCTGAGG2880.22992910519256562No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAGGCCGAACC2870.2291307402439803No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTAATGGAAGT2830.22593728044963912No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCGTACTCCAAAAATCGTAAA2830.22593728044963912No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT2810.22434055055246854No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTTTTACA2800.22354218560388323No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGAGATCAAAAG2750.21955036086095678No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACTCAAGAC2700.2155585361180303No Hit
CTTACCTGAGGAGACGGTGACCAAGGTTCCCTGGCCCCAGTAGTCAAACT2580.20597815673500672No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCCCAC2530.20198633199208024No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGG2480.19799450724915374No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCTA2480.19799450724915374No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA2460.19639777735198316No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAATG2460.19639777735198316No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGG2440.19480104745481255No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACAT2390.19080922271188605No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCGAACC2280.1820272082774478No Hit
CTTACCTGAGGAGACGGTGACCAAAGTTCCCTGGCCCCAGAATTCAAAAG2220.17723701858593602No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTTAAAGG2150.1716484639458389No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGAGT2150.1716484639458389No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAGGG2130.17005173404866833No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAG2130.17005173404866833No Hit
CTTACCTGAGGAGACGGTGACCCCATCATAGCCCTGCTGACCCTGTGACC2120.16925336910008304No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGAGAAAGT2110.16845500415149775No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATAC2080.16605990930574183No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATATGCGTCTC1950.15568116497413298No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACTG1930.15408443507696237No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCCC1920.15328607012837708No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCATGA1910.1524877051797918No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGGGGCTCTGGTGCCGGGCTGAG1900.15168934023120648No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCATAAT1900.15168934023120648No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC1890.1508909752826212No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTTGAGGG1880.1500926103340359No Hit
CTTACCTGAGGAGACGGTGACCACCAGACCACAGGCTCAGAAACGAGGTT1880.1500926103340359No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCAAGCC1870.1492942453854506No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCCGAGCTCCTCAT1820.1453024206425241No Hit
CTTACCTGAGGAGACGGTGACCCAGACTCAGGGCTCAGGGTGACTGTCCC1810.14450405569393882No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCCAGGGCACAGTA1800.14370569074535353No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTTCCCCC1770.14131059589959763No Hit
CTTACCTGAGGAGACGGTGACCCTGAGCATGATGCCAGAGTCTTATCTGG1670.13332694641374465No Hit
CTTACCTGAGGAGACGGTGACCGCCGCGGTGGAGGAGCTTTTGGTGGCCG1670.13332694641374465No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA1640.13093185156798876No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCAAAAT1630.13013348661940347No Hit
CTTACCTGAGGAGACGGTGACCAGTGTCCCTAGGCCCCAGATATCAAAAG1620.12933512167081818No Hit
CTTACCTGAGGAGACGGTGACCCTGTGGGCTAAGGTCTCCCTTTGGCCAG1610.12853675672223286No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTATCGAAACC1580.12614166187647696No Hit
CTTACCTGAGGAGACGGTGACCCTGCCTAGGATGTCCTATCTTAAGGTGA1570.12534329692789167No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCG1550.1237465670307211No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGGGGGTTG1540.12294820208213579No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGACCCCAGTGGTCAAAGT1460.1165612824934534No Hit
CTTACCTGAGGAGACGGTGACCCAGGCCAGCCCACCACGTTTGGGACTCT1460.1165612824934534No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGC1460.1165612824934534No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCAAACC1420.11336782269911222No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCCCCCCCGCTGGT1410.11256945775052693No Hit
CTTACCTGAGGAGACGGTGACCTCCTGGGCCTAGGCCGCCTCCTTCATCC1400.11177109280194161No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGTGCTCCCGG1400.11177109280194161No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGGAGTAGC1340.10698090311042983No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTAAGAGT1330.10618253816184453No Hit
CTTACCTGAGGAGACGGTGACCTGGGGACAGGGACAGGTGGTCCTGGGAT1320.10538417321325924No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACTC1300.10378744331608865No Hit
CTTACCTGAGGAGACGGTGACCCCAGGCTGTCCTGCAGGACCAGGGACAA1280.10219071341891804No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)