FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390264
Sequences flagged as poor quality0
Sequence length300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT22503857.66301785458049No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC132423.3930877559805674No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG83822.1477768894901916No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC67771.7365168193838019No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC52781.3524178504807003No Hit
CTTACCTGAGGAGACGGTGACCAGCGTTCCCTGGCCCCAGTAGTCAAAGT42711.094387389049464No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT34380.8809421314802288No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGC27580.706701104893098No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCAGGCTG20000.5124736076092081No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC14160.36283131418731934No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTTGCCCCAGACGTCCATGT13990.3584752885226411No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCAATGT13810.3538630260541582No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTTGCCCCAGACGTCCATGT12340.3161962158948814No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT11420.2926224299448578No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG10970.28109177377365063No Hit
CTTACCTGAGGAGACGGTGACCGTGGTTCCTTTGCCCCAGACGTCCATGT9800.25111206772851197No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT8900.22805075538609765No Hit
CTTACCTGAGGAGACGGTGACCATGGTCCCTTTGCCCCAGACGTCCATGT8340.2137014943730398No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCCTGT8330.21344525756923516No Hit
CTTACCTGAGGAGACGGTGACCCAAATCCAGAGTCTGTAGCCCCACCCCA7770.19909599655617732No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG7590.19448373408769448No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAT5500.14093024209253222No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGT5250.13452432199741712No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG5180.1327306643707849No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG5150.13196195395937108No Hit
CTTACCTGAGGAGACGGTGACCAGCGTTCCCTGGCCCCAGTAGTCAAAGG5100.13068076994034808No Hit
CTTACCTGAGGAGACGGTGACCAGCGTTCCCTGGCCCCAGTAGTCAAAAG4580.11735645614250866No Hit
CTTACCTGAGGAGACGGTGACCAGCGTTCCCTGGCCCCAGTAGTCGAAGT4450.11402537769304882No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGGCGTCCATGT4310.11043806243978437No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGCCCATGT4270.10941311522456594No Hit
CTTACCTGAGGAGACGGTGACCGTGGTTCCCTTGCCCCAGACGTCCATGT4180.1071069839903245No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGA4130.10582579997130148No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGG4060.10403214234466925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)