FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001132331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334637
Sequences flagged as poor quality0
Sequence length300
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC28559985.34591213762972No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATAC25240.7542501277503684No Hit
CTTACCTGAGGAGACGGTGACCATGGTCCCTTGGCCCCAGACGTCCATAC15650.46767093895773626No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC12940.3866876645439685No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCAATAC11800.3526208996614241No Hit
CTTACCTGAGGAGACGGTGACCGTGGTTCCTTGGCCCCAGACGTCCATAC11480.3430582989926398No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC10690.31945062859157836No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATCC10370.30988802792279396No Hit
CTTACCTGAGGAGACGGTGACCGTGGTTCCCTGGCCCCAGACGTCCATAC9400.28090139464554126No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC9130.2728329503312545No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT7470.22322695936193548No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGC6520.19483798862648183No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCCTAC6100.18228707524870233No Hit
CTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACC4610.13776121588467502No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC4310.12879627775768968No Hit
CTTACCTGAGGAGACGGTGACCGTGGCCCCTTGGCCCCAGACGTCCATAC4210.12580796504869457No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT4200.12550913377779505No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGAAGTCCATAC4060.12132549598520188No Hit
CTTACCTGAGGAGACGGTGCCCGTGGTCCCTTGGCCCCAGACGTCCATAC3900.11654419565080969No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGGCGTCCATAC3810.11385471421271408No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCGTAC3680.10996990769102041No Hit
CTTACCTGAGGAGACGGTGACCGTTGTCCCTTGGCCCCAGACGTCCATAC3610.10787808879472384No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGCCCATAC3530.10548743862752773No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAAAC3420.10220029464763311No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)