FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001132332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179979
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT2099511.665249834702937No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT159938.886036704282166No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATTGC141167.8431372549019605No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC85224.7349968607448645No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTACTCGAAAG82074.559976441695976No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG50862.825885242167142No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG25921.440168019602287No Hit
CTTACCTGAGGAGACGGTGACCGTTGTCCAGGGGCCTGTCGCACCCAGAG19131.0629017829857927No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG13970.7762016679723746No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGTAAGCAAACC13740.763422399279916No Hit
CTTACCTGAGGAGACGGTGACCTCCTGGGCCTAGGCCGCCTCCTTCATCC10930.6072930730807483No Hit
CTTACCTGAGGAGACGGTGACCGTGGTTGTCCAGGGGCCTGTCGCACCCA10740.5967362859000217No Hit
CTTACCTGAGGAGACGGTGACCCCTGTCCTTCTCACGGGGGGTCTCCAGC8890.49394651598242023No Hit
CTTACCTGAGGAGACGGTGACCCGGCCAGGGCTGGGGGCTGGGGGTGAGT8300.4611649136843743No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC7900.438940098567055No Hit
CTTACCTGAGGAGACGGTGACCCAGGCCAGCCCACCACGTTTGGGACTCT7580.42116024647319966No Hit
CTTACCTGAGGAGACGGTGACCCTGTGGGCTAAGGTCTCCCTTTGGCCAG6660.37004317170336537No Hit
CTTACCTGAGGAGACGGTGACCTCTGTGGCTGCTGCACGGCACAGCCGAG5840.32448230071286094No Hit
CTTACCTGAGGAGACGGTGACCTGGGGACAGGGACAGGTGGTCCTGGGAT5700.3167036154217992No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCACGGCCCCAGAGATCGAAGT5380.2989237633279438No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT5160.28670011501341824No Hit
CTTACCTGAGGAGACGGTGACCATGCAGACACCACACGCTCTCCTGCAGT5000.2778101889664905No Hit
CTTACCTGAGGAGACGGTGACCAAGCCATCAGGGACCGGTTCTCCCGGCT4660.25891909611676917No Hit
CTTACCTGAGGAGACGGTGACCCTGTCTGTAAAAAAAAGTTCTTAATTTT4340.24113924402291378No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGGGGCTCTGGTGCCGGGCTGAG4160.23113807722012014No Hit
CTTACCTGAGGAGACGGTGACCGTGGTTCCTGCGCCCTCTCGATGGGGAC4030.22391501230699137No Hit
CTTACCTGAGGAGACGGTGACCCTGCCTAGGATGTCCTATCTTAAGGTGA4020.2233593919290584No Hit
CTTACCTGAGGAGACGGTGACCTCGCCGTCCACCTCGGCGACGAGCCAGG4020.2233593919290584No Hit
CTTACCTGAGGAGACGGTGACCATGCCACTGGACACCTCTGGTGCTGACT4020.2233593919290584No Hit
CTTACCTGAGGAGACGGTGACCTGGCAAGAAAGAGAAATGATGGGCAATA3920.21780318814972857No Hit
CTTACCTGAGGAGACGGTGACCCCCTCCCCTTCCTCAGACTTATATAGGA3800.2111357436145328No Hit
CTTACCTGAGGAGACGGTGACCCCAACATGCGGCCTTTACCCTGGGGAGG3750.2083576417248679No Hit
CTTACCTGAGGAGACGGTGACCCAAATCCAGAGTCTGTAGCCCCACCCCA3720.20669078059106896No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCTTTGTCCAGGGGCCTGTCGCA3720.20669078059106896No Hit
CTTACCTGAGGAGACGGTGACCATGGGAGGGGGCTGCAGAGAGTCTCAGA3690.20502391945727No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGTCCAGGGGCCTGTCGCA3580.19891209530000722No Hit
CTTACCTGAGGAGACGGTGACCAGTGATGGCTCCCGCAGGAGCAGCGGGG3220.1789097616944199No Hit
CTTACCTGAGGAGACGGTGACCAGCACCCTCATGCTGACATCAGATGTCA3130.17390917829302308No Hit
CTTACCTGAGGAGACGGTGACCCCAGCCCCTCCCTTCCTGGCTTCAGTAC3080.17113107640335817No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACTCGAAAG2950.1639080114902294No Hit
CTTACCTGAGGAGACGGTGACCATCCCTGAGTGTGTGCACATTCCACCCT2920.16224115035643047No Hit
CTTACCTGAGGAGACGGTGACCTCTCTCCAGGCTCTAAGGGAGAGCGGGT2910.1616855299784975No Hit
CTTACCTGAGGAGACGGTGACCTTCCCTGGGGGCTGGCGGATCCAAGATC2870.15946304846676557No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCCGGCCCCAGAGATCGAAGT2860.15890742808883257No Hit
CTTACCTGAGGAGACGGTGACCGTGGAAGTTAAGTTTGTCCAGGGGCCTG2850.1583518077108996No Hit
CTTACCTGAGGAGACGGTGACCCCAGCCCCTCAACTGGAGATGTGGACAG2780.15446246506536876No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC2760.15335122430950276No Hit
CTTACCTGAGGAGACGGTGACCTGGCCTTGGAAGGACGGGCTGTATCTGG2760.15335122430950276No Hit
CTTACCTGAGGAGACGGTGACCCAGTTGGCCAGTAGCCCACCCAGATCTT2640.14668377977430702No Hit
CTTACCTGAGGAGACGGTGACCTTCCCTGGGGGCTGACGGATCCAAGATC2490.1383494741053123No Hit
CTTACCTGAGGAGACGGTGACCTCAGAGCCCCAGACTCGGCCCAGTGAAC2460.13668261297151335No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCATGGCCCCAGAGATCGAAGT2450.13612699259358035No Hit
CTTACCTGAGGAGACGGTGACCCACAGTGTGACACTTTGCAGGGACCAGG2430.1350157518377144No Hit
CTTACCTGAGGAGACGGTGACCCGGCAGCAGCACATGGCACCCGCGCAGA2330.12945954805838458No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGTCCAGGGGCCTGTCGC2300.12779268692458565No Hit
CTTACCTGAGGAGACGGTGACCCCAGGCTGTCCTGCAGGACCAGGGACAA2290.12723706654665265No Hit
CTTACCTGAGGAGACGGTGACCACACGGCGCAGCTGCTTTGCACCCGGGA2270.1261258257907867No Hit
CTTACCTGAGGAGACGGTGACCCAGCCTGGAGTCGCCCATTCCGCCCCCA2240.12445896465698776No Hit
CTTACCTGAGGAGACGGTGACCCCGGGTGGTGAGACCTTAGGCTGTGAGC2140.11890276087765796No Hit
CTTACCTGAGGAGACGGTGACCCTGTCCTCATGTCCTGGCACCCGTGTTG2140.11890276087765796No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGC2110.117235899743859No Hit
CTTACCTGAGGAGACGGTGACCTTTCAGGTTCTTCCCACCTCCGAGCTTT2100.11668027936592602No Hit
CTTACCTGAGGAGACGGTGACCCTCTCCAGTGAGGGTGGGTCAGAGTAGA2080.11556903861006007No Hit
CTTACCTGAGGAGACGGTGACCAGGCTAGGGCCCTTGGTACGGGCTGAGG2070.11501341823212709No Hit
CTTACCTGAGGAGACGGTGACCCATTCACAGGCTCCAGAGATTAGGAGAT2040.11334655709832814No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGGTCGAAGT1990.11056845520866322No Hit
CTTACCTGAGGAGACGGTGACCTGAAACTGCGTGGATCTGCTGTTTCCAT1960.10890159407486429No Hit
CTTACCTGAGGAGACGGTGACCAGCCCAGGCAATGTAGCTGGTAAACGGC1940.10779035331899833No Hit
CTTACCTGAGGAGACGGTGACCAGCGCAGACAGTTGGGACTTCACAGGGA1930.10723473294106535No Hit
CTTACCTGAGGAGACGGTGACCTTAGGTGATGAGTTATTTAGGCTCTTTG1930.10723473294106535No Hit
CTTACCTGAGGAGACGGTGACCTGAGAGGTGGAGTACAGGACTGGACCTT1900.1055678718072664No Hit
CTTACCTGAGGAGACGGTGACCGTTGTCCAGTGGCCTGTCGCACCCAGAG1840.10223414953966853No Hit
CTTACCTGAGGAGACGGTGACCTCCTTCCCTGGGGGCTGGCGGATCCAAG1830.10167852916173553No Hit
CTTACCTGAGGAGACGGTGACCACGGAAGGAACCCCACCCTCTGTCCCCA1820.10112290878380255No Hit
CTTACCTGAGGAGACGGTGACCGACAGCGCTGGGTGAAATCCCGCACCGT1820.10112290878380255No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)