FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001132340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8588
Sequences flagged as poor quality0
Sequence length35-300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAT681179.30833721471822No Hit
CTTACCTGAGGAGACGGTGACCCTGCTGCTGGGCCTGCAGGATGTCCACA1001.1644154634373545No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCCTGGCCCCAGATATCAAAAT840.9781089892873778No Hit
CTTACCTGAGGAGACGGTGACCATGGTCCCTTGGCCCCAGATATCAAAAT690.8034466697717745No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC540.6287843502561714No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATGTCAAAAT490.5705635770843037No Hit
CTTACCTGAGGAGACGGTGACCTTGGGGGAAAGCAAAAAGGGGCAGAGAT450.5239869585468095No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT420.4890544946436889No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC390.45412203074056817No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG380.4424778761061947No Hit
CTTACCTGAGGAGACGGTGACCCCTGTGCTCCCCTGTGGTGGGTGTGGGG300.3493246390312063No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT300.3493246390312063No Hit
CTTACCTGAGGAGACGGTGACCATGGTCCCTTGGCCCCAGATGTCAAAAT290.3376804843968328No Hit
CTTACCTGAGGAGACGGTGACCATTGTTCCCTGGCCCCAGATATCAAAAT250.2911038658593386No Hit
CTTACCTGAGGAGACGGTGACCTCTCTGCGTGGGAGGGGCCGGTGTGAGC230.26781555659059153No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAACT190.22123893805309736No Hit
CTTACCTGAGGAGACGGTGACCAGTGTCCCTTGGCCCCAGATATCAAAAT160.18630647414997673No Hit
CTTACCTGAGGAGACGGTGACCATTGTTCCTTGGCCCCAGATATCAAAAT160.18630647414997673No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAC160.18630647414997673No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCCTGGCCCCAGATATCAAACT150.17466231951560315No Hit
CTTACCTGAGGAGACGGTGCCCATTGTCCCTTGGCCCCAGATATCAAAAT150.17466231951560315No Hit
CTTACCTGAGGAGACGGTGACCCTTGTCCCTTGGCCCCAGATATCAAAAT140.16301816488122964No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTCGGCCCCAGATATCAAAAT140.16301816488122964No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAATAT140.16301816488122964No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTTGCCCCAGATATCAAAAT120.13972985561248255No Hit
CTTACCTGAGGAGACGGTGACCATTTTCCCTTGGCCCCAGATATCAAAAT120.13972985561248255No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAGT110.12808570097810898No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGACATCAAAAT110.12808570097810898No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCCAAAT110.12808570097810898No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATCTCAAAAT90.10479739170936189No Hit
CTTACCTTAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAT90.10479739170936189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)