FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541860
Sequences flagged as poor quality0
Sequence length35-300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT24493745.20300446609826No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC21645239.94611154172665No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGC45930.8476359207175285No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTTGCCCCAGACGTCCATGT34170.6306056915070314No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCAATGT13720.2532019340789134No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTTGCCCCAGACGTCCATGT10570.19506883696895877No Hit
CTTACCTGAGGAGACGGTGACCCGGGTTCCCTGGCCCCAGGGGTCGAACC10060.18565681172258516No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTTGCCCCAGGGGTCGAACC9190.16960100394935962No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCGAACC9040.16683276122983795No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC8610.15889713210054257No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC8300.1531760971468645No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAAC8210.15151515151515152No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAGC7890.14560956704683867No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGT7380.13619754180046506No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGCGTCGAACC7120.13139925441996086No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCGAACC6810.1256782194662828No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGC6800.125493669951648No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAGGTCGAACC5650.10427047576864873No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)