FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583065
Sequences flagged as poor quality0
Sequence length43-300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC12893722.113657996964317No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT412197.069366194163601No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC376116.450567260940032No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG341535.857494447445825No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT181323.1097733528851843No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC89581.536363870237452No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC80581.3820071518612846No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA74161.2718993594196186No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT70921.2163309408041985No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG48700.8352413538799276No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC40150.6886024714225687No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT33680.5776371416565905No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG32080.5501959472786053No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG31920.5474518278408068No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA26200.44934955793950926No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT24350.4176206769399638No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG23080.395839228902438No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAAAC19160.32860830267637403No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC18690.3205474518278408No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG18100.31042851140095873No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG17990.30854192928747226No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGGT17420.298766003790315No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAT16390.28110073490948695No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGT16070.2756124960338899No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC14990.2570896898287498No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAT14940.25623215250443776No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACT14210.24371210756948197No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT14070.24131100306140824No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGC13480.23119206263452616No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT12940.22193065953195612No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT12620.21644242065635907No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACGG12340.21164021164021166No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT12100.20752403248351384No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCACAC11860.20340785332681605No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC11450.19637604726745733No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATAC11270.19328891289993397No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAC11140.19105931585672264No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATTC10780.18488504712167597No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGA10610.18196942021901505No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAGTCAAAGT10580.1814548978244278No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATTG10530.18059736050011577No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAA10220.1752806290893811No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG9990.17133595739754573No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCC9950.17064992753809607No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACAA9490.16276058415442532No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGGAT9220.15812988260314031No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAGC9210.1579583751382779No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCC9020.15469973330589215No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAG8960.1536706885167177No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAAGG8740.14989752428974473No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATTGG8680.14886847950057028No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCAGGCTG8610.1476679272465334No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGTC8610.1476679272465334No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATTC8600.147496419781671No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGT8470.1452668227384597No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCAA8360.1433802406249732No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG8330.142865718230386No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC8020.13754898681965133No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGCCCATAC7770.13326130019809113No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACA7490.1284590911819437No Hit
CTTACCTGAGGAGACGGTGACCAGGCTTCCCTGGCCCCAGGGGTCGAACC7490.1284590911819437No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCT7460.12794456878735647No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACGT7460.12794456878735647No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGC7330.12571497174414517No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGGAGTCAAAGT7210.12365688216579628No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGTACC6940.11902618061451124No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAGAC6860.11765412089561199No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATTTG6800.11662507610643753No Hit
CTTACCTGAGGAGACGGTGACCACGGTTCCCTGGCCCCACGAGTCGAACC6570.11268040441460214No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAAGT6530.11199437455515253No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCGTGGCCCCAGGAATCAAAGT6530.11199437455515253No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTCGGCCCCAGATATCAAAAG6510.1116513596254277No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCT6440.11045080737139083No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTAGGCCCCAGATATCAAGAG6340.10873573272276676No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACTCC6310.10822121032817954No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTAATAAG6300.10804970286331712No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATTTA6280.10770668793359231No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGAGT6280.10770668793359231No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGGCGTCCATAC6260.1073636730038675No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCTTCAC6110.10479106103093137No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGG6090.10444804610120655No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGGCACCAA6090.10444804610120655No Hit
CTTACCTGAGGAGACGGTGACCAGAGTTCCCTGGCCCCAGTAGTCAAAGC6050.10376201624175692No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGG6010.1030759863823073No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGTTC5990.10273297145258248No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGTAGTCTACCT5930.10170392666340802No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTACAC5910.10136091173368321No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGGCT5860.10050337440937115No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAAGAGTCAAAGT5860.10050337440937115No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGCCA5850.10033186694450877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)