FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417988
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC27710.6629376919911577No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGGACGAGCTGCTATT10620.2540742796443917No Hit
CCATCATAGCCCTGCTGACCCTGTGACCTTGTGGCAGCCCCTCTGGCCCC9770.23373876762012308No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA9220.2205804951338316No Hit
CACACCAAGCTCAGAGAGGGCAGGGGTGGGAAGGAGACATCAGATGTGGG6570.15718154588169994No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAGAGGTAATA5810.13899920571882446No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAAACAACCACTCC5750.13756375781122904No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCTCCCACT5740.13732451649329647No Hit
ATTGTCCCCTGGCCCCAGATATCAAAAGCATCATGCCTCGTCAGCCACTG5690.13612830990363362No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTAAGGACA5610.13421437936017303No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTATGAGG5540.132539690134645No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATGATAGCTCCCACTATACTC5540.132539690134645No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATGGCGGTCC5430.12990803563738673No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGACAGCTGC5420.12966879431945416No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGAAAGTTCCGGA5410.12942955300152156No Hit
GTGGTCCCTTGGCCCCAGACGTCCGAACCGTAATAGCAATTGCTGCTACA5380.12871182904772385No Hit
ATTGTCCCTTGGCCCCAGATTGTGACTCCCCGAACCCCCCAATCTCTCGC5380.12871182904772385No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGGAAGAGGTTGCCAG5360.1282333464118587No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGGAATCGTAGCC5320.1272763811401284No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGCTATCAGCTGCTGCTAT5210.12464472664287013No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGACCCGGGGTAAAATCG5170.12368776137113985No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAAACTCCCCGAACCAAAGT5100.12201307214561184No Hit
AGGGTTCCCTGGCCCCAGGGGTCGACAGTAGCTCCCACGACTGTTGCACA5100.12201307214561184No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAACCCCC5090.12177383082767927No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGAGAGAATCGTAATATAA5080.12153458950974669No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGGTGGTAGCTCCCACCAGGCTCTCGC5060.12105610687388153No Hit
ATTGTCCCCTGGCCCCAGATATCAAAAGAGTCATCGTAATAACCACTACT4980.11914217633042098No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTACGATAG4980.11914217633042098No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAACGGCC4980.11914217633042098No Hit
AGGGATCCCTGGCCCCAGTAGTCAAGGCACCCGCTATGGGGGGGCTCTCT4930.1179459697407581No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTTGGCAA4930.1179459697407581No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAAACTCTACCTTT4910.11746748710489296No Hit
ATTGTCCCTTGGCCCCACATATCAAAAGCATCATGGTAGCTCCCCCATTT4890.11698900446902782No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTTCTTCACTTCTGCTGCTA4880.11674976315109524No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCCTTTTCCAAAAATCGTAAT4860.1162712805152301No Hit
GCGGTCCCTTGGCCCCAGACGTCCAGACCGTAGTTGTAGAGGTAAATGAG4850.11603203919729754No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTATTCCGACAAAGTAGCTCCCAC4850.11603203919729754No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATTGGCGCTC4850.11603203919729754No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTAGTAATAACCACTACT4850.11603203919729754No Hit
ATTGTCCCCTGGCCCCAGATATCACAAAGGATGTGGCAGCTGGTAACATT4840.11579279787936496No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATCCAAAACG4810.11507507392556723No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGCCCTTGAAACC4800.11483583260763466No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCGTGGCCATAGCTGTATCC4790.1145965912897021No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGATTCCCTTTTGTAGCCT4790.1145965912897021No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTCCCCCGTATACTGCAAATTTCT4770.11411810865383695No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGTTCCGG4760.11387886733590438No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGCCCCGAC4740.11340038470003923No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAATAACCACT4710.11268266074624153No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCGAAGCTGCTACCGCCTCT4700.11244341942830895No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAGGGGCGGG4690.11220417811037638No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGGCGTAACCACTCCAAA4680.1119649367924438No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAAAATTGT4680.1119649367924438No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATTGGTGGGGTAACCACTACT4680.1119649367924438No Hit
GTGGTCCCCTGGCCCCAGACGTCCATATCGTAAGCGTAGTAGGGGGGAAT4660.11148645415657865No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGACATTATAATA4660.11148645415657865No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCACCCCC4650.1112472128386461No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCGTTCCC4650.1112472128386461No Hit
AGGGTTCCCTGGCCCCAGTAGTCATTCGTTTTGTTACCACCGGGGAAACT4620.11052948888484837No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTATCCGAT4620.11052948888484837No Hit
AGGGTTCCCTGACCCCACCTGTCAAAGTCACCCCAGCCATCGCTATTCGC4610.1102902475669158No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTGGCCTATTATAAT4610.1102902475669158No Hit
AGGGTTCCCTGGCCCCAGAGGTTTATAGCTCTCCAGCCGTCTCTCGCACA4610.1102902475669158No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCACGGGCCCACCTCCTCGC4600.11005100624898322No Hit
GTGGTCCCTTGGCCCCAGACGTCCAAACCGTTACTGAGGAGAAGCTCCCA4600.11005100624898322No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTGTTTGGT4600.11005100624898322No Hit
AGGGTTCCCTGGCCCCACTAATGAAAGGCGCGTTAGCACTTATTAGCATT4600.11005100624898322No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGGTCCCGCGAATAACCACTCCAA4600.11005100624898322No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCGTCTACCACCGTACTTGA4590.10981176493105065No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCCGTACTC4590.10981176493105065No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTGGGCGTA4570.10933328229518552No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTCCAGAGGGGCATC4560.10909404097725293No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTTAATAACCACTACTATCAT4540.10861555834138778No Hit
CGGGTTCCCTGGCCCCAGTAGTCAGCCCCCTCCGAGTAATAACCACCCAA4510.10789783438759008No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATTGCC4490.10741935175172493No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCTCCATA4470.10694086911585977No Hit
AGGGTTCCCTGGCCCCAGTGGTCAAAGTAGAAAAGTCCCCGAACCCCAAG4460.1067016277979272No Hit
AGGGTTCCCTGGCCCCAGTAGTCTTCAGCAGCTGGTACTACTACAAAAGC4460.1067016277979272No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGGACGAGCTGCTCCGTCGTCTCT4450.10646238647999465No Hit
ATTGTCCCAGGGCCCCACACATCAAAAGCAAACCACCAATTTTTCCTATA4440.10622314516206208No Hit
AGGGTTCCCTGTCCCCAGTAGTCAAAGTGGGGGGTGGGGTCGTTCCAGTT4430.10598390384412949No Hit
AGGGTTCCCTGGCCCCAGGAATCAAAATAATAGAGGAGTATAGCAGTTGG4410.10550542120826435No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGATGGTA4400.10526617989033177No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAGGA4400.10526617989033177No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTCGCTGCC4400.10526617989033177No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGCGCTTGGGGT4400.10526617989033177No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGCGTTTCCTTGTACCAGC4390.1050269385723992No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAATAACCAGT4390.1050269385723992No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTGGAACCCGTAGCAGCT4380.10478769725446664No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCGTAATAACC4370.10454845593653407No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATAGGTC4360.10430921461860149No Hit
AGGGTTCCCTGTCCCCAGTAGTCATCAATGTAAGAATAACCATAGCTGCT4360.10430921461860149No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTACTGGTAGCT4350.10406997330066892No Hit
ACGGTTCCCGGGCCCCAGTAGTCAAAGGCCTCTGGGTTCCAGTTATCCGA4350.10406997330066892No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTACGACTCAACCCAATAATTCCC4350.10406997330066892No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTGGCAGCTGGTACTACT4340.10383073198273635No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGCGCCC4340.10383073198273635No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTCTGTACCAG4340.10383073198273635No Hit
AGGATGCCCTGGCCCCAGTGGTCAAAAGCGGCAACTCCCCCAAATGTAAG4320.1033522493468712No Hit
AGGATTCCCTGGCCCCAGTAGCCGAGAAGAAAGTCACCGTCGTCATAAGA4320.1033522493468712No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTTGGCCCCGTCTGGGTC4310.10311300802893864No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTCCCCACCTACCCACTCCAA4300.10287376671100606No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATGGGG4290.10263452539307348No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTTGGTGC4290.10263452539307348No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTGCTTTGTCAA4290.10263452539307348No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGAGAGGAGTAATAACCACTAC4270.10215604275720834No Hit
AGGGTTCCCTGGCCCCAGTAGTCCCCGGGGTCACCGTAGTAGCCCCCCGC4270.10215604275720834No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGCCACCGTGTACCCCACTACT4260.10191680143927576No Hit
AGGGTTCCCTCGCCCCAGAGCTTTTTAAGCACTGAAATCCTGTACCAGCT4250.1016775601213432No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCAAGGGCC4250.1016775601213432No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATATATTGGGCTACTATC4250.1016775601213432No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAACCCGG4240.10143831880341063No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTCCATTTTCCCCCTTCG4210.1007205948496129No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGGTCAGC4200.10048135353168033No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGGTCGCTATATCAAGATCTCT4200.10048135353168033No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACCCTCTATCATAAGG4190.10024211221374778No Hit
GGGGCACCTTTGTCCCAGACGTCCATGGAGTAGTAATTGGAGTAAGGGGT4190.10024211221374778No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGGAGCATCTCTCGCACAGTAATA4180.1000028708958152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)