FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367447
Sequences flagged as poor quality0
Sequence length300
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGGAGATAGCAGCTGGTACTAC131023.565684302770195No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTATTCATTTTTCGGCGGGACCAG39051.06273829967315No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTGCGCCTGATCCTACTAG13290.36168481440860856No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATAGTCTACTATCATAGTAAT5560.15131433921082496No Hit
ATTGTCCCTTGGCCCCACACATCAAAAGCACCAAAGCCAACAAAATCTTT5550.15104219111871917No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCTAAACCATAGCTGTGTCC5420.14750426592134375No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATGCCCCGAGGGGCAGCTACC5300.14423848881607415No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACCCCAGGTTATT5290.14396634072396836No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACCGACCAAGGGG5260.14314989644765094No Hit
ATGCTTCCCTGGCCCCAGTAGTCAATGTAGTCGTAGCCATCGTGGAATCT5260.14314989644765094No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACCCATCCCAGCCACTGGAAT5180.14097271171080455No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATTGTT5140.13988411934238135No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTTTTTATTCCCCGAACC5100.13879552697395817No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGCCGTAGTAATAACCACT4950.13471330559237116No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTGGGTAAC4900.13335256513184213No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCCCCCTCCGGAGTAGCAGCTACC4890.13308041703973633No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGAAAGACTCACC4880.13280826894763054No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGCGAGCAG4870.13253612085552474No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGACCCTATAACTCCCCGAACC4840.13171967657920736No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTCCGAATAATAACC4840.13171967657920736No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCACCCGCCCATAACCACT4740.12899819565814935No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGCGAGTAAACTGATC4690.12763745519762032No Hit
ACAGGTCTGCCGCCTTGCAATGCGACGCCTGCCTGGGAGCGCAGGTGAGG4670.12709315901340873No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACCACCTCTTCACCCG4670.12709315901340873No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGAACCGCGTACCAGCTGCTGC4650.12654886282919714No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTACTCTGG4640.12627671473709134No Hit
TCACGGGGAACCCTGGATGAGAAGCTACGGTGTAAGTCCTGCCCCCTTGG4610.12546027046077393No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAAAAGTCACCGTAGTGCCCGAC4600.12518812236866814No Hit
AGGGTTCCCTGGCTCCAGTAGTCAAAGTTTCCGGTTGCTGGTGCAACTCT4590.12491597427656234No Hit
AGGGTTCCCCGGCCCCAGTGGTCAAAGTACTCACACGATGTTCTTGGATT4560.12409953000024493No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATTAGG4550.12382738190813913No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGATGGGG4530.12328308572392753No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTCTGCGCCATAATAACCACT4510.12273878953971594No Hit
CCAGTGCAGGCCAACCCCACCTCCCTCCTCACGAGCAGGTGTGAAGAACG4480.12192234526339854No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTGTGAGGGACGAGCTGCTAGGCC4460.12137804907918694No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAACTGGTTGTAGCCATCGTCTTCGCC4460.12137804907918694No Hit
AGGGTTCCCTGGCCCCAGTAGTCACAAAGGGACCATAGCAGCTGGTACTG4410.12001730861865792No Hit
GTGGTCCCGTGGCCCCAGACGTCCATACCGTAGTAGTAATAACTCCCACT4370.11892871625023474No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTACCCCCCGTCGTTCCAGTCAAA4360.11865656815812893No Hit
AGGGTTCCCGGGCCCCAGGAGGGGTGAGGGTCCCTCGCACAGTAATAAAC4350.11838442006602314No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAGGACGTCGTACCAGCTGC4340.11811227197391734No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCGGACGAGCTGCTATAGG4330.11784012388181152No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTTCCTCCATAGGCAGAATCTTTC4310.11729582769759991No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTATAGGGCACGGCAGCTACC4310.11729582769759991No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCCCACGGTCTTCCCGAACC4290.11675153151338832No Hit
AGGGTTCCCTGGCCCCAGTAGTCAACACCTATCATAGGTTTTCGATCTCT4260.11593508723707092No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAGTCCCCGTTACCACCGT4260.11593508723707092No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGATCGGCGTACCCCCCGTATCTCT4240.11539079105285933No Hit
AGGGTTCCCTGGCCCCAGGAGTCAAAGTAGTAAAAGCTGCCGGCACGGGC4230.11511864296075353No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGGTGCTCGG4230.11511864296075353No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGCCAATCGTAGCCACTATTATTCC4210.11457434677654191No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGATTATTAT4210.11457434677654191No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCGGGGGGAGGATCTCGCACAGTA4190.11403005059233032No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTTGAAAAAGTCGTGTTCTTT4190.11403005059233032No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGGGGGTTAATAA4190.11403005059233032No Hit
CACCAGGCCCGGGCGACCACGGTGCATGCCGTTCGGGACTCAGAATTGGC4190.11403005059233032No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCAAACGGGAT4180.11375790250022452No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTGCCTCCCGTGGGCGAACCA4160.11321360631601292No Hit
GTGGTCCCTTTGCCCCAGACGTCCATCCGACGAGCTGCTATATCTCTCGC4140.11266931013180133No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCATACTCATAGTCCCCGCGAGT4130.11239716203969552No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAACGAAACCCAGGTTCCAGTTGGGGA4130.11239716203969552No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATTCCCCCTAAAACCAGT4120.11212501394758972No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAAAAGACTCCCCGATCTCTCGC4120.11212501394758972No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGACGGTACCA4100.11158071776337813No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCCCTGTCGGAG4100.11158071776337813No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAAAAGTAGTAATAACCACT4100.11158071776337813No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGGCTGCGTAACCATCCCCACGCCG4090.11130856967127231No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACCCTCCAATATGCAC4080.11103642157916652No Hit
AGGGTTCCCTGGCCCCAGTATTCCATCGCAGCTGGTACTACTACACCCTC4080.11103642157916652No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGGCTCGGGTAGGTACCA4080.11103642157916652No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCATCCGAGACATCTCTCGC4070.11076427348706071No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCAAAAAGGGCCGATGTTCCAGT4070.11076427348706071No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATTCCCACCA4070.11076427348706071No Hit
GTGGTCCCTTGGCCCCAGACGTCCATATCGTGGTAGTAGGCTAACTGACC4060.11049212539495491No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATGGCCA4040.10994782921074332No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGGAGTTGTCCAGCGG4040.10994782921074332No Hit
AGGGTTCCCTGGCCCCAGTAGTCCCAGTACCAGCCACTGCTATACCCGGT4040.10994782921074332No Hit
GTGGCCCCTTGGCCCCAGACGTCCATACCGTGATAGTAGGCGTCATAGCA4040.10994782921074332No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCTAGGAT4020.10940353302653172No Hit
AGGGTTCCCTGGCCCCAGTAGTCCGATCCGGCGTAGCCACTATAATTAAC4020.10940353302653172No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGAAGTAGTGGAAGCGCCT4000.10885923684232013No Hit
AGGATTCCCTGGCCCCAGTCTTCAAAGTAAAATGTAGTCCCCGAACCACG3990.10858708875021432No Hit
GTGGTCCCTTGGCCCCAGACATCCATAGCGAAATAGTAATAGTAAGTACT3980.10831494065810852No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATGAGGGGGAACCATAATAACCACT3960.1077706444738969No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTCGAAAAGGCA3950.10749849638179111No Hit
GTGGTCCCTTGGCCCCAGACGTCCATTCTGTCGCTATGCCCGGGCTCTGT3950.10749849638179111No Hit
AGGGTTCCCTGGCCCCAGACCTGCTCCCACCCCCGATCTCTCGCACAGTA3940.1072263482896853No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCCGTAAGACGAGCTGCTATA3940.1072263482896853No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCCCAAGGTACCAGCCAACGCCC3930.10695420019757951No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGCCTAGGTCGTTGGTGCTCTC3900.10613775592126212No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCCGGGGCAGCTGGTACTAC3890.10586560782915631No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAACATAAC3890.10586560782915631No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTCCAGAGA3880.10559345973705052No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGTAGCTCCCACCTTTTATTTACTTT3860.10504916355283891No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGGAACCGAGTAGCTCCCACTT3860.10504916355283891No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCACACCATCACCGTAGTC3850.10477701546073312No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCCACTCCAAAA3850.10477701546073312No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAGCA3840.1045048673686273No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATTTGAATAGCAGCTGGTACT3830.1042327192765215No Hit
AGGGTTCCCTGGCCCCGGGGCTCCAACTCCCCAATAACCACTACTATCAT3830.1042327192765215No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCCCGAAAGGAATAACTCCCTGAA3830.1042327192765215No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGCGTAGTAGTAGGGTCTCCCCGA3820.1039605711844157No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAGGTCC3810.10368842309230991No Hit
AGTACAAATGCTGCCAAAATCTTCAATTTTTACCCAAGGAAGGGGCGAGT3810.10368842309230991No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTGTCCGGGGGAGAACCAGTC3810.10368842309230991No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGGTGCCCCTTGACCGCTGCTATACTT3780.10287197881599251No Hit
AGGGTTCCCTGGCCCCAGCGGTCGAAGTCACCGCCCGGGGCTGTCGAACA3770.10259983072388672No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTACCCCCTCTT3760.10232768263178091No Hit
GTGGTCCCTTTGCCCCAGACGTCCGAGTCGAAGTCACCGTTTAGTCTCCC3750.10205553453967511No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACGAGGTGAGACTACAAATCGCCCC3740.10178338644756932No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAACGTAGCTCCCACTCCTCTCT3740.10178338644756932No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTCTTTACG3740.10178338644756932No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGCAGTAACCTTTCAC3740.10178338644756932No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCCAACCACTCCAAAAATCGT3740.10178338644756932No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGGGGGTA3730.10151123835546352No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCGCACATCGGCCGCCAGTTACC3720.1012390902633577No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTACCCTGTAGCTCCCACTATACC3720.1012390902633577No Hit
AGGGTTCCCTGGCCCCAGGGGTCGTCGGGTTGCCTCGATCCTCTCGCACA3720.1012390902633577No Hit
GTGGTCCCTTGGCCCCAGACGTCCATTTCGTAGTTGTAGTAGTATGACCG3710.1009669421712519No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCAAGTATT3710.1009669421712519No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCCGT3690.1004226459870403No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAGTA3690.1004226459870403No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTATATGGCTGACCGCCCCTC3690.1004226459870403No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCCCCCACAAGATTGCTATAGCA3690.1004226459870403No Hit
GGCACACGGTACACGTACGTGCAGATGTGCGAGCCACAAACGCATGGAAA3690.1004226459870403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)