FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001132357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218727
Sequences flagged as poor quality0
Sequence length300
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGGAGATAGCAGCTGGTACTAC43610.3578323939651784No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAACC32190.26412806149367335No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCAGGGACCACTCCAAAAA18850.15466958555935825No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCTTT18840.15458753272882278No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCTCGACCCTCCCTGAACCATAGT18620.1527823704570425No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTGGGCCACCGTAGTCTCTCTCGC18230.14958231006615919No Hit
GTGGTCCCTTTGCCCCAGACGTCCAAGTGGTAGTAGTAGTAGGACACACA18090.14843357043866262No Hit
AGCTCTTGGCGTTGTCACTGGAGATGGTGAATCGGCCCTTCACAGAGTCC17780.1458899326920631No Hit
AGTATTCCCTGGCCCCAGGGGTCGAACCAGCGAGTAGCAGCAACCGCCAC17560.1440847704202828No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATGT17290.14186934399582515No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTACTTAGCAGCTGG17010.13957186474083202No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGGAGTAGGAGTAGTACCAGCCACT16690.13694617416369703No Hit
AGGGTTCCCTGGCCCCAGCTATCGTAGTTCCCCACGCCTAGGGCGAGCGT16650.13661796284155517No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATCATGGCCTCCCTTTAT16400.13456664207816846No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTATAGTATTGGGCCCAGCC16280.1335820081117428No Hit
AGGGTTCCCTGGCCCCATTCTTCTGAGTAATAACCACTACTATCATAGTA16210.13300763829799453No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATAAATGGTAATAACCACTACTATC16180.13276147980638814No Hit
AGGGTTCCCTGGCCCCAGTAGTCTATTCAGCGACGAGTAGTAATAACCAC16090.13202300433156894No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGCATAATAATAATAAGGCCAAAA16000.1312845288567497No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTACCACCGAGCTGCCCTATC15930.13071015904300143No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTATCCAGCTGCTGCTGCTGC15900.130464000551395No Hit
AGGGTTCCCTGGCCCCAGAAGTCAAATCCCCTGGAGCAGCCAACACCACA15860.13013578922925315No Hit
AGAATTCCCTGGCCCCAGTAGTCATCGCTTTTGGCGCGAGAACAGGTCGC15650.1284126797880083No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAGGGTTGAC15550.12759215148265363No Hit
ATTGTCCCTTGGCCCCAACTATCAAAAGCATCCCCCCGGTTCCAGCCAAC15440.12668957034676348No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATTACC15250.12513056656658958No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACGATTCTATTCCTCTTGCACAGTA15150.12431003826123488No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGCCCCAAC15120.12406387976962847No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGCGGTA15070.12365361561695112No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCATAATAGAAGTAGTCCGTAGT15010.12316129863373833No Hit
GTGGTCCCTAGGCCCCAGACGTCCATACCGTAGGAGTGACCGTAATAAGT15010.12316129863373833No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTTAGATAACCACTCCTT14880.12209461183677722No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACGAGCTGCTATG14880.12209461183677722No Hit
AGGGTTCCCTGGCCCCAGTAATCAAGCTTAGTATTTCCCGCAAGATCTCT14820.12160229485356441No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTAGGTCG14800.12143818919249348No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGACTGACGGTAA14770.12119203070088708No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTCGCCTCCTTGGTACGT14770.12119203070088708No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTCCAGCT14600.11979713258178412No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTACCCCCC14580.11963302692071316No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAGTCCCGGTCCCACTTGCACAGTA14490.11889455144589395No Hit
AGGGTGCCACGGCCCCAGAGATCGAAGTACCAGTACGAGTATAATAACCA14430.11840223446268114No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGCAGTACCTGTTGAATCTTCTCTCGC14410.11823812880161019No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGAGTAGTT14400.11815607597107473No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACTTCGTGGGATCTTT14370.11790991747946833No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGGCCACATATGCCCCTCCAAA14280.11717144200464912No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCATCCCTTACCAGCTGCTGCTAC14230.11676117785197176No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTATCGAAATGACGTAGGGGG14200.11651501936036537No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATGTGATGAGAATACTCCCTG14180.11635091369929441No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACGCAGAATAATAACCACTCCAAAA14110.11577654388554616No Hit
ATTGTCCCTTGGCCCCAGGACGAGTAATAACCACTACTATCATAGCCTCT14010.11495601558019146No Hit
AGGGTTCCCTGGCCCCAGTGAAGAAAGGAGTAGCAGCTACCACTCCATCT13940.11438164576644318No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTACGGGACTTTCCCCGA13920.11421754010537224No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAATGAGTGGAAGG13900.11405343444430131No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTCAAATAACCAGTCAAAATATCG13900.11405343444430131No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTTTGGTT13880.11388932878323038No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAGCTCC13840.11356111746108849No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATGTCC13810.1133149589694821No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTCGACCGACCCTCTCGCACA13780.1130688004778757No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCCGAGAAAGTGACACAATAATA13740.11274058915573382No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGGGTACTCCATA13690.11233032500305645No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAGGGCGGGGAAGGTGATAACTCCC13690.11233032500305645No Hit
AGGGTTCCCTGGCCGCAGTAGTCAAAAGGGGCGTAGAAGCTCCCACTCAC13660.11208416651145006No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGGAGTAACCTGGGTCTGCCCT13640.11192006085037913No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCTATCAGCAGCTGGTACTCCCTG13610.11167390235877271No Hit
AGGGTTCCCTGACTCCAAACGTAATCATTGGCACCATCTTTCGCACAGTA13550.11118158537555992No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTTCCACCCGGCGTAGTCACCGTA13540.11109953254502444No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGATAGCA13530.11101747971448896No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAAACCATAGTAATCCT13510.11085337405341802No Hit
GTGGTCCCTTGGCCCCAGACGTCCAAACCGTAGTAGTGGTCGTCGGGGTA13490.11068926839234709No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCAGCTGCT13420.11011489857859881No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGCGAGCTGAAAAATATC13420.11011489857859881No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTGTCCTCCGTCTCCCACTGC13410.11003284574806335No Hit
AGGGTGCCACGGCCCCAGAGATCGAAGTACCAGTAGCTATAAATACTGGG13370.10970463442592149No Hit
GTGGTCCCTTGGCCCCAGACGTCCATGGCAGCTACCACCACTATTTGACT13330.1093764231037796No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTGGGTTTGCTCTTC13300.1091302646121732No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCCTTTAATAACCAGTCAAA13270.10888410612056679No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTGATTTT13250.10872000045949586No Hit
AGGGTTCCCTGGCCCCAGTAGGCAGAGCAGCCGTTACTAGTACAATAACG13250.10872000045949586No Hit
AGGGTTCCCTGGCCCCACCACTCGAACGTACCAGCCACTGCTTTACTCTC13190.10822768347628303No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATAGAGGTTC13180.10814563064574757No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAAAGGTCCACCTTAATGTA13130.10773536649307024No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGGACTGTAGCCATCTCGAACTCT13100.10748920800146382No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCCCGCCGGAGTTACCACCGTAGT13100.10748920800146382No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAGTTCCCG13080.10732510234039289No Hit
GTTGTCCCTTGGCCCCAGACGTCCATACCGTGAATTCGCGATATAGCAGT13040.106996891018251No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGTCCGCGTAGT13020.10683278535718006No Hit
AGGGTTCCCCGGCCCCAGGGGTCGAACCACCCAAAGCAGCTGGTACGACT13000.10666867969610913No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTGAAGAAAGTAATAACT12980.1065045740350382No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAAGG12940.10617636271289632No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCGGGGTAGTA12930.10609430988236085No Hit
ATTGTCCCTTGGCCCCAGATATCAAAATCATAATTTTTGTAGCCAGGGAG12870.10560199289914804No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTTCAAGGGGAGTAGCAGCTA12860.10551994006861257No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTCCCGTAGTT12830.10527378157700618No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTTCGATAGCAGCTGGTACT12810.10510967591593524No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGACGTTATAATAACT12790.10494557025486428No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAAGAAGAATA12780.10486351742432883No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAACGGGTAGCTCCCACCTCCCGACCG12750.10461735893272242No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTGGGGGGGGCCCCAGCTGTG12740.10453530610218696No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTCTTCACTTACCAGCCA12720.10437120044111603No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGGGTGTTTAGTCACCGTAGTGAG12690.10412504194950961No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGACTCCCGTAGTCAT12670.10396093628843868No Hit
GTGGTCCCTTGGCCCCAGACGTCCAAACCGTAGGAGTGGTAGAAATTTGG12660.1038788834579032No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACAATCGCGGTTGCGAAAGTA12640.10371477779683227No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCTAAGTAGTAATAACCACTACT12640.10371477779683227No Hit
GTGGTCCCTGGGCCCCAGACTTCCATACCGCACGCGTTGCAATTGACATG12590.10330451364415492No Hit
AGGGTTCCCTGGCCCCAGTAGTCTACGGCATTCCCTCCTAAGACTCTCGC12580.10322246081361947No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTAATAGGGTCGGGGAAG12580.10322246081361947No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAATGCTGTC12570.10314040798308399No Hit
AGGGTTCCCTGGCCCCAGTAGTCACCCCCCGCCTGATCACCGTATTCGGT12560.10305835515254853No Hit
AGGGTGCCACGGCCCCAGAGATCGAAGTACCAGCCGTACCAGCTGCTGCT12460.10223782684719382No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAAGGGCCTAACCAGTCAAA12460.10223782684719382No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGCTCCCAAACCCATC12450.10215577401665836No Hit
AGGGTTCCCTGGCCCCAGTAGTCGGGGCCGATAACAATTTCTATCATAGT12420.10190961552505196No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCCAAAAG12380.10158140420291008No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGCTTTTACTACCTCC12380.10158140420291008No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTACCCAGAGGTGGGGCAGCT12360.10141729854183915No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAAAATAAACCATAGCCGC12360.10141729854183915No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCCCCTCGATCGGTCGCTCTCGC12350.10133524571130367No Hit
AGGGTTCCCTGGCCCCAATCGTCGTGGACCGCCACGTAATCACCCTACCA12330.10117114005023274No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAAAAAAGATAACTCCCTCTTCT12320.10108908721969728No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAATAACTCCCCGAACCATAGTAATC12320.10108908721969728No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTAGTGGATCTATCACGGGG12300.10092498155862632No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATCCTCCGGGGGGGTACCAGCTGCT12300.10092498155862632No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTATAACCACTCCAAAAATC12290.10084292872809086No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAGGCCAGC12260.10059677023648446No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGGGAACAACGTAGTTACTGTA12260.10059677023648446No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGCGTCCCCTGTAGCAGCT12240.10043266457541353No Hit
AGGGTTCCCTGGCCCCAGAAGTCTAGGCAGTCACCACCACGATCTCTCGC12240.10043266457541353No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGAACCCCGCCCT12210.10018650608380711No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCTTCGGGCGGGTAACCACTAGA12200.10010445325327165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)