FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001132358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313981
Sequences flagged as poor quality0
Sequence length300
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGATGCT22160.16864779627711513No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAACT19790.1506110057907991No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATTTCCCTCCTCCCGAAC19120.14551199750985747No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAATAACCAGTCAAAATATC18370.1398041524192511No Hit
GTGGTCCCTTTGCCCCAGACGTGCAGAATCCCCGAACCAAAGCCTCTCGC18320.1394236294132107No Hit
ATTGTCCCTTGGCCCCAGACATCAAAAGCATCAGGTAGTAGCTCCCACTC18200.13851037419871368No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACTTTAAGAAGTGTGCGCC17510.13325915671535585No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATGGCCATCGGTAGCAGC17380.13226979689965077No Hit
AGGGTTCCCTGGCCCCAGTACCTCAACCACTCCAAAAATCGTAACCTGAT16150.12290893095105637No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTGCCTCCCAC16020.12191957113535128No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTTGTAGTAGCTAAAATCGTG15920.12115852512327041No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGGCATCGTCGGGTACGGT15830.12047358371239766No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCGGG15710.11956032849790064No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAACCGTAACC15460.11765771346769856No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTTTCGAGGGTTACCACCGAA15460.11765771346769856No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTACGTAGC15400.11720108586045004No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTACGCCCCTATAGCAGC15380.11704887665803386No Hit
ATGATTCCCTGGCCCCAGTAGTCAAAGAAGTAAGAGTAACCGATCAAAAC15280.11628783064595302No Hit
GTGGTCCCTTGGCCCCAGACGTCCACAGCGCCCACCTCCCCGAACCATAC15220.11583120303870452No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAAGGTAGCAG15210.11575509843749644No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGCAGTCAGGTGCTGCTAT15030.11438521561575091No Hit
AGGGTTCCCTGGCGGGAATAATAACCAGTCAAAAGGGCTTTCGCACAGTA14930.11362416960367008No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAAAATAACCACTACTATC14900.11339585580004581No Hit
AGGGTTCCCTGGCCCCAATGGTCAAAGCCCGTTACTCCCACTCTCGATTC14850.11301533279400539No Hit
AGAATTCCCTGGCCCCAGTAGTCAAAGTGGAGGCCCGGTGGTAATATATC14800.11263480978796497No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGTGGTGACGATAATTCCCCCAATTG14790.11255870518675688No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATGGAGTACCATCTGCTGCTCATTCT14690.11179765917467605No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATAATTTTGGGGACAGTGGTGAGCAG14630.11134103156742753No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAATTGGCATA14630.11134103156742753No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCTTCCCCCAAAAAGCCAG14620.11126492696621945No Hit
AGGTTTCCCTGGCCCCAGTGGTCGAACCAGCCTGTCGTTCCACTTATAGA14620.11126492696621945No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCCAGCTG14510.11042777635293052No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCCCGTGAATCCCCAATTATAGCA14500.11035167175172243No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGGTCCAGTCAAAAGATCGTAATAACC14430.10981893954326585No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGACCCCTATAATAACTCCCCGAAC14410.10966673034084969No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGAGCCATAGC14370.10936231193601734No Hit
GTGGTCCCTTGGCCCCAGACGTCCATATCGTAGTAGTAGTCCGTAGTAGG14310.10890568432876883No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATATGTTTTTCGGGTATCTCT14310.10890568432876883No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTAATCTCG14310.10890568432876883No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAATCAAATTT14240.10837295212031224No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCGAAGTA14180.10791632451306374No Hit
AGGGTTCCCTGGCCCCAGTAGTCAACCACTACTATCATAGTACCCGCTCT14160.10776411531064757No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACTGAACTAC14110.10738359230460714No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAAGGTAGCTTACCACCGTA14090.10723138310219098No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGACTTCACAGGTACC14080.1071552785009829No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCCCCCCGACGAGCTGCCG14030.10677475549494247No Hit
GTGGTCCCTAGGCCCCAGACTTCCATGACATAATGGTATATTCCAGTCCC14020.10669865089373438No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGGAAGGACGAGCTGCTATCCCCTCT14010.10662254629252631No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGCCACTGCTCCCCCCTTTTCTCTCGC13970.10631812788769396No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTACAAATTTTCCCCCAA13960.10624202328648588No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTTAGCGTACGTAATCACCGTAGT13960.10624202328648588No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCGTTATCGGGAGAGTAGCAG13920.10593760488165353No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCCCCAAAGGGGTTTGAATAACC13890.10570929107802929No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGATGGTGCTGGAGATCAT13870.10555708187561313No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGCCGTAGTAGATTCCAGC13820.1051765588695727No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTTCCACTCCC13820.1051765588695727No Hit
GTGGTCGTGGGACCTGAGTCCCACCTAGTCCTATTTTTTAGGGCATCCTG13820.1051765588695727No Hit
AGGGTTCCCTGGCCCCAGGGGCCAAACTTCCCGAATGAGAGTAATACGTG13810.10510045426836462No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAAATCGTTCAATATCTCT13730.10449161745869992No Hit
AGAATTCCCTGGCCCCAGTAGTCAAAGCCCCTCCTGGTACATACACCACC13710.10433940825628377No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGGGTATAACCACTCCAAAAACCG13600.10350225764299482No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACGTAGTAGCTCCCACTGTGT13580.10335004844057867No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTGTCGAGCTGCCGAACC13500.102741211630914No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATTGACTCCCCGAACCAT13410.10205627022004124No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAGTAGATT13380.10182795641641698No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCACCCCGGTCATAACCACT13380.10182795641641698No Hit
AGGGTTCCCTGGCCCCAGTAGTCAACGAATCGAGGGGGTCGCCCCTCGCT13350.10159964261279274No Hit
AGGGTTCCCTGGCCCCAGTTGTTTAGTAGTTCCAGTCCCCTCGCACAGTA13290.10114301500554422No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGGACCATAGCTGTATAC13290.10114301500554422No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCGTGGTCAATGTAATCAT13260.10091470120191996No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTACTGAAACACGAAGTTACT13240.1007624919995038No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAAGATACTATCATACCTCC13200.10045807359467145No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)