FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001132376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101
Sequences flagged as poor quality0
Sequence length208
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCGCCTGCACTAAGAGAAACGCAACGAACAACTTTGTCAATGCATTGC3534.65346534653465No Hit
GCCCTGTAATCCCAGCTCCCCGGGAGGCTGAGGTCGGCGGATCGTGGGTG1312.871287128712872No Hit
CGATGTATCCAAGTCTGACGGCCCCAGAAACGGGTGTGCAGGGCGCCCAT1110.891089108910892No Hit
CGGGTCACCTTCTGAATTTTTACCTTCATAAACAGCAAGGACTGCGCTCT65.9405940594059405No Hit
CGGGTCACCTTCTGAATTTTTACCTTCATAAACAGCACGGACTGCGCTCT21.9801980198019802No Hit
CGATGTATCCAGTCTGACGGCCCCAGAAACGGGTGTGCAGGTCGCCCATT10.9900990099009901No Hit
AGGGTTCCCTGGCCCCAGAGGTCGAAGCAGCACCAGATGCGGCGACTACT10.9900990099009901No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTCATAGTAACTCCTGTCCGGACG10.9900990099009901No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCGCACGCCGCGGCAGCTGG10.9900990099009901No Hit
ATTTTTCCCTTTCCCCCTTCTTCCCATTTTCCCTTACTATAACTCCCCCC10.9900990099009901No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCATTCCCCCCCGTACCCTCTTCT10.9900990099009901No Hit
TGTCGCCTGCATAAGAGAAACGCAACGAACAACTTTGTCAATGCATTGCA10.9900990099009901No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTGGTAGCTCAA10.9900990099009901No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACTCGCCCATTGGGCGCAGCT10.9900990099009901No Hit
GCCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATTGCTTGAA10.9900990099009901No Hit
TGTCGCTGCACTAAGAGAAACGCAACGAACAACTTTGTCAATGCATTGCA10.9900990099009901No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCAGTGCGTACTCTCCGTA10.9900990099009901No Hit
GTGGTCCCTTGGCCCCAGACGTCCATATCGTAGTAGGAATAGTAACGGCC10.9900990099009901No Hit
AGGGTTCCCTGGCCCCAGGAGTCGAACCACCACCTGCTGGTACTACTACA10.9900990099009901No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGTCACCGTCGTCATTCCAATTGC10.9900990099009901No Hit
GCCCTGTAATCCCAGCTACTTGGGAGGCTGAGACAGGAGAATCACTTGAA10.9900990099009901No Hit
AGGGTTCCCTGGCCCCAGTAGTCTATGTACCAGTCACTGCTATACCCCCC10.9900990099009901No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTATTTCTCAGAGGT10.9900990099009901No Hit
ACGGTTCCCTGGCCCCAGTAGTCAAAGTCCCAATAGATATGTCTCACACA10.9900990099009901No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGACGCTCCG10.9900990099009901No Hit
CGATGTATCCAAGTCTGACGGAGGCAAGGGAGTGAGTCAAACATATACAT10.9900990099009901No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACTTGCGACCCTCCCTCTC10.9900990099009901No Hit
TGTCGCCTGCACTAAGAGAAGGACTGCGGATCGGAAGAGCGGTTCAGCAG10.9900990099009901Illumina Paired End PCR Primer 2 (95% over 23bp)
GCCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCTCTTGAA10.9900990099009901No Hit
GCCCTGATCCCAGCTCCCCGGGAGGCTGAGGTCGGCGGATCGTGGGTGCT10.9900990099009901No Hit
GCCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCACTCGAA10.9900990099009901No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGCAGAACGAGATCTCACAACTCTA10.9900990099009901No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCTCGTGACACTACTCTCCTA10.9900990099009901No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGTGGAGTATAAGGTGAT10.9900990099009901No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAAGAACGTAGGTACCGGG10.9900990099009901No Hit
GCCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGATTCACTTGAA10.9900990099009901No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATTGCCATAGAAATACGG10.9900990099009901No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTCGTAGTAGTAGTAAAACGA10.9900990099009901No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCCCCGAGGTT10.9900990099009901No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)