FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001132382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176
Sequences flagged as poor quality0
Sequence length208
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAATTTTGGGTTAAGGAGATCGACGCGTTGTCGTTTGTATTAAGAG1910.795454545454545No Hit
GGTTTTTGAGGAATTGGATTTTGAGTTTGTTTGTAAGGTGAGATGTTTTG126.8181818181818175No Hit
GTTTGTAATTTTAGTTTTTTGGGAGGTTGAGGTTGGTGGATTGTGGGTGT116.25No Hit
ATTGTAGAAAGAGTTTGGGAGATTGAGGGGTGTAGAGTGGGGTTGGATTA105.681818181818182No Hit
ATTGTAGAAAGAGTTTGGGAGATTGAGGGGCGTAGAGTGGGGTTGGATTA52.840909090909091No Hit
TTTTTATTTTTATAAATAGTAAGGATTGTGTTTTTTTGTATGGTGTTTTG42.272727272727273No Hit
GTTAAAATTTTGGGTTAAGGAGATCGATGCGTTGTCGTTTGTATTAAGAG42.272727272727273No Hit
GTTTGTAATTTTAGTTTTTTGGGAGGTTGAGGTTGGCGGATTGTGGGTGT42.272727272727273No Hit
GTTAAAATTTTGGGTTAAGGAGATCGACGTGTTGTCGTTTGTATTAAGAG31.7045454545454544No Hit
GTTAAAATTTTGGGTTAAGGAGATTGATGTGTTGTTGTTTGTATTAAGAG31.7045454545454544No Hit
AGGGTTCCCTGGCCCCAGTAGTCGAGTGGTACTACTACAATATCGAGTTC31.7045454545454544No Hit
ATTGTAGAAAGAGTTTGGGAGATCGAGGGGCGTAGAGTGGGGTCGGATTA21.1363636363636365No Hit
ATTGTAGAAAGAGTTTGGGAGGTAAAGGTTGTAGTGAGTCGTGGTCGCGT21.1363636363636365No Hit
GTTTGTAATTTTAGCTTTTTGGGAGGTTGAGGTTGGTGGATTGTGGGTGT21.1363636363636365No Hit
GGTTTTTGAGGAATTGGGTTTTGAGTTTGTTTGTAAGGTGAGATGTTTTG21.1363636363636365No Hit
GTTAAAATTTTGGGTTAAGGAGATTGATGTGTTGTCGTTTGTATTAAGAG21.1363636363636365No Hit
GGTTTTTGAGGAATTGGATTTCGAGTTTGTTCGTAAGGCGAGATGTTTCG21.1363636363636365No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGGAGATAGCAGCTGGTACTAC21.1363636363636365No Hit
ATTGTAGAAAGAGTTTGGGAGATCGAGGGGCGTAGAGTGGGGTTGGATTA21.1363636363636365No Hit
GTTAAAATTTTGGGTTATTTTTTGAATTTTTATTTTGATCGGAAGAGCGG21.1363636363636365No Hit
GGTTTTTGAGGAATTGGATTTCGAGTTTGTTTGTAAGGCGAGACGTTTCG10.5681818181818182No Hit
ATTGTAGAAAGAGTTTGGGAGATCGAGGGGCGGAGAGTGGGGTTGGATTA10.5681818181818182No Hit
GTTAAAATTTTGGGTTAAGGATGTTCGGGGATATTATATTTTTAGTTTTT10.5681818181818182No Hit
GTTAAAACTTTGGGTTGGAAATTTTTTTTTTTAAGAATGTTAAATATTAG10.5681818181818182No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGCCGGTCCACCCTCCCGCACA10.5681818181818182No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAACCAGTTACGCCCCATTGCCCCTCT10.5681818181818182No Hit
GGTTTTTGAGGAATTGGATTTCGAGTTTGTTTGTAAGGCGAGATGTTTCG10.5681818181818182No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTGCATCT10.5681818181818182No Hit
GTTTGTAATTTTAGTTTTTTGGAAGTTCGAGGTAGGCGGGTTATTTGAGG10.5681818181818182No Hit
GTGGTCCCTTGGCCCCAGACGTCCATATTGTAGTAGTAGCACTACCCCCC10.5681818181818182No Hit
GTTAAAATTTTGGGTTAAGGAGATTGACGCGTTGTCGTTTGTATTAAGAG10.5681818181818182No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGGGGCGGATGTGGGCCCTCGTCT10.5681818181818182No Hit
ATTGTAGAAAAGTTTGGGAGATTGAGGGGTGTAGAGTGGGGTTGGATTAG10.5681818181818182No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAATACCAGTACCCGATAAA10.5681818181818182No Hit
GTTTGTAATTTTAGTTTTTTGGGAGGTTGAGGTCGGTGGATTGTGGGTGT10.5681818181818182No Hit
GTTAAAATTTTGGGTTAAGGTGATCGACGCGTTGTCGTTTGTATTAAGAG10.5681818181818182No Hit
GTTAAAATTTTGGGTTAAGGAGATCGACGCGCTGTCGTTTGTATTAAGAG10.5681818181818182No Hit
ATTGTAGAAAGAGTTTAGGAGATTGAGGGGTGTAGAGTGGGGTTGGATTA10.5681818181818182No Hit
AGGGTTCCCTGGCCCCAATAATCAAGGGAAACATAGTTCTGTCCACGTGC10.5681818181818182No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATTGTTG10.5681818181818182No Hit
GGTTTTTGAGGAATTGGATTTTGAGTTTGTTTGTAAGGTGAGATGTTTCG10.5681818181818182No Hit
ACGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCCAACATAGTA10.5681818181818182No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAAACAAATAGTGACCACAGCAGCAGG10.5681818181818182No Hit
ATTGTAGAAAGAGTTTGGGAGTGTGGTCGGTTATTTTGGAGAGGCGGATT10.5681818181818182No Hit
GTTTGTAATTTCAATTTTTTGGGAGGTTGAGGTTGGCGGATTGTGGGTGG10.5681818181818182No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGGAGTAGTACTCTTGAGT10.5681818181818182No Hit
GTGGTCCCTTTGCCCCAGACGTCCATAGGGTAATAACCACTCCAAAAATC10.5681818181818182No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAGGG10.5681818181818182No Hit
TTTTTACCTTTACAAACAGTAAGGATTGTGTTTTTTTGTATGGCGTTTTG10.5681818181818182No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTAATAACGGT10.5681818181818182No Hit
GGTTTTTGAGGAATTGGATTTCGAGTTTGTTCATAAGGCGAGACGTTTCG10.5681818181818182No Hit
GTTAAAATTTTGGGTTAGGAGATCGACGCGTTGTCGTTTGTATTAAGAGA10.5681818181818182No Hit
GTTTGTAATTTTAGTTTTTTGGAAGGTTAAGGTGGGTGGATTACGAGTTT10.5681818181818182No Hit
GTTTGTAATTTTAGTTTTTTATGAGGTTAAGGTTGGAGAATCGTTTGAAT10.5681818181818182No Hit
GTTAAAATCTTGGGTTAAGAGCGTGAATGTGTATTTTTGTTTTTTTTATG10.5681818181818182No Hit
GTTAAAATTTTGGGTTAAGGAGATCGATGTGTTGTTGTTTGTATTAAGAG10.5681818181818182No Hit
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC10.5681818181818182No Hit
GGTTTTTGAGGAAGTGGATTTCGAGTTTGTTCGTAAGGCGAGACGTTTCG10.5681818181818182No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATTCTCGTGAGTCCCCCCAAG10.5681818181818182No Hit
AGGGATCCCTGGCCCCAGTAGTCAAAGGAGAAACAAAAAAAAGTCACACA10.5681818181818182No Hit
GTTAAAATTTTGGGTTAAGGAGATTGGCGTGTTGTCGTTTGTATTAAGAG10.5681818181818182No Hit
GTTTGTAATTTTAGTTTTTTGGAGGTTAAGGTGGGTAGATTATTTGAGGT10.5681818181818182No Hit
AGGGTCCCCTGGCCCCAGAAGTCAAACCAAGAGCAAGCGGAAGAACGAAG10.5681818181818182No Hit
GTTAAAATTTTGGGTTATTTTTTGAATTTTTATTTAGATCGGAAGAGCGG10.5681818181818182No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATAGGAGGACGCACTATC10.5681818181818182No Hit
GTTTGTAATTTTAGTTTTTTGGGAGGTCGAGGTAGGTGGATTATTTGAGG10.5681818181818182No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGAGTAGTAGTAGTTACC10.5681818181818182No Hit
GTTTGTCATTTTAGTTTTTTGGGAGGTTGAGGTTGGTGGATTGTGGGTGT10.5681818181818182No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCCCGCCCC10.5681818181818182No Hit
GTTTGTAATTTTATTTTTTTGGGAGGTTGAGGTTGGTGGATTGTGGGTGT10.5681818181818182No Hit
GTTTGTAATTTTAGTTTTTTGGGCGGTTGAGGTTGGTGGATTGTGGGCGT10.5681818181818182No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTTACAAAAGCAACAGAGCACCTA10.5681818181818182No Hit
GTTTGTAATTTTAGTTTTTTGGGAGGTCGAGGTGGGCGGATTACGAGGTT10.5681818181818182No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCAGACGGGTGACGCTATACC10.5681818181818182No Hit
ATTGTAGAAAGAGTTTGGGAGTGTGGTCGGTTATTTTGGAGAGGCGGTTT10.5681818181818182No Hit
GTTTGTAATTTTAGTTTTTCGCGAGTTTGAGGTAGATGAATTATTTGAAT10.5681818181818182No Hit
GTTTGTAATTTTAGTTTTTTGGGAGGTTGAGGAGGAGGGTGGTTTGAGTT10.5681818181818182No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGCCATCGTCGTAGTAGATCCCACT10.5681818181818182No Hit
ATTGTAGAAAGAGTTTGGGAGGTTGAGGTTGTAGTAAGTTGTGATTGTAG10.5681818181818182No Hit
GGTTTTTGGGAATTGGATTTTGAGTTTGTTTGTAAGGTGAGATGTTTTGT10.5681818181818182No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCCCCCAATAGATAACTCCCC10.5681818181818182No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTTTTGCACGCA10.5681818181818182No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATACCAGAGGCCTCGTAGGCACTATC10.5681818181818182No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGTTGGGGTGT10.5681818181818182No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCACCGGCTTATAATAACC10.5681818181818182No Hit
ATTGTAGAAAGAGTTTGGGAGAGTTAGGGAAGATTTAGGATGAGAGAGAG10.5681818181818182No Hit
AGGGTTCCCTGGCCCCAGTAGCCCCCGAACAAGAACATGTCTACAGTAGC10.5681818181818182No Hit
GTTAAAATTTTGGGTTAAGGAGATTGATGCGTTGTTGTTTGTATTAAGAG10.5681818181818182No Hit
AGGGTTCCCTGGCCCCAGTAGCCCCCGAACAAGACCATGTCTACAGTAGC10.5681818181818182No Hit
TTTTTATTTTTATAAATAGTAAGGATTGTGTTTTTTCGTATGGTGTTTTG10.5681818181818182No Hit
TGGGACCCTGGACTCCACCCTCCCCAAAGCCAGGGGCTATGAGAGGATCA10.5681818181818182No Hit
TTTTTATTTTTATAAATAGTAAGGATTGTTGAATAGTTGTTTGAAGTTTT10.5681818181818182No Hit
ATTGTAGAAAGAGTTTGGGATGGTTTTTAGAAGATCGGAAGAGCGGTTCA10.5681818181818182No Hit
GTTTGTAATTTTAGCCTTTTGGGAGGTTGAGGTTGGCGGATTGTGGGTGT10.5681818181818182No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATGTTACCCC10.5681818181818182No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAAGCAGTTGCCGCTCGCCCCGCAGCC10.5681818181818182No Hit
AGGGTTCCCTGGCCCCAGTAGTGTGGTAGTTCCAGTTTGTCGTCCCAGGG10.5681818181818182No Hit
GGTTTTTGAGGAATTGGATTTTGAGTTTGTTTGCAAGGTGAGATGTTTTG10.5681818181818182No Hit
GTTTGTAATTTTAGTTTTTTGGAAGGTTAAGGTAGGAGGAGTGTTTGAGT10.5681818181818182No Hit
TTTTTATTTTTATAAATAGTAAGGATTGCGTTTTTTTGTATGGTGTTTTG10.5681818181818182No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)