FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785862
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT9177711.678513530365382No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC544216.925007189557454No Hit
CTTACCTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT393815.011185169915329Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT370024.7084602640158195No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT268423.415612410321405No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC244333.1090700402869715No Hit
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC192422.4485214961405437No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG174292.2178194135866094No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT114431.4561080698646836No Hit
CTTACNTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT112911.4367662515810664Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT91441.1635630683249731No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC86281.0979026852042726No Hit
CTTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT83221.058964551028043Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG79631.0132822302134472No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT79321.0093375172740253No Hit
CTTCCCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT69440.8836156984305131Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT61720.7853796213584573Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG60560.7706187600367495No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG50020.6364985200964037No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT48440.6163932089858015No Hit
CTTACNTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG39720.5054322514639975No Hit
CTTACCTGAGGAGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAA35330.4495700262896031Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTACCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT33200.42246603093163937Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC32440.4127951217898308No Hit
CTTACNTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT31630.4024879686255348No Hit
CTTACCTGAGGAGACGGTGACCAGATCGGAAGAGCACACGTCTGAACTCC29490.3752567244630737Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAAC29120.37054851869666683Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTACCTGAGGAGACGGTGACCTGCTGTCTCTTTTCGGAGCGGGACGCCG28250.35947787270538595No Hit
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT27950.3556604085704615No Hit
CTTCCNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT24050.3060333748164436Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA23360.2972532073061173No Hit
CTTACCTGAGGAGACGGTGACCGAGGTCGAGGGGCTGGCGGAAGGGACAG21190.2696402167301638No Hit
TTTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT19970.254115862581471No Hit
CTTACCTGAGGAGACTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT19670.2502983984465466Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCCCAACATGCGGCCTTTACCCTGGGGAGG18970.2413909821317229No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG18950.24113648452272793No Hit
CTTCCNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT18550.23604653234282863Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC18400.23413780027536643No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC17880.22752086244149736No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT17080.21734095808169882No Hit
CTTACNTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT16940.21555947481873408No Hit
CTTACNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT16430.2090697857893625Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCACGAAGGCGGCAAAGGCGACGGAATGGA15340.195199666099137No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG15140.19265469000918736No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT14220.18094779999541905No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC13730.17471260857504245No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA12830.1632602161702691No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC11980.1524440677879831No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG11540.14684512039009393No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG9920.12623081406150188No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG9880.12572181884351197No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC9770.12432208199403966No Hit
CTTACCTGGGGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAACT9510.12101361307710515Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG9470.1205046178591152No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACG9390.11948662742313534No Hit
TTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC9330.11872313459615047No Hit
CTTACNTGAGGAGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAA9310.11846863698715551Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTACNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT9230.11745064655117567Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCAGATCGGAAGAGCACACGTCTGAACTCC9020.11477842165672854Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT8980.1142694264387386No Hit
CTTACNTGAGGAGACGGTGACCTGCTGTCTCTTTTCGGAGCGGGACGCCG8740.11121545513079906No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA8530.10854323023635193No Hit
CTTACCTGAGGAGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC8350.10625275175539725Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT8240.10485301490592495No Hit
ATTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT8150.10370777566544763No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA8020.10205354120698036No Hit
CTTACNTGAGGGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAAC7990.10167179479348791Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)