FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586926
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT10017817.068250511989586No Hit
CTTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT6203110.568794021733574Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT386946.592653929115425No Hit
CTTACCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT256934.377553558710979Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC211283.5997723733486No Hit
CTTCCNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT179563.0593294555020565Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCCCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT158622.7025553476928947Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT122522.0874863270667854Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT108941.85611133260411No Hit
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG108351.8460589580287803No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC97791.6661384910533865No Hit
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC71531.2187226328361667No Hit
CTTACNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT69791.1890766468004486Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG67321.1469929769681357No Hit
CTTACCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT58340.9939924283470147Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG51170.8718305203722446No Hit
CTTCCNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT44550.7590394700524427Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT38350.653404347396435No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT38120.6494856251043573No Hit
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC35220.6000756483781601No Hit
CTTACNTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT34730.5917270661037336Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT33410.5692370077318094No Hit
CTTACCTGAGGAGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC28250.48132132500519653Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACNTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG23650.40294687916364247No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT21980.3744935477385565No Hit
CTTACCTGAGGAGACGGTGACCAGATCGGAAGAGCACACGTCTGAACTCC21340.363589277012775Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG19350.3296838102247983No Hit
CTTACCTGAGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT19280.32849115561416603Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGGACCAGATCGGAAGAGCACACGTCTGAACTC17380.29611910189700236Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT15460.263406289719658Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT15380.2620432558789353Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC12630.21518896760409317No Hit
CTTCCCTGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC12050.20530697225885375Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT11720.1996844576658727No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA11470.19542497691361432No Hit
ATTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT11110.18929132463036227No Hit
CTTACNTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT10140.17276453931159974No Hit
CTTCCCTGGGGGCGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC10040.1710607470106964Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACCTGAGGGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAAC9010.1535116863113919Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTACCTGAGGAGACTGCCTGATCCAAGATCGGAAGAGCACACGTCTGAA8930.15214865247066922Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTCCCTGGGGTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC8400.14311855327588147Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACNTGAGGAGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC8030.13681452176253905Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGCGGACCAGATCGGAAGAGCACACGTCTGAACTC7330.12488797565621561Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACNTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT7010.11943584029332488No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG6950.11841356491278288No Hit
CTTACNTGAGGAGACGGTGACCAGATCGGAAGAGCACACGTCTGAACTCC6820.1161986349216085Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC6810.11602825569151819No Hit
ATTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT6790.1156874972313375Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG6700.1141540841605245No Hit
CTTACCTGAGGAGACGGTGACCAAGATCGGAAGAGCACACGTCTGAACTC6660.11347256724016316Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACCTGAGGAGACGGTGACCACAGGCGGAGAACACTGACAGCATCCTT6510.11091687878880814No Hit
CTTACCTGAGGAGACGGACCAAGATCGGAAGAGCACACGTCTGAACTCCA6050.10307943420465272Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTACCTGAGGAGACGGTGACCTGCTGTCTCTTTTCGGAGCGGGACGCCG5940.10120526267365904No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)