FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001132398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433723
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT7822818.036396501914815Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT6523215.040014018163667No Hit
CTTACNTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT263716.080147928516587No Hit
CTTCCNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT226865.230527318127008Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT152323.5119188975452076Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCCCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT122912.8338363425504296Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC122882.8331446568431926No Hit
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG70011.614163878788997No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT62891.4500038042713899No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC53531.2341978636134123No Hit
CTTACCTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT45501.0490566559762797Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC43010.9916467422755999No Hit
CTTACNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT41960.9674377425223011Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG37790.8712934292163431No Hit
CTTCCNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT35540.8194170011735601Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG34830.8030471061022819No Hit
CTTCCCTGGGGGCGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC29260.6746241264585923Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT26670.6149085937337886No Hit
CTTACCTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT26610.6135252223193144Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT22960.5293701279387997No Hit
CTTTCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT22950.5291395660363873Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCCCTGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC21250.4899440426262845Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACCTGAGGAGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC19610.452131890630656Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACNTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC18370.42354221473152215No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT16720.3854995008334813No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT13920.3209421681580179No Hit
TTTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT13300.30664733020845103No Hit
CTTACNTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG13120.3024972159650284No Hit
CTTACNTGAGGAGCCTGGCGGACCAGATCGGAAGAGCACACGTCTGAACT12970.2990387874288428Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGATCGGAAGAGCACACGTCTGAACTCC12070.2782882162117296Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG12040.2775965305044925No Hit
CTTCCCTGGGGTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC11930.27506034957795644Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT9980.23010077860754446Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT9360.21580594065797754Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCCCTGGGGGTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC9140.21073357880490545Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTACCTGAGGAGACGGTGGACCAGATCGGAAGAGCACACGTCTGAACTC8640.19920548368428698Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTCCNTGGGGGCGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC8510.1962081789529262Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT8170.18836907427090563Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACNTGGGGGCTGGCGGATCCAAGATCGGAAGAGCACACGTCTGAACT7110.16392951261519448Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC6880.15862658885971No Hit
CTTACNTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT6800.15678209364041104No Hit
CTTACNTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT5960.13741489383777203No Hit
CTTCCNTGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTC5840.1346481510088236Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTCNTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT5810.1339564653015865Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTACCTGAGGAGACGGTGACCACAGGTGGAGAACACTGACAGCATCCTT5630.12980635105816385No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC5500.12680904632680304No Hit
CTTACNTGAGGAGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC5490.12657848442439068Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCCCTGGGGCGACGGATCCAAGATCGGAAGAGCACACGTCTGAACTCC5360.12358117969302987Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCCCTGGGGGCTGACGGATCCAAGATCGGAAGAGCACACGGCTGAACT5330.12288949398579277Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA4990.11505038930377223No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG4950.11412814169412275No Hit
CTTACNTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT4870.11228364647482378No Hit
CTTACCTGAGGAGACGGACCAAGATCGGAAGAGCACACGTCTGAACTCCA4730.10905577984105061Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTACCTGAGGAGACGGTGACCTGCTGTCTCTTTTCGGAGCGGGACGCCG4490.10352229418315376No Hit
CTTCCCTGAGGGCTGACGGATCCAAGATCGGAAGAGCACACGTCTGAACT4420.10190836086626717Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCCCTGGGGGCTGACGGATCCAAGATCGGAAGCGCACACGTCTGAACT4400.10144723706144243Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)