FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424065
Sequences flagged as poor quality0
Sequence length300
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCTCCTGGGCCTAGGCCGCCTCCTTCATCC79751.880607925671772No Hit
CTTACCTGAGGAGACGGTGACCCTGTGGGCTAAGGTCTCCCTTTGGCCAG64421.5191067407119192No Hit
CTTACCTGAGGAGACGGTGACCTGGGCTTCCCCTGAGGGCAGAGCTCTGA50291.185903104476908No Hit
CTTACCTGAGGAGACGGTGACCCACAAGGGCTGGCCCCTGAATGCAGCAG44341.0455944253829013No Hit
CTTACCTGAGGAGACGGTGACCAGCACCAGCCCTGGGGGAGAAGAGGCTC42070.9920648957117423No Hit
CTTACCTGAGGAGACGGTGACCAGGAGCAGACAGGGGAGGGGCTGCTGGG40200.9479678822821974No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG36900.8701496232888826No Hit
CTTACCTGAGGAGACGGTGACCCTGCCTAGGATGTCCTATCTTAAGGTGA34450.8123754613089975No Hit
CTTACCTGAGGAGACGGTGACCTGGGGACAGGGACAGGTGGTCCTGGGAT33170.7821914093358329No Hit
CTTACCTGAGGAGACGGTGACCGTGGGCAGGGGCTTCCAAGCCAACAGGG29720.700835956751913No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC28890.6812634855505642No Hit
CTTACCTGAGGAGACGGTGACCCACAGTGTGACACTTCGCAGGGACCAGG28690.6765472274297573No Hit
CTTACCTGAGGAGACGGTGACCATGCAGACACCACACGCTCTCCTGCAGT28170.6642849563156592No Hit
CTTACCTGAGGAGACGGTGACCTCTGGCGGGTGTAGAAGAGCTCATGCTT27860.6569747562284084No Hit
CTTACCTGAGGAGACGGTGACCATGGGAGGGGGCTGCAGAGAGTCTCAGA27280.6432976076780682No Hit
CTTACCTGAGGAGACGGTGACCCAGGCCAGCCCACCACGTTTGGGACTCT26070.6147642460471862No Hit
CTTACCTGAGGAGACGGTGACCCCTCTCTCTGGTTCCCAGCACCTGCCTG25760.6074540459599354No Hit
CTTACCTGAGGAGACGGTGACCCCCAGGCCTCTCTCCTCTCAAGGGGCTG25180.5937768974095952No Hit
CTTACCTGAGGAGACGGTGACCTCTGTGGCTGCTGCACGGCACAGCCGAG21980.518316767476684No Hit
CTTACCTGAGGAGACGGTGACCTGGGGGTGGGTTTTCAAGAAGGGCAGGC21410.5048754318323843No Hit
CTTACCTGAGGAGACGGTGACCTTGCAGTGAGCTGGTAAGCAAGGCCAGA19870.4685602443021707No Hit
CTTACCTGAGGAGACGGTGACCAGGACCAGGATGGGATTTCATAGCAGGC19520.46030679259075846No Hit
CTTACCTGAGGAGACGGTGACCTCTGAGCAAGGGGCTCGAGAGTCCCAGG19370.4567695990001533No Hit
CTTACCTGAGGAGACGGTGACCCAGTCAGAAAAGCTGAAAGAAAATTGAA18850.4445073278860552No Hit
CTTACCTGAGGAGACGGTGACCCAGACTCAGGGCTCAGGGTGACTGTCCC18510.43648968908068336No Hit
CTTACCTGAGGAGACGGTGACCTGGCCTTGGAAGGACGGGCTGTATCTGG17880.42163347600014145No Hit
CTTACCTGAGGAGACGGTGACCCTGGAAATGGGGCCGACCTGATGTGGAC17680.4169172178793345No Hit
CTTACCTGAGGAGACGGTGACCAGGTCAAAGGCTGTCCCTCGGGCCTCAG17670.4166814049732942No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCAGGCTG17620.4155023404430924No Hit
CTTACCTGAGGAGACGGTGACCCGGCAGCAGCACATGGCACCCGCGCAGA17480.41220095975852755No Hit
CTTACCTGAGGAGACGGTGACCAAGGTGGTGGCGGTGGACGGCGACTCGG16260.3834317852216052No Hit
CTTACCTGAGGAGACGGTGACCTGAGCACCCCCTGGTGTTCTGAGCTTCC16120.38013040453704033No Hit
CTTACCTGAGGAGACGGTGACCCCAGCACCCACAGCCTGCCTATGGGGCT14540.3428719653826654No Hit
CTTACCTGAGGAGACGGTGACCGACAGCGCTGGGTGAAATCCCGCACCGT14380.3390989588860199No Hit
CTTACCTGAGGAGACGGTGACCGCGACAACATCTCCCATCCACGGGCTCT14350.3383915201678988No Hit
CTTACCTGAGGAGACGGTGACCGCCGCGGTGGAGGAGCTTTTGGTGGCCG14310.3374482685437374No Hit
CTTACCTGAGGAGACGGTGACCTGCTGCTGTGACTTCCTCTCCAGAGCTG14080.33202457170480937No Hit
CTTACCTGAGGAGACGGTGACCCAACTGCTGATGGGCATCGAGCAGGCCC13170.31056559725513777No Hit
CTTACCTGAGGAGACGGTGACCCCAACATGCGGCCTTTACCCTGGGGAGG13130.3096223456309764No Hit
CTTACCTGAGGAGACGGTGACCTCAGCGACGGCGCTTCGTCGGGAGTCTC12730.3001898293893625No Hit
CTTACCTGAGGAGACGGTGACCCCCCCAAGCTATCCTGAGTAGGAGAGCA12700.29948239067124144No Hit
CTTACCTGAGGAGACGGTGACCCTGGACTCCCACTGTGTGTGCCAGCGGA12600.29712426161083794No Hit
CTTACCTGAGGAGACGGTGACCTCTCTCCAGGCTCTAAGGGAGAGCGGGT12110.28556942921486095No Hit
CTTACCTGAGGAGACGGTGACCCGCTTGCAGTATCTCCCAGTTTCACCAC11410.2690625257920366No Hit
CTTACCTGAGGAGACGGTGACCGGCACTCCGGAGGCCTGTCCTGGCTGTC11150.26293139023498757No Hit
CTTACCTGAGGAGACGGTGACCATCCCTGAGTGTGTGCACATTCCACCCT10820.25514956433565605No Hit
CTTACCTGAGGAGACGGTGACCTCCGCCCCATCCAAGAAGGGCTGGGAGG10710.25255562236921225No Hit
CTTACCTGAGGAGACGGTGACCCCAGGCTGTCCTGCAGGACCAGGGACAA10620.2504333062148491No Hit
CTTACCTGAGGAGACGGTGACCTTCCCCTTCCCCTTCGTCCCCACTCACT9720.22921014467121784No Hit
CTTACCTGAGGAGACGGTGACCCAGTTGGCCAGTAGCCCACCCAGATCTT9210.21718368646316014No Hit
CTTACCTGAGGAGACGGTGACCGGGCAGCACAAGGCAAGCATCTCAGAGC8840.20845860893966725No Hit
CTTACCTGAGGAGACGGTGACCTTGCGCACCTTCTCCAGCAGCTTGCTCA8760.2065721056913445No Hit
CTTACCTGAGGAGACGGTGACCCACCCCTCACATCCTCTCTCCCTGAGTC8690.20492141534906205No Hit
CTTACCTGAGGAGACGGTGACCCCGCTGCATCCCATACCCTCCACCCATC8490.2002051572282551No Hit
CTTACCTGAGGAGACGGTGACCCTGTGGTCTCTTCCAACCCCGAGGCTGT8430.19879027979201302No Hit
CTTACCTGAGGAGACGGTGACCACACGGCGCAGCTGCTTTGCACCCGGGA8300.1957247120134885No Hit
CTTACCTGAGGAGACGGTGACCCACGAGGGAGCAGGGTGACACCCATTGG8230.19407402167120608No Hit
CTTACCTGAGGAGACGGTGACCTGTCCTTAGAAGACGACCAGAGTGGCTC8220.19383820876516572No Hit
CTTACCTGAGGAGACGGTGACCGTGACGCGAGGCCCCGTATGTCACGCGT8060.19006520226852017No Hit
CTTACCTGAGGAGACGGTGACCCTGTCTGTAAAAAAAAGTTCTTAATTTT8030.1893577635503991No Hit
CTTACCTGAGGAGACGGTGACCAAGAAAAGGGCCAGAGGCCGCAGGCTGA7880.1858205699597939No Hit
CTTACCTGAGGAGACGGTGACCATCAGGGCGCCTGTGGCACCGTTGCCAG7760.18299081508730974No Hit
CTTACCTGAGGAGACGGTGACCTAACTGGTTTGTCCCCACTGTCTCCTCT7640.18016106021482556No Hit
CTTACCTGAGGAGACGGTGACCCCTGTCCTTCTCACGGGGGGTCTCCAGC7600.17921780859066416No Hit
CTTACCTGAGGAGACGGTGACCCGGGCGCTACAGGACACCTGCCCCAAGG7310.1723792343154941No Hit
CTTACCTGAGGAGACGGTGACCACAGAGATGATGGCGCCAACAGCTGCCA7150.16860622781884851No Hit
CTTACCTGAGGAGACGGTGACCCCAGAGAGTGTGGAGACGGAGAGTCCTT7040.16601228585240468No Hit
CTTACCTGAGGAGACGGTGACCTCAGCCAAGCTCTCAGCCCCTCTGAGCC7000.16506903422824332No Hit
CTTACCTGAGGAGACGGTGACCGGAGGGTGTGACCATGGAGGCGCCGCAG6940.16365415679200124No Hit
CTTACCTGAGGAGACGGTGACCTCTGTGAAAGGCTCCCCAGACTCATCGT6500.15327838892622594No Hit
CTTACCTGAGGAGACGGTGACCACGATTGTATGACAATAAAATGGACCTT6410.1511560727718628No Hit
CTTACCTGAGGAGACGGTGACCTGAGCACCCCCTGGTGTCCTGAGCTTCC6230.14691144046313653No Hit
CTTACCTGAGGAGACGGTGACCCGGCAAGGCCCTTACCGATCTGGTCGCT6230.14691144046313653No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGGGGCTCTGGTGCCGGGCTGAG6210.14643981465105585No Hit
CTTACCTGAGGAGACGGTGACCTCTGAGTCCCCAGGAGAGCCCGAGGCTC6190.14596818883897517No Hit
CTTACCTGAGGAGACGGTGACCTGAAACTGCGTGGATCTGCTGTTTCCAT6130.14455331140273306No Hit
CTTACCTGAGGAGACGGTGACCGTGAAGATGTCTGGGGCGGGGATTCCAA6120.14431749849669273No Hit
CTTACCTGAGGAGACGGTGACCCCTCCCTCGTGGCTCAGTAAGTGTGGCC6020.14195936943628926No Hit
CTTACCTGAGGAGACGGTGACCTGTGGCGCTGCGCTGTGTGATGATTGCT5750.13559242097319985No Hit
CTTACCTGAGGAGACGGTGACCTGGTTCTGAAGTGTGTACTCAGACGTGA5730.13512079516111916No Hit
CTTACCTGAGGAGACGGTGACCCGGCCCAGCTAGGAGCAGGTGGGGGGCC5680.13394173063091744No Hit
CTTACCTGAGGAGACGGTGACCAGGCTAGGGCCCTTGGTACGGGCTGAGG5570.1313477886644736No Hit
CTTACCTGAGGAGACGGTGACCTCTCTGTGGAATCCAAGTGTCTCAGTCC5540.13064034994635257No Hit
CTTACCTGAGGAGACGGTGACCACCCTGGGCATGGGCCCGCCTGGCCTCG5490.12946128541615085No Hit
CTTACCTGAGGAGACGGTGACCCCACACACCCCCGCAGGACCCTCGGTCT5470.12898965960407013No Hit
CTTACCTGAGGAGACGGTGACCTGGCCTCCACCCTCTGGAAGCTAGCGCT5460.1287538466980298No Hit
CTTACCTGAGGAGACGGTGACCTCCTCCTTCGACTGCTCCCTGGTGACGC5450.12851803379198945No Hit
CTTACCTGAGGAGACGGTGACCCTGCGTTGTGTGGCCCGTGGCAGCCCCC5440.1282822208859491No Hit
CTTACCTGAGGAGACGGTGACCCACGGGGGCGCCGGCCTTGCCAGCTCTC5200.12262271114098075No Hit
CTTACCTGAGGAGACGGTGACCACCACATCTGAACACAGCGGGCCCCGGC5190.1223868982349404No Hit
CTTACCTGAGGAGACGGTGACCGACCCCGAGAAGCTCAACAACTACGAGC5190.1223868982349404No Hit
CTTACCTGAGGAGACGGTGACCTGCTGGAGCGTCGTGCCAGCATGGGCTG4960.11696320139601239No Hit
CTTACCTGAGGAGACGGTGACCACCATCTTCATGGCCTGTTCCCATGAGG4780.11271856908728614No Hit
CTTACCTGAGGAGACGGTGACCCAGATGCACAGGGTCCTCTACTTGAGAC4730.11153950455708442No Hit
CTTACCTGAGGAGACGGTGACCCGGAGAGGGACGGGAAAGCTCCTGGTGG4650.10965300130876164No Hit
CTTACCTGAGGAGACGGTGACCAGACTCAAGTTACTGATCCTCCGCTGTT4650.10965300130876164No Hit
CTTACCTGAGGAGACGGTGACCCAGGGACACCACACTCGGTGTCAGCCTC4640.10941718840272129No Hit
CTTACCTGAGGAGACGGTGACCACGGGAAGAGTCCCCGGGAGGAGGAGCC4580.1080023109664792No Hit
CTTACCTGAGGAGACGGTGACCCTTCTGCGGGGCTCTGGACAAGCAGCAG4510.10635162062419676No Hit
CTTACCTGAGGAGACGGTGACCCAGGAGGGAGGGAGAGCTTGGCTCCGAG4450.10493674318795468No Hit
CTTACCTGAGGAGACGGTGACCTGGGAGTGAGTACAAGGTGAGGCCACCA4430.10446511737587398No Hit
CTTACCTGAGGAGACGGTGACCCCCGGACGCTCCAGCATGCAGCCCCAGG4390.10352186575171259No Hit
CTTACCTGAGGAGACGGTGACCAAGAGGCCTTGTGAGCGCGTAGCATCTT4320.10187117540943016No Hit
CTTACCTGAGGAGACGGTGACCCCCAACATATTCCGGCCTTCTGGGAAAT4310.10163536250338981No Hit
CTTACCTGAGGAGACGGTGACCAATGCAGCCTTGGGGCGGCCGACTCCTT4300.10139954959734947No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)