FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480024
Sequences flagged as poor quality0
Sequence length300
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC27052256.355932203389834No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGGGGTCGAACC96512.0105244737763113No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC91421.904488108927887No Hit
CTTACCTGAGGAGACGGTGACCATGCAGACACCACACGCTCTCCTGCAGT71351.4863840141326266No Hit
CTTACCTGAGGAGACGGTGACCCAGGAAGGGGCTCTGGTGCCGGGCTGAG70821.4753428995216906No Hit
CTTACCTGAGGAGACGGTGACCATTTTTAGCCCTTGGCTAGGTGTGGGGC26220.5462226888655567No Hit
CTTACCTGAGGAGACGGTGACCGACGTGCTGTAGACGCGCAGGCCGCTCA24790.516432511707748No Hit
CTTACCTGAGGAGACGGTGACCCCATCCACTTCTCAGGGAACAGGGCATT23560.4908087928936887No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCGAACC19330.40268819892338714No Hit
CTTACCTGAGGAGACGGTGACCTAACTGGTTTGTCCCCACTGTCTCCTCT18790.3914387613952635No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG14320.29831841741246273No Hit
CTTACCTGAGGAGACGGTGACCCAGGCCAGCCCACCACGTTTGGGACTCT14300.2979017715780878No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCGAACC13560.2824858757062147No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT13040.271653084012466No Hit
CTTACCTGAGGAGACGGTGACCCACAAGGGCTGGCCCCTGAATGCAGCAG12060.2512374381280936No Hit
CTTACCTGAGGAGACGGTGACCGACAGCGCTGGGTGAAAAACCGCACCGT9960.20748962551872407No Hit
CTTACCTGAGGAGACGGTGACCAGACTCAAGTTACTGATCCTCCGCTGTT9440.19665683382497542No Hit
CTTACCTGAGGAGACGGTGACCTAGAGGCACAGGTCCGCCTCCAGCTCCT9380.19540689632185057No Hit
CTTACCTGAGGAGACGGTGACCTCTGTGGCTGCTGCACGGCACAGCCGAG9330.19436528173591322No Hit
CTTACCTGAGGAGACGGTGACCTGGCCTTGGAAGGACGGGCTGTATCTGG8900.18540739629685182No Hit
CTTACCTGAGGAGACGGTGACCTCCTGGGCCTAGGCCGCCTCCTTCATCC8870.1847824275452894No Hit
CTTACCTGAGGAGACGGTGACCCGCTTGCAGTATCTCCCAGTTTCACCAC8670.18061596920153994No Hit
CTTACCTGAGGAGACGGTGACCTCTGGCGGGTGTAGAAGAGCTCATGCTT8490.17686615669216538No Hit
CTTACCTGAGGAGACGGTGACCAGGCTAGGGCCCTTGGTACGGGCTGAGG8440.17582454210622803No Hit
CTTACCTGAGGAGACGGTGACCATGCCACTGGACACCTCTGGTGCTGACT8360.17415795876872822No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACT8000.16665833374997918No Hit
CTTACCTGAGGAGACGGTGCCCAGGGTTCCCTGGCCCCAGGGGTCGAACC7960.16582504208122928No Hit
CTTACCTGAGGAGACGGTGACCACACGGCGCAGCTGCTTTGCACCCGGGA7910.1647834274952919No Hit
CTTACCTGAGGAGACGGTGACCACCATCATCGCTCCGCCGGGCCACACAG7880.16415845874372947No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGGGGTCGAACC7470.15561721913904303No Hit
CTTACCTGAGGAGACGGTGACCACCATCTTCATGGCCTGTTCCCATGAGG7190.14978417745779377No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCAAACC7080.14749262536873156No Hit
CTTACCTGAGGAGACGGTGACCTCTCTCCAGGCTCTAAGGGAGAGCGGGT7000.14582604203123178No Hit
CTTACCTGAGGAGACGGTGACCAAGGTGGTGGCGGTGGACGGCGACTCGG6910.1439511357765445No Hit
CTTACCTGAGGAGACGGTGACCGTGACGCGAGGCCCCGTATGTCACGCGT6380.13291002116560838No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC6140.12791027115310902No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC6020.1254103961468593No Hit
CTTACCTGAGGAGACGGTGACCGGGTGAAATCTATCAGACTGCAGGGAGA5730.11936903154842259No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCCGGCCCCAGGGGTCGAACC5630.11728580237654784No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGCACC5510.11478592737029815No Hit
CTTACCTGAGGAGACGGTGACCCCGGGTGGTGAGACCTTAGGCTGTGAGC5500.11457760445311067No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTTGCCCCAGGGGTCGAACC5340.11124443777811108No Hit
CTTACCTGAGGAGACGGTGACCGGGCAGCACAAGGCAAGCAGCTCAGAGC5230.10895288568904889No Hit
CTTACCTGAGGAGACGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACCA5080.10582804193123678No Hit
CTTACCTGAGGAGACGGTGACCACACACTGTCATGCGGGGAGCGTGTAGC4980.10374481275936204No Hit
CTTACCTGAGGAGACGGTGACCCCCAGGCCTCTCTCCTCTCAAGGGGCTG4940.10291152109061213No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAGC4930.10270319817342467No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGCCGAACC4840.10082829191873739No Hit
CTTACCTGAGGAGACGGTGACCGGGGTTCCCTGGCCCCAGGGGTCGAACC4830.10061996900154993No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)