FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001132431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1217857
Sequences flagged as poor quality0
Sequence length300
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT21634617.76448302222675No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC17752714.576998777360561No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC1043328.566851444791958No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT927357.614604998780646No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT792986.511273491058474No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG589914.843836345318047No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT250712.058616077257018No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG86700.7119062418658348No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGTAGTCAAAGT77470.63611737667066No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT72550.595718544952322No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC69050.5669795386486263No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT59600.4893842216286477No Hit
CTTACCTGAGGAGACGGTGANCGTGGTCCCTTGGCCCCAGACGTCCATAC55770.4579355375877463No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG53630.44036368801920095No Hit
NTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC48340.3969267327773293No Hit
CTTACCTGAGGAGACGGTGACCAGGCCTGCTGTCCCCCAGGTAAGGACAG45920.3770557627044883No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCC44530.36564227162959195No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA38460.31580062355432537No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGGGGTCGAACC36990.30373024090677314No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC33390.2741701201372575No Hit
CTTACCTGAGGAGACGGTGANCAGGGTGCCCTGGCCCCAGTGCTGGAAGT32000.2627566290623612No Hit
NTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT29310.24066864993180642No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC28100.23073316489538592No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA27590.22654548111970454No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC27330.22441058350857285No Hit
CTTACCTGAGGAGACGGTGANCGTGGTCCCTTTGCCCCAGACGTCCATGT25870.2124223123076026No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACT25620.21036952614305293No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGG25220.2070850682797734No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGC23190.19041644462362986No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG22780.18704987531376838No Hit
NTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT22520.18491497770263668No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC20700.16997069442471488No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT20130.1652903419695416No Hit
CTTACCTGAGGAGACGGTGANCATTGTCCCTTGGCCCCAGATATCAAAAG20040.1645513389503037No Hit
CTTACCTGAGGAGACGGTGACCCCACCTGCCTTTAATTAAAAGCTCAGTC19410.15937831781563844No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACTCG19160.15732553165108876No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT19110.1569149744181788No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACAGT18820.15453374246730117No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGCCAGGTA18460.1515777303903496No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACAC18440.15141350749718563No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT17870.14673315504201231No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG17790.14607626346935643No Hit
NTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG17580.14435192309113468No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGCCTATAGCAGCTGGTAC17350.14246335981974895No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATCCACTGGT16630.13655133566584582No Hit
CTTACCTGAGGAGACGGTGACCAGGGTCCCCTGGCCCCAGTAGTCAAAGG15670.12866863679397497No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCGC15450.12686218496917126No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA15240.1251378445909495No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGTTCGAGCT14770.12127860660159609No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGGACTGTA14720.12086804936868616No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCCAGAGCCCAATT14710.12078593792210417No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCCGGGGAGG14160.11626980836009482No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTTCTCAC14110.11585925112718488No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAG14050.11536658244769296No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCAAAC13950.11454546798187308No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC13900.11413491074896313No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCATTA13870.11388857640921718No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCACT13830.11356013062288922No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCGCTTA13580.11150734445833953No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCAGCAGATAACGGGGG13510.11093256433226562No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGCTGGAAGT13450.11043989565277369No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGACC13340.10953666974037181No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCGT13320.10937244684720783No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACTC13030.10699121489633019No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC12990.10666276911000223No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACCCAAAGC12750.10469209439203453No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGCC12710.10436364860570659No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACACA12690.1041994257125426No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAAGCAGCTG12670.10403520281937863No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGACCCCCGTTC12590.10337831124672273No Hit
CTTACCTGAGGAGACGGTGACCATGGTCCCTTGGCCCCAGACGTCCATAC12210.1002580762766072No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)