FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001132432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411152
Sequences flagged as poor quality0
Sequence length300
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT28418020.13815662664263No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC19974214.154534734741544No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT1334309.455395308230438No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC913616.474213975532048No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG527713.739568806195222No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT286892.033019830606483No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT160821.1396362688073292No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC155061.0988185539190676No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGTAGTCAAAGT99090.7021922514371237No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT96150.6813582094629069No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC84130.596179575269No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT70990.5030641631801536No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG70350.49852886152590226No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA66010.4677738471830108No Hit
CTTACCTGAGGAGACGGTGANCGTGGTCCCTTGGCCCCAGACGTCCATAC63680.45126251459800215No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG60930.43177489030239125No Hit
NTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC54810.38840606823361334No Hit
CTTACCTGAGGAGACGGTGANCAGGGTGCCCTGGCCCCAGTGCTGGAAGT47880.33929725500867375No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG47180.3343367688243364No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG46330.3283133213147839No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC45400.32172296109845006No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT43270.306628910280395No Hit
NTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT41840.29649534564667734No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC38100.2699921766046464No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT35970.2548981257865914No Hit
CTTACCTGAGGAGACGGTGACCAGGCCTGCTGTCCCCCAGGTAAGGACAG33390.2366151909928909No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACCCC31570.22371792691361383No Hit
CTTACCTGAGGAGACGGTGANCAGGGTTCCCTGGCCCCAGGGGTCGAACC31320.22194632470492193No Hit
NTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC30740.21783620758075672No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC29580.20961597333242626No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT28860.2045137589713936No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA26990.19126217445037813No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACTA26090.18488440649908727No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA22070.1563970429833214No Hit
CTTACCTGAGGAGACGGTGACCCCACCTGCCTTTAATTAAAAGCTCAGTC21730.15398766397950045No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCTGCGG20830.14760989602820956No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAAAGCCCTATAC20690.14661779879134212No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC20140.14272027393221992No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGGGG19970.14151558443030943No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGTTT19760.1400274385750082No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCTTTGTCTA19430.13768892365953492No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCACGAACCTTAGCA19370.13726373912944884No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCGGACTGGTTTACC19070.13513781647901857No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGCTGGAAGT18980.13450003968388946No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACGT18720.1326575733868499No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTACCACC18710.13258670929850222No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCCCCC18690.1324449811218069No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCTAGC18320.12982300985294284No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACGCA18220.12911436896946607No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCGTCGC18210.1290435048811184No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG17630.12493338775695319No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCCCGG17620.12486252366860551No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCTACCGACTACCA17520.12415388278512875No Hit
CTTACCTGAGGAGACGGTGANCATTGTCCCTTGGCCCCAGATATCAAAAG17430.12351610598999967No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGC17390.12323264963660895No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCCGGG17340.12287832919487057No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTAACC17320.12273660101817523No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAC17220.12202796013469847No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGTTTCTCTGCGTAGTCAC17170.12167363969296006No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGAGT16960.12018549383765886No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC16730.11855561980566233No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAATACG16500.11692574577366578No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCTAGTCACACAGTA16500.11692574577366578No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAA16390.11614624080184134No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGCG16390.11614624080184134No Hit
NTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG16310.11557932809505991No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGGAATAGC16000.11338254135628197No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTATTGAAGCA15970.11316994909123894No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGACTG15930.11288649273784822No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCTCTTCGCCGGACT15890.11260303638445752No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGTTTC15180.10757168611177251No Hit
CTTACCTGAGGAGACGGTGACCATGGTCCCTTGGCCCCAGACGTCCATAC15110.10707563749333877No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGACCATAC15070.10679218113994807No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATTT15070.10679218113994807No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGACCCGTG14720.1043119380477794No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCACAGCTGGTCCTA14680.10402848169438869No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCGGGCGGGTTTGT14570.10324897672256425No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCGGCCAGTAGTCCTAG14550.10310724854586892No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTATAGGAAAA14530.10296552036917356No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCATTTCCGCCACT14240.10091046180709094No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTCCTTGCTCTAGCTCCCAC14170.10041441318865722No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)