FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001132435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294532
Sequences flagged as poor quality0
Sequence length300
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC57248944.223626762412984No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC579784.478684188571623No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT518804.007625921954807No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC454593.51161655331811No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG371812.872157660065568No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT250841.9376886782250264No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG113950.8802408901440829No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG109700.8474104927495033No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG98950.7643688993396841No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT97180.750696004424765No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC72220.5578850117262455No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC71900.5554130759224183No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA54160.41837513479774924No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG42300.32675901406840463No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCAGGCTG37990.29346512871060737No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT29220.22571863808696888No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC26990.20849233545404827No Hit
CTTACCTGAGGAGACGGTGACCTGGGGACAGGGACAGGTGGTCCTGGGAT26560.20517067171765552No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG25140.1942014565881724No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT24590.18995281692534446No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT22530.174039730188207No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA21960.16963659453763985No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC21420.1654652028686815No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG20510.15843563542654795No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA19540.15094258002119684No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATAC19270.14885688418671766No Hit
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG18900.1459987084135425No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACG18370.14190456473845373No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT18280.14120933279362735No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT18110.13989611689784417No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG17400.1344115093331026No Hit
CTTACCTGAGGAGACGGTGCCCGTGGTCCCTTGGCCCCAGACGTCCATAC16770.12954488571931788No Hit
CTTACCTGAGGAGACGGTGACCCTGTGGGCTAAGGTCTCCCTTTGGCCAG16380.1265322139584035No Hit
CTTACCTGAGGAGACGGTGACCCACAGTGTGACACTTCGCAGGGACCAGG15790.12197458232009716No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGCCCATAC15610.12058411843044436No Hit
CTTACCTGAGGAGACGGTGACCGTGGCCCCTTGGCCCCAGACGTCCATAC15600.12050687043657476No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG15590.12042962244270516No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGCC14780.11417253493926764No Hit
CTTACCTGAGGAGACGGTGACCAGCACCAGCCCTGGGGGAGAAGAGGCTC14460.11170059913544045No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC14450.11162335114157085No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACT14320.11061912722126607No Hit
CTTACCTGAGGAGACGGTGACCCGGCAGCAGCACATGGCACCCGCGCAGA13750.1062159915706989No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGC13290.10266258385269736No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)